@article{knowles_genome_2003,
	title = {From genome to enzyme: {Analysis} of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium {Synechocystis} sp {PCC} 6803},
	volume = {44},
	doi = {Doi 10.1093/Pcp/Pcg086},
	abstract = {Activities of glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, phosphofructokinase (PFK), enolase, pyruvate kinase (PK) and phosphoenolpyruvate (PEP) carboxylase were determined in extracts of photoautotrophic, mixotrophic, and heterotrophic cultures of Synechocystis sp. PCC 6803. Annotated genomes of Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 were analyzed for the respective predicted physical properties of each enzyme investigated here. Enzymatic activity was largely unaffected by nutritional mode, with the exception of glucokinase and PK whose activities were significantly elevated in heterotrophic cultures of Synechocystis sp. PCC 6803. PFK activity was insensitive to bacterial PFK-A (allosteric) effectors such as PEP, implying that Synechocystis PFK should be classified as a PFK-B (non-allosteric). Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated).},
	language = {English},
	number = {7},
	journal = {Plant and Cell Physiology},
	author = {Knowles, V L and Plaxton, W C},
	year = {2003},
	keywords = {anabaena, cyanobacteria, escherichia-coli, genes, genome sequence, glycolysis, metabolism, oxidative pentose-phosphate pathway synechocystis, phosphoenolpyruvate carboxylase, purification, pyruvate-kinase},
	pages = {758--763},
}

{"_id":"KPefL6yAmF3MwY9T3","bibbaseid":"knowles-plaxton-fromgenometoenzymeanalysisofkeyglycolyticandoxidativepentosephosphatepathwayenzymesinthecyanobacteriumsynechocystissppcc6803-2003","author_short":["Knowles, V L","Plaxton, W C"],"bibdata":{"bibtype":"article","type":"article","title":"From genome to enzyme: Analysis of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium Synechocystis sp PCC 6803","volume":"44","doi":"Doi 10.1093/Pcp/Pcg086","abstract":"Activities of glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, phosphofructokinase (PFK), enolase, pyruvate kinase (PK) and phosphoenolpyruvate (PEP) carboxylase were determined in extracts of photoautotrophic, mixotrophic, and heterotrophic cultures of Synechocystis sp. PCC 6803. Annotated genomes of Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 were analyzed for the respective predicted physical properties of each enzyme investigated here. Enzymatic activity was largely unaffected by nutritional mode, with the exception of glucokinase and PK whose activities were significantly elevated in heterotrophic cultures of Synechocystis sp. PCC 6803. PFK activity was insensitive to bacterial PFK-A (allosteric) effectors such as PEP, implying that Synechocystis PFK should be classified as a PFK-B (non-allosteric). Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated).","language":"English","number":"7","journal":"Plant and Cell Physiology","author":[{"propositions":[],"lastnames":["Knowles"],"firstnames":["V","L"],"suffixes":[]},{"propositions":[],"lastnames":["Plaxton"],"firstnames":["W","C"],"suffixes":[]}],"year":"2003","keywords":"anabaena, cyanobacteria, escherichia-coli, genes, genome sequence, glycolysis, metabolism, oxidative pentose-phosphate pathway synechocystis, phosphoenolpyruvate carboxylase, purification, pyruvate-kinase","pages":"758–763","bibtex":"@article{knowles_genome_2003,\n\ttitle = {From genome to enzyme: {Analysis} of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium {Synechocystis} sp {PCC} 6803},\n\tvolume = {44},\n\tdoi = {Doi 10.1093/Pcp/Pcg086},\n\tabstract = {Activities of glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, phosphofructokinase (PFK), enolase, pyruvate kinase (PK) and phosphoenolpyruvate (PEP) carboxylase were determined in extracts of photoautotrophic, mixotrophic, and heterotrophic cultures of Synechocystis sp. PCC 6803. Annotated genomes of Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 were analyzed for the respective predicted physical properties of each enzyme investigated here. Enzymatic activity was largely unaffected by nutritional mode, with the exception of glucokinase and PK whose activities were significantly elevated in heterotrophic cultures of Synechocystis sp. PCC 6803. PFK activity was insensitive to bacterial PFK-A (allosteric) effectors such as PEP, implying that Synechocystis PFK should be classified as a PFK-B (non-allosteric). Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated).},\n\tlanguage = {English},\n\tnumber = {7},\n\tjournal = {Plant and Cell Physiology},\n\tauthor = {Knowles, V L and Plaxton, W C},\n\tyear = {2003},\n\tkeywords = {anabaena, cyanobacteria, escherichia-coli, genes, genome sequence, glycolysis, metabolism, oxidative pentose-phosphate pathway synechocystis, phosphoenolpyruvate carboxylase, purification, pyruvate-kinase},\n\tpages = {758--763},\n}\n\n","author_short":["Knowles, V L","Plaxton, W C"],"key":"knowles_genome_2003","id":"knowles_genome_2003","bibbaseid":"knowles-plaxton-fromgenometoenzymeanalysisofkeyglycolyticandoxidativepentosephosphatepathwayenzymesinthecyanobacteriumsynechocystissppcc6803-2003","role":"author","urls":{},"keyword":["anabaena","cyanobacteria","escherichia-coli","genes","genome sequence","glycolysis","metabolism","oxidative pentose-phosphate pathway synechocystis","phosphoenolpyruvate carboxylase","purification","pyruvate-kinase"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/adrienthurotte","dataSources":["MQg4y2ke6Pj8nXrzF"],"keywords":["anabaena","cyanobacteria","escherichia-coli","genes","genome sequence","glycolysis","metabolism","oxidative pentose-phosphate pathway synechocystis","phosphoenolpyruvate carboxylase","purification","pyruvate-kinase"],"search_terms":["genome","enzyme","analysis","key","glycolytic","oxidative","pentose","phosphate","pathway","enzymes","cyanobacterium","synechocystis","pcc","6803","knowles","plaxton"],"title":"From genome to enzyme: Analysis of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium Synechocystis sp PCC 6803","year":2003}