Substrate-Dependent and Organ-Specific Chloroplast Protein Import in Planta. Kim, C. and Apel, K. The Plant Cell Online, 16(1):88--98, January, 2004.
Substrate-Dependent and Organ-Specific Chloroplast Protein Import in Planta [link]Paper  doi  abstract   bibtex   
The NADPH-dependent protochlorophyllide (Pchlide) oxidoreductase (POR) is unique because it is a photoenzyme that requires light for its catalytic activity and uses Pchlide itself as a photoreceptor. In Arabidopsis, there are three structurally related PORs, denoted PORA, PORB, and PORC. The import of one of them, PORA, into plastids of cotyledons is substrate dependent. This substrate dependence is demonstrated in intact seedlings of wild-type Arabidopsis and two mutants, xantha2, which is devoid of Pchlide, and flu, which upon redarkening rapidly accumulates Pchlide. In true leaves, PORA uptake does not require the presence of Pchlide. The organ specificity of the substrate-dependent import of PORA reveals a means of controlling plastid protein translocation that is closely associated with a key step in plant development, the light-dependent transformation of cotyledons from a storage organ to a photosynthetically active leaf.
@article{ kim_substrate-dependent_2004,
  title = {Substrate-Dependent and Organ-Specific Chloroplast Protein Import in Planta},
  volume = {16},
  issn = {1040-4651, 1532-298X},
  url = {http://www.phgoods.info/content/16/1/88.short},
  doi = {10.1105/tpc.015008},
  abstract = {The {NADPH}-dependent protochlorophyllide (Pchlide) oxidoreductase ({POR}) is unique because it is a photoenzyme that requires light for its catalytic activity and uses Pchlide itself as a photoreceptor. In Arabidopsis, there are three structurally related {PORs}, denoted {PORA}, {PORB}, and {PORC}. The import of one of them, {PORA}, into plastids of cotyledons is substrate dependent. This substrate dependence is demonstrated in intact seedlings of wild-type Arabidopsis and two mutants, xantha2, which is devoid of Pchlide, and flu, which upon redarkening rapidly accumulates Pchlide. In true leaves, {PORA} uptake does not require the presence of Pchlide. The organ specificity of the substrate-dependent import of {PORA} reveals a means of controlling plastid protein translocation that is closely associated with a key step in plant development, the light-dependent transformation of cotyledons from a storage organ to a photosynthetically active leaf.},
  number = {1},
  urldate = {2012-02-16},
  journal = {The Plant Cell Online},
  author = {Kim, Chanhong and Apel, Klaus},
  month = {January},
  year = {2004},
  pages = {88--98}
}
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