@ARTICLE{Klyueva2017574,
author={Klyueva, N.Z. and Rudenko, E.D. and Aldekeeva, A.S. and Plekhanov, A.Y. and Korneva, N.A. and Petrova, E.I.},
title={The impact of high salt consumption on the renal metabolism of NAP-22 and MARCKS, major protein kinase C substrates, in spontaneously hypertensive rats},
journal={Arterial Hypertension (Russian Federation)},
year={2017},
volume={23},
number={6},
pages={574-580},
doi={10.18705/1607-419X-2017-23-6-574-580},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041110186&doi=10.18705%2f1607-419X-2017-23-6-574-580&partnerID=40&md5=857bca81359840a7fc7bb096c679d5ec},
affiliation={Pavlov Institute of Physiology, 6 Makarova emb., Saint-Petersburg, 199034, Russian Federation; Institute for Analytical Instrumentation, St Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},
abstract={Objective. To investigate changes in NAP-22 messenger RNA (mRNA) expression level and the level of NAP-22 protein, as well as the level of MARCKS protein in kidney cells cytosole in spontaneously hypertensive rats after long-term high salt consumption which is a dietary factor of arterial hypertension (HTN). Design and methods. We evaluated SHR and WKY male rats before and after 10-day consumption of 1 % NaCl instead of drinking water. NAP-22 mRNA level was estimated using real time polymerase chain reaction (PCR), and the content of NAP-22 and MARCKS proteins was evaluated by dot immunoblotting or electerophoresis with subsequent immunoblotting. Results. In spontaneously hypurtensive rats, the renal level of NAP-22 mRNA expression was significantly increased as compared with WKY group. High NaCl consumption definitely diminished NAP-22 mRNA expression in the both rat lines, however, its level was still higher in hypertensive rats than in normotensive rats. The NAP-22 protein level decreased, too. Similar changes were observed in the level of MARCKS protein in renal tissue. Conclusions. Unlike the long-term exogenous calcium deficiency, the salt load leads to the decrease of both RNA expression and the content in major protein kinase C (PK C) substrate proteins (NAP-22 and MARCKS). This indicates a potential change in the functioning of renal PK C system with an increased consumption of NaCl.},
author_keywords={Kidney;  MARCKS;  NAP-22;  Salt charge;  Shr and wky rat lines;  Spontaneous hypertension},
correspondence_address1={Klyueva, N.Z.; Pavlov Institute of Physiology, 6 Makarova emb., Russian Federation; email: natklueva@mail.ru},
publisher={All-Russian Public Organization Antihypertensive League},
issn={1607419X},
language={Russian},
abbrev_source_title={Arter. Hypertens.},
document_type={Article},
source={Scopus},
}

{"_id":"ZbiA8RnuXH576C44k","bibbaseid":"klyueva-rudenko-aldekeeva-plekhanov-korneva-petrova-theimpactofhighsaltconsumptionontherenalmetabolismofnap22andmarcksmajorproteinkinasecsubstratesinspontaneouslyhypertensiverats-2017","author_short":["Klyueva, N.","Rudenko, E.","Aldekeeva, A.","Plekhanov, A.","Korneva, N.","Petrova, E."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Klyueva"],"firstnames":["N.Z."],"suffixes":[]},{"propositions":[],"lastnames":["Rudenko"],"firstnames":["E.D."],"suffixes":[]},{"propositions":[],"lastnames":["Aldekeeva"],"firstnames":["A.S."],"suffixes":[]},{"propositions":[],"lastnames":["Plekhanov"],"firstnames":["A.Y."],"suffixes":[]},{"propositions":[],"lastnames":["Korneva"],"firstnames":["N.A."],"suffixes":[]},{"propositions":[],"lastnames":["Petrova"],"firstnames":["E.I."],"suffixes":[]}],"title":"The impact of high salt consumption on the renal metabolism of NAP-22 and MARCKS, major protein kinase C substrates, in spontaneously hypertensive rats","journal":"Arterial Hypertension (Russian Federation)","year":"2017","volume":"23","number":"6","pages":"574-580","doi":"10.18705/1607-419X-2017-23-6-574-580","note":"cited By 1","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041110186&doi=10.18705%2f1607-419X-2017-23-6-574-580&partnerID=40&md5=857bca81359840a7fc7bb096c679d5ec","affiliation":"Pavlov Institute of Physiology, 6 Makarova emb., Saint-Petersburg, 199034, Russian Federation; Institute for Analytical Instrumentation, St Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation","abstract":"Objective. To investigate changes in NAP-22 messenger RNA (mRNA) expression level and the level of NAP-22 protein, as well as the level of MARCKS protein in kidney cells cytosole in spontaneously hypertensive rats after long-term high salt consumption which is a dietary factor of arterial hypertension (HTN). Design and methods. We evaluated SHR and WKY male rats before and after 10-day consumption of 1 % NaCl instead of drinking water. NAP-22 mRNA level was estimated using real time polymerase chain reaction (PCR), and the content of NAP-22 and MARCKS proteins was evaluated by dot immunoblotting or electerophoresis with subsequent immunoblotting. Results. In spontaneously hypurtensive rats, the renal level of NAP-22 mRNA expression was significantly increased as compared with WKY group. High NaCl consumption definitely diminished NAP-22 mRNA expression in the both rat lines, however, its level was still higher in hypertensive rats than in normotensive rats. The NAP-22 protein level decreased, too. Similar changes were observed in the level of MARCKS protein in renal tissue. Conclusions. Unlike the long-term exogenous calcium deficiency, the salt load leads to the decrease of both RNA expression and the content in major protein kinase C (PK C) substrate proteins (NAP-22 and MARCKS). This indicates a potential change in the functioning of renal PK C system with an increased consumption of NaCl.","author_keywords":"Kidney; MARCKS; NAP-22; Salt charge; Shr and wky rat lines; Spontaneous hypertension","correspondence_address1":"Klyueva, N.Z.; Pavlov Institute of Physiology, 6 Makarova emb., Russian Federation; email: natklueva@mail.ru","publisher":"All-Russian Public Organization Antihypertensive League","issn":"1607419X","language":"Russian","abbrev_source_title":"Arter. Hypertens.","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Klyueva2017574,\r\nauthor={Klyueva, N.Z. and Rudenko, E.D. and Aldekeeva, A.S. and Plekhanov, A.Y. and Korneva, N.A. and Petrova, E.I.},\r\ntitle={The impact of high salt consumption on the renal metabolism of NAP-22 and MARCKS, major protein kinase C substrates, in spontaneously hypertensive rats},\r\njournal={Arterial Hypertension (Russian Federation)},\r\nyear={2017},\r\nvolume={23},\r\nnumber={6},\r\npages={574-580},\r\ndoi={10.18705/1607-419X-2017-23-6-574-580},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041110186&doi=10.18705%2f1607-419X-2017-23-6-574-580&partnerID=40&md5=857bca81359840a7fc7bb096c679d5ec},\r\naffiliation={Pavlov Institute of Physiology, 6 Makarova emb., Saint-Petersburg, 199034, Russian Federation; Institute for Analytical Instrumentation, St Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},\r\nabstract={Objective. To investigate changes in NAP-22 messenger RNA (mRNA) expression level and the level of NAP-22 protein, as well as the level of MARCKS protein in kidney cells cytosole in spontaneously hypertensive rats after long-term high salt consumption which is a dietary factor of arterial hypertension (HTN). Design and methods. We evaluated SHR and WKY male rats before and after 10-day consumption of 1 % NaCl instead of drinking water. NAP-22 mRNA level was estimated using real time polymerase chain reaction (PCR), and the content of NAP-22 and MARCKS proteins was evaluated by dot immunoblotting or electerophoresis with subsequent immunoblotting. Results. In spontaneously hypurtensive rats, the renal level of NAP-22 mRNA expression was significantly increased as compared with WKY group. High NaCl consumption definitely diminished NAP-22 mRNA expression in the both rat lines, however, its level was still higher in hypertensive rats than in normotensive rats. The NAP-22 protein level decreased, too. Similar changes were observed in the level of MARCKS protein in renal tissue. Conclusions. Unlike the long-term exogenous calcium deficiency, the salt load leads to the decrease of both RNA expression and the content in major protein kinase C (PK C) substrate proteins (NAP-22 and MARCKS). This indicates a potential change in the functioning of renal PK C system with an increased consumption of NaCl.},\r\nauthor_keywords={Kidney; MARCKS; NAP-22; Salt charge; Shr and wky rat lines; Spontaneous hypertension},\r\ncorrespondence_address1={Klyueva, N.Z.; Pavlov Institute of Physiology, 6 Makarova emb., Russian Federation; email: natklueva@mail.ru},\r\npublisher={All-Russian Public Organization Antihypertensive League},\r\nissn={1607419X},\r\nlanguage={Russian},\r\nabbrev_source_title={Arter. Hypertens.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n","author_short":["Klyueva, N.","Rudenko, E.","Aldekeeva, A.","Plekhanov, A.","Korneva, N.","Petrova, E."],"key":"Klyueva2017574","id":"Klyueva2017574","bibbaseid":"klyueva-rudenko-aldekeeva-plekhanov-korneva-petrova-theimpactofhighsaltconsumptionontherenalmetabolismofnap22andmarcksmajorproteinkinasecsubstratesinspontaneouslyhypertensiverats-2017","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041110186&doi=10.18705%2f1607-419X-2017-23-6-574-580&partnerID=40&md5=857bca81359840a7fc7bb096c679d5ec"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lbp_2019_10.txt","dataSources":["6vPsRbdrk6L6BBWH9"],"keywords":[],"search_terms":["impact","high","salt","consumption","renal","metabolism","nap","marcks","major","protein","kinase","substrates","spontaneously","hypertensive","rats","klyueva","rudenko","aldekeeva","plekhanov","korneva","petrova"],"title":"The impact of high salt consumption on the renal metabolism of NAP-22 and MARCKS, major protein kinase C substrates, in spontaneously hypertensive rats","year":2017}