Three-dimensional imaging of xylem at cell wall level through near field nano holotomography. Koddenberg, T., Greving, I., Hagemann, J., Flenner, S., Krause, A., Laipple, D., Klein, K. C., Schmitt, U., Schuster, M., Wolf, A., Seifert, M., Ludwig, V., Funk, S., Militz, H., & Nopens, M. Scientific Reports, 11(1):4574, February, 2021. Number: 1 Publisher: Nature Publishing Group
Three-dimensional imaging of xylem at cell wall level through near field nano holotomography [link]Paper  doi  abstract   bibtex   
Detailed imaging of the three-dimensionally complex architecture of xylary plants is important for studying biological and mechanical functions of woody plants. Apart from common two-dimensional microscopy, X-ray micro-computed tomography has been established as a three-dimensional (3D) imaging method for studying the hydraulic function of wooden plants. However, this X-ray imaging method can barely reach the resolution needed to see the minute structures (e.g. pit membrane). To complement the xylem structure with 3D views at the nanoscale level, X-ray near-field nano-holotomography (NFH) was applied to analyze the wood species Pinus sylvestris and Fagus sylvatica. The demanded small specimens required focused ion beam (FIB) application. The FIB milling, however, influenced the image quality through gallium implantation on the cell-wall surfaces. The measurements indicated that NFH is appropriate for imaging wood at nanometric resolution. With a 26 nm voxel pitch, the structure of the cell-wall surface in Pinus sylvestris could be visualized in genuine detail. In wood of Fagus sylvatica, the structure of a pit pair, including the pit membrane, between two neighboring fibrous cells could be traced tomographically.
@article{koddenberg_three-dimensional_2021,
	title = {Three-dimensional imaging of xylem at cell wall level through near field nano holotomography},
	volume = {11},
	copyright = {2021 The Author(s)},
	issn = {2045-2322},
	url = {https://www.nature.com/articles/s41598-021-83885-8},
	doi = {10.1038/s41598-021-83885-8},
	abstract = {Detailed imaging of the three-dimensionally complex architecture of xylary plants is important for studying biological and mechanical functions of woody plants. Apart from common two-dimensional microscopy, X-ray micro-computed tomography has been established as a three-dimensional (3D) imaging method for studying the hydraulic function of wooden plants. However, this X-ray imaging method can barely reach the resolution needed to see the minute structures (e.g. pit membrane). To complement the xylem structure with 3D views at the nanoscale level, X-ray near-field nano-holotomography (NFH) was applied to analyze the wood species Pinus sylvestris and Fagus sylvatica. The demanded small specimens required focused ion beam (FIB) application. The FIB milling, however, influenced the image quality through gallium implantation on the cell-wall surfaces. The measurements indicated that NFH is appropriate for imaging wood at nanometric resolution. With a 26 nm voxel pitch, the structure of the cell-wall surface in Pinus sylvestris could be visualized in genuine detail. In wood of Fagus sylvatica, the structure of a pit pair, including the pit membrane, between two neighboring fibrous cells could be traced tomographically.},
	language = {en},
	number = {1},
	urldate = {2021-02-25},
	journal = {Scientific Reports},
	author = {Koddenberg, Tim and Greving, Imke and Hagemann, Johannes and Flenner, Silja and Krause, Andreas and Laipple, Daniel and Klein, Kim C. and Schmitt, Uwe and Schuster, Max and Wolf, Andreas and Seifert, Maria and Ludwig, Veronika and Funk, Stefan and Militz, Holger and Nopens, Martin},
	month = feb,
	year = {2021},
	note = {Number: 1
Publisher: Nature Publishing Group},
	pages = {4574},
	file = {Full Text PDF:C\:\\Users\\Eva\\Zotero\\storage\\RKLG3KJE\\Koddenberg et al. - 2021 - Three-dimensional imaging of xylem at cell wall le.pdf:application/pdf;Snapshot:C\:\\Users\\Eva\\Zotero\\storage\\ZWEWZCCI\\s41598-021-83885-8.html:text/html},
}

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