Ultrafast tissue staining with chemical tags

Ultrafast tissue staining with chemical tags. Kohl, J., Ng, J., Cachero, S., Ciabatti, E., Dolan, M., Sutcliffe, B., Tozer, A., Ruehle, S., Krueger, D., Frechter, S., Branco, T., Tripodi, M., & Jefferis, G. S. X. E. Proceedings of the National Academy of Sciences, August, 2014.

Paper doi abstract bibtex

Genetically encoded fluorescent proteins and immunostaining are widely used to detect cellular and subcellular structures in fixed biological samples. However, for thick or whole-mount tissue, each approach suffers from limitations, including limited spectral flexibility and lower signal or slow speed, poor penetration, and high background labeling, respectively. We have overcome these limitations by using transgenically expressed chemical tags for rapid, even, high-signal and low-background labeling of thick biological tissues. We first construct a platform of widely applicable transgenic Drosophila reporter lines, demonstrating that chemical labeling can accelerate staining of whole-mount fly brains by a factor of 100. Using viral vectors to deliver chemical tags into the mouse brain, we then demonstrate that this labeling strategy works well in mice. Thus this tag-based approach drastically improves the speed and specificity of labeling genetically marked cells in intact and/or thick biological samples.

@article{kohl_ultrafast_2014,
	title = {Ultrafast tissue staining with chemical tags},
	issn = {0027-8424, 1091-6490},
	url = {http://www.pnas.org/content/early/2014/08/27/1411087111},
	doi = {10.1073/pnas.1411087111},
	abstract = {Genetically encoded fluorescent proteins and immunostaining are widely used to detect cellular and subcellular structures in fixed biological samples. However, for thick or whole-mount tissue, each approach suffers from limitations, including limited spectral flexibility and lower signal or slow speed, poor penetration, and high background labeling, respectively. We have overcome these limitations by using transgenically expressed chemical tags for rapid, even, high-signal and low-background labeling of thick biological tissues. We first construct a platform of widely applicable transgenic Drosophila reporter lines, demonstrating that chemical labeling can accelerate staining of whole-mount fly brains by a factor of 100. Using viral vectors to deliver chemical tags into the mouse brain, we then demonstrate that this labeling strategy works well in mice. Thus this tag-based approach drastically improves the speed and specificity of labeling genetically marked cells in intact and/or thick biological samples.},
	language = {en},
	urldate = {2014-09-01TZ},
	journal = {Proceedings of the National Academy of Sciences},
	author = {Kohl, Johannes and Ng, Julian and Cachero, Sebastian and Ciabatti, Ernesto and Dolan, Michael-John and Sutcliffe, Ben and Tozer, Adam and Ruehle, Sabine and Krueger, Daniel and Frechter, Shahar and Branco, Tiago and Tripodi, Marco and Jefferis, Gregory S. X. E.},
	month = aug,
	year = {2014},
	pmid = {25157152},
	pages = {201411087}
}

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