Transcriptional profiling of cells sorted by RNA abundance. Klemm, S., Semrau, S., Wiebrands, K., Mooijman, D., Faddah, D., A., Jaenisch, R., & van Oudenaarden, A. Nature methods, 11 VN - r(5):549-551, 2014.
Paper
Website abstract bibtex We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requiring antibodies or transgenic fluorescent proteins.
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