Transport of organic substrates via a volume-activated channel. Kirk, K., Ellory, J. C., & Young, J. D. The Journal of Biological Chemistry, 267(33):23475–23478, November, 1992. abstract bibtex We have investigated the volume-activated transport of organic solutes in flounder erythrocytes. Osmotic swelling of cells suspended in a Na(+)-free medium led to increased membrane transport of taurine, glucose, and uridine. For each compound there was a significant lag period (1-2 min at 10 degrees C) between cell swelling and activation of the flux. The volume-activated fluxes of each of the substrates increased in parallel with increasing cell volume, and those of taurine and uridine increased linearly with concentration (up to 19 mM). The volume-activated fluxes of each of the three compounds showed similar sensitivities to a number of anion-selective channel blockers (5-nitro-2-(3-phenylpropylamino)benzoic acid \textgreater 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid approximately MK-196 \textgreater niflumic acid \textgreater furosemide); the IC50 for the inhibition of the volume-activated fluxes by NPPB was around 12 microM. The results are consistent with the hypothesis that the volume-activated transport of organic osmolytes is via a pathway with the characteristics of a volume-activated "chloride channel." This raises the question of whether the transport of organic substrates might represent a physiological role for such channels in other cell types.
@article{kirk_transport_1992,
title = {Transport of organic substrates via a volume-activated channel},
volume = {267},
issn = {0021-9258},
abstract = {We have investigated the volume-activated transport of organic solutes in flounder erythrocytes. Osmotic swelling of cells suspended in a Na(+)-free medium led to increased membrane transport of taurine, glucose, and uridine. For each compound there was a significant lag period (1-2 min at 10 degrees C) between cell swelling and activation of the flux. The volume-activated fluxes of each of the substrates increased in parallel with increasing cell volume, and those of taurine and uridine increased linearly with concentration (up to 19 mM). The volume-activated fluxes of each of the three compounds showed similar sensitivities to a number of anion-selective channel blockers (5-nitro-2-(3-phenylpropylamino)benzoic acid {\textgreater} 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid approximately MK-196 {\textgreater} niflumic acid {\textgreater} furosemide); the IC50 for the inhibition of the volume-activated fluxes by NPPB was around 12 microM. The results are consistent with the hypothesis that the volume-activated transport of organic osmolytes is via a pathway with the characteristics of a volume-activated "chloride channel." This raises the question of whether the transport of organic substrates might represent a physiological role for such channels in other cell types.},
language = {eng},
number = {33},
journal = {The Journal of Biological Chemistry},
author = {Kirk, K. and Ellory, J. C. and Young, J. D.},
month = nov,
year = {1992},
pmid = {1385424},
keywords = {Pleuronectiformes},
pages = {23475--23478},
}
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