Magnify is a universal molecular anchoring strategy for expansion microscopy. Klimas, A., Gallagher, B. R., Wijesekara, P., Fekir, S., DiBernardo, E. F., Cheng, Z., Stolz, D. B., Cambi, F., Watkins, S. C., Brody, S. L., Horani, A., Barth, A. L., Moore, C. I., Ren, X., & Zhao, Y. Nature biotechnology, 41(6):858–869, June, 2023. Place: United Statesdoi abstract bibtex Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion microscopy protocols require prior treatment with reactive anchoring chemicals to link specific labels and biomolecule classes to the gel. We describe a strategy called Magnify, which uses a mechanically sturdy gel that retains nucleic acids, proteins and lipids without the need for a separate anchoring step. Magnify expands biological specimens up to 11 times and facilitates imaging of cells and tissues with effectively around 25-nm resolution using a diffraction-limited objective lens of about 280 nm on conventional optical microscopes or with around 15 nm effective resolution if combined with super-resolution optical fluctuation imaging. We demonstrate Magnify on a broad range of biological specimens, providing insight into nanoscopic subcellular structures, including synaptic proteins from mouse brain, podocyte foot processes in formalin-fixed paraffin-embedded human kidney and defects in cilia and basal bodies in drug-treated human lung organoids.
@article{klimas_magnify_2023,
title = {Magnify is a universal molecular anchoring strategy for expansion microscopy.},
volume = {41},
copyright = {© 2023. The Author(s).},
issn = {1546-1696 1087-0156},
doi = {10.1038/s41587-022-01546-1},
abstract = {Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion microscopy protocols require prior treatment with reactive anchoring chemicals to link specific labels and biomolecule classes to the gel. We describe a strategy called Magnify, which uses a mechanically sturdy gel that retains nucleic acids, proteins and lipids without the need for a separate anchoring step. Magnify expands biological specimens up to 11 times and facilitates imaging of cells and tissues with effectively around 25-nm resolution using a diffraction-limited objective lens of about 280 nm on conventional optical microscopes or with around 15 nm effective resolution if combined with super-resolution optical fluctuation imaging. We demonstrate Magnify on a broad range of biological specimens, providing insight into nanoscopic subcellular structures, including synaptic proteins from mouse brain, podocyte foot processes in formalin-fixed paraffin-embedded human kidney and defects in cilia and basal bodies in drug-treated human lung organoids.},
language = {eng},
number = {6},
journal = {Nature biotechnology},
author = {Klimas, Aleksandra and Gallagher, Brendan R. and Wijesekara, Piyumi and Fekir, Sinda and DiBernardo, Emma F. and Cheng, Zhangyu and Stolz, Donna B. and Cambi, Franca and Watkins, Simon C. and Brody, Steven L. and Horani, Amjad and Barth, Alison L. and Moore, Christopher I. and Ren, Xi and Zhao, Yongxin},
month = jun,
year = {2023},
pmid = {36593399},
pmcid = {PMC10264239},
note = {Place: United States},
keywords = {*Kidney, *Microscopy/methods, Animals, Humans, Mice},
pages = {858--869},
}
Downloads: 0
{"_id":"ob3a9fZiuojzb3bzr","bibbaseid":"klimas-gallagher-wijesekara-fekir-dibernardo-cheng-stolz-cambi-etal-magnifyisauniversalmolecularanchoringstrategyforexpansionmicroscopy-2023","author_short":["Klimas, A.","Gallagher, B. R.","Wijesekara, P.","Fekir, S.","DiBernardo, E. F.","Cheng, Z.","Stolz, D. B.","Cambi, F.","Watkins, S. C.","Brody, S. L.","Horani, A.","Barth, A. L.","Moore, C. I.","Ren, X.","Zhao, Y."],"bibdata":{"bibtype":"article","type":"article","title":"Magnify is a universal molecular anchoring strategy for expansion microscopy.","volume":"41","copyright":"© 2023. The Author(s).","issn":"1546-1696 1087-0156","doi":"10.1038/s41587-022-01546-1","abstract":"Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion microscopy protocols require prior treatment with reactive anchoring chemicals to link specific labels and biomolecule classes to the gel. We describe a strategy called Magnify, which uses a mechanically sturdy gel that retains nucleic acids, proteins and lipids without the need for a separate anchoring step. Magnify expands biological specimens up to 11 times and facilitates imaging of cells and tissues with effectively around 25-nm resolution using a diffraction-limited objective lens of about 280 nm on conventional optical microscopes or with around 15 nm effective resolution if combined with super-resolution optical fluctuation imaging. We demonstrate Magnify on a broad range of biological specimens, providing insight into nanoscopic subcellular structures, including synaptic proteins from mouse brain, podocyte foot processes in formalin-fixed paraffin-embedded human kidney and defects in cilia and basal bodies in drug-treated human lung organoids.","language":"eng","number":"6","journal":"Nature biotechnology","author":[{"propositions":[],"lastnames":["Klimas"],"firstnames":["Aleksandra"],"suffixes":[]},{"propositions":[],"lastnames":["Gallagher"],"firstnames":["Brendan","R."],"suffixes":[]},{"propositions":[],"lastnames":["Wijesekara"],"firstnames":["Piyumi"],"suffixes":[]},{"propositions":[],"lastnames":["Fekir"],"firstnames":["Sinda"],"suffixes":[]},{"propositions":[],"lastnames":["DiBernardo"],"firstnames":["Emma","F."],"suffixes":[]},{"propositions":[],"lastnames":["Cheng"],"firstnames":["Zhangyu"],"suffixes":[]},{"propositions":[],"lastnames":["Stolz"],"firstnames":["Donna","B."],"suffixes":[]},{"propositions":[],"lastnames":["Cambi"],"firstnames":["Franca"],"suffixes":[]},{"propositions":[],"lastnames":["Watkins"],"firstnames":["Simon","C."],"suffixes":[]},{"propositions":[],"lastnames":["Brody"],"firstnames":["Steven","L."],"suffixes":[]},{"propositions":[],"lastnames":["Horani"],"firstnames":["Amjad"],"suffixes":[]},{"propositions":[],"lastnames":["Barth"],"firstnames":["Alison","L."],"suffixes":[]},{"propositions":[],"lastnames":["Moore"],"firstnames":["Christopher","I."],"suffixes":[]},{"propositions":[],"lastnames":["Ren"],"firstnames":["Xi"],"suffixes":[]},{"propositions":[],"lastnames":["Zhao"],"firstnames":["Yongxin"],"suffixes":[]}],"month":"June","year":"2023","pmid":"36593399","pmcid":"PMC10264239","note":"Place: United States","keywords":"*Kidney, *Microscopy/methods, Animals, Humans, Mice","pages":"858–869","bibtex":"@article{klimas_magnify_2023,\n\ttitle = {Magnify is a universal molecular anchoring strategy for expansion microscopy.},\n\tvolume = {41},\n\tcopyright = {© 2023. The Author(s).},\n\tissn = {1546-1696 1087-0156},\n\tdoi = {10.1038/s41587-022-01546-1},\n\tabstract = {Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion microscopy protocols require prior treatment with reactive anchoring chemicals to link specific labels and biomolecule classes to the gel. We describe a strategy called Magnify, which uses a mechanically sturdy gel that retains nucleic acids, proteins and lipids without the need for a separate anchoring step. Magnify expands biological specimens up to 11 times and facilitates imaging of cells and tissues with effectively around 25-nm resolution using a diffraction-limited objective lens of about 280 nm on conventional optical microscopes or with around 15 nm effective resolution if combined with super-resolution optical fluctuation imaging. We demonstrate Magnify on a broad range of biological specimens, providing insight into nanoscopic subcellular structures, including synaptic proteins from mouse brain, podocyte foot processes in formalin-fixed paraffin-embedded human kidney and defects in cilia and basal bodies in drug-treated human lung organoids.},\n\tlanguage = {eng},\n\tnumber = {6},\n\tjournal = {Nature biotechnology},\n\tauthor = {Klimas, Aleksandra and Gallagher, Brendan R. and Wijesekara, Piyumi and Fekir, Sinda and DiBernardo, Emma F. and Cheng, Zhangyu and Stolz, Donna B. and Cambi, Franca and Watkins, Simon C. and Brody, Steven L. and Horani, Amjad and Barth, Alison L. and Moore, Christopher I. and Ren, Xi and Zhao, Yongxin},\n\tmonth = jun,\n\tyear = {2023},\n\tpmid = {36593399},\n\tpmcid = {PMC10264239},\n\tnote = {Place: United States},\n\tkeywords = {*Kidney, *Microscopy/methods, Animals, Humans, Mice},\n\tpages = {858--869},\n}\n\n\n","author_short":["Klimas, A.","Gallagher, B. R.","Wijesekara, P.","Fekir, S.","DiBernardo, E. F.","Cheng, Z.","Stolz, D. B.","Cambi, F.","Watkins, S. C.","Brody, S. L.","Horani, A.","Barth, A. L.","Moore, C. I.","Ren, X.","Zhao, Y."],"key":"klimas_magnify_2023","id":"klimas_magnify_2023","bibbaseid":"klimas-gallagher-wijesekara-fekir-dibernardo-cheng-stolz-cambi-etal-magnifyisauniversalmolecularanchoringstrategyforexpansionmicroscopy-2023","role":"author","urls":{},"keyword":["*Kidney","*Microscopy/methods","Animals","Humans","Mice"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://neuronex1.github.io/pubs.bib","dataSources":["TvqYudBJxx7Hha9Hu"],"keywords":["*kidney","*microscopy/methods","animals","humans","mice"],"search_terms":["magnify","universal","molecular","anchoring","strategy","expansion","microscopy","klimas","gallagher","wijesekara","fekir","dibernardo","cheng","stolz","cambi","watkins","brody","horani","barth","moore","ren","zhao"],"title":"Magnify is a universal molecular anchoring strategy for expansion microscopy.","year":2023}