@article{RN103,
   author = {Kim, J. and Zhao, Y. and Yang, S. and Feng, Z. and Wang, A. and Davalos, R. V. and Jia, X.},
   title = {Laser Machined Fiber-based Microprobe: Application in Microscale Electroporation},
   journal = {Adv Fiber Mater},
   volume = {4},
   number = {4},
   pages = {859-872},
   note = {2524-793x
Kim, Jongwoon
Zhao, Yajun
Yang, Shuo
Feng, Ziang
Wang, Anbo
Davalos, Rafael V
Jia, Xiaoting
R01 CA213423/CA/NCI NIH HHS/United States
R01 NS123069/NS/NINDS NIH HHS/United States
R21 EY033080/EY/NEI NIH HHS/United States
Journal Article
Singapore
2022/08/01
Adv Fiber Mater. 2022 Aug;4(4):859-872. doi: 10.1007/s42765-022-00148-5. Epub 2022 Mar 23.},
   abstract = {Microscale electroporation devices are mostly restricted to in vitro experiments (i.e., microchannel and microcapillary). Novel fiber-based microprobes can enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to electroporate. We developed a flexible, fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining techniques. The fiber consists of four copper electrodes (80 μm), one microfluidic channel (30 μm), and has an overall diameter of 400 μm. The dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser setup. The feasibility of the fiber probe was validated through numerical simulations and in vitro experiments. Successful reversible and irreversible microscale electroporation was observed in a 3D collagen scaffold (seeded with U251 human glioma cells) using fluorescent staining. The ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical simulations. The computational and experimental results of the working fiber-based microprobe suggest the feasibility of in vivo microscale electroporation in space-sensitive areas, such as the deep brain.},
   keywords = {fiber probe
irreversible electroporation
micromachine
microscale electroporation
polymer
reversible electroporation},
   ISSN = {2524-7921 (Print)
2524-7921},
   DOI = {10.1007/s42765-022-00148-5},
   year = {2022},
   type = {Journal Article}
}

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V.","Jia, X."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Kim"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Zhao"],"firstnames":["Y."],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Feng"],"firstnames":["Z."],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Jia"],"firstnames":["X."],"suffixes":[]}],"title":"Laser Machined Fiber-based Microprobe: Application in Microscale Electroporation","journal":"Adv Fiber Mater","volume":"4","number":"4","pages":"859-872","note":"2524-793x Kim, Jongwoon Zhao, Yajun Yang, Shuo Feng, Ziang Wang, Anbo Davalos, Rafael V Jia, Xiaoting R01 CA213423/CA/NCI NIH HHS/United States R01 NS123069/NS/NINDS NIH HHS/United States R21 EY033080/EY/NEI NIH HHS/United States Journal Article Singapore 2022/08/01 Adv Fiber Mater. 2022 Aug;4(4):859-872. doi: 10.1007/s42765-022-00148-5. Epub 2022 Mar 23.","abstract":"Microscale electroporation devices are mostly restricted to in vitro experiments (i.e., microchannel and microcapillary). Novel fiber-based microprobes can enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to electroporate. We developed a flexible, fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining techniques. The fiber consists of four copper electrodes (80 μm), one microfluidic channel (30 μm), and has an overall diameter of 400 μm. The dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser setup. The feasibility of the fiber probe was validated through numerical simulations and in vitro experiments. Successful reversible and irreversible microscale electroporation was observed in a 3D collagen scaffold (seeded with U251 human glioma cells) using fluorescent staining. The ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical simulations. The computational and experimental results of the working fiber-based microprobe suggest the feasibility of in vivo microscale electroporation in space-sensitive areas, such as the deep brain.","keywords":"fiber probe irreversible electroporation micromachine microscale electroporation polymer reversible electroporation","issn":"2524-7921 (Print) 2524-7921","doi":"10.1007/s42765-022-00148-5","year":"2022","bibtex":"@article{RN103,\n author = {Kim, J. and Zhao, Y. and Yang, S. and Feng, Z. and Wang, A. and Davalos, R. V. and Jia, X.},\n title = {Laser Machined Fiber-based Microprobe: Application in Microscale Electroporation},\n journal = {Adv Fiber Mater},\n volume = {4},\n number = {4},\n pages = {859-872},\n note = {2524-793x\nKim, Jongwoon\nZhao, Yajun\nYang, Shuo\nFeng, Ziang\nWang, Anbo\nDavalos, Rafael V\nJia, Xiaoting\nR01 CA213423/CA/NCI NIH HHS/United States\nR01 NS123069/NS/NINDS NIH HHS/United States\nR21 EY033080/EY/NEI NIH HHS/United States\nJournal Article\nSingapore\n2022/08/01\nAdv Fiber Mater. 2022 Aug;4(4):859-872. doi: 10.1007/s42765-022-00148-5. Epub 2022 Mar 23.},\n abstract = {Microscale electroporation devices are mostly restricted to in vitro experiments (i.e., microchannel and microcapillary). Novel fiber-based microprobes can enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to electroporate. We developed a flexible, fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining techniques. The fiber consists of four copper electrodes (80 μm), one microfluidic channel (30 μm), and has an overall diameter of 400 μm. The dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser setup. The feasibility of the fiber probe was validated through numerical simulations and in vitro experiments. Successful reversible and irreversible microscale electroporation was observed in a 3D collagen scaffold (seeded with U251 human glioma cells) using fluorescent staining. The ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical simulations. 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