@article{kopecny_probing_2006,
	title = {Probing cytokinin homeostasis in {Arabidopsis} thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene},
	volume = {171},
	issn = {0168-9452},
	doi = {10/d8cm9d},
	abstract = {Engineering transgenic plants with reduced cytokinin (Ck) contents is away to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described. We report on Arabidopsis transgenics constitutively overexpressing the secreted native form or an engineered non-secreted form of the maize CKO1{\textbackslash} enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the non-secreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. AtIPT genes involved in Ck biosynthesis were found to be up-regulated in those transgenics while all AtCKO genes were down-regulated except At5g21482, coding for a putative cytoplasmic enzyme. Cytokinin deficiency in transgenics was not counter-balanced by a higher sensitivity: expression of a cytokinin receptor and type-A response regulators was decreased as well as plant response to benzyladenine. (c) 2006 Elsevier Ireland Ltd. All rights reserved.},
	language = {English},
	number = {1},
	journal = {Plant Science},
	author = {Kopecny, David and Tarkowski, Petr and Majira, Amel and Bouchez-Mahiout, Isabelle and Nogue, Fabien and Lauriere, Michel and Sandberg, Goran and Laloue, Michel and Houba-Herin, Nicole},
	month = jul,
	year = {2006},
	note = {Place: Clare
Publisher: Elsevier Ireland Ltd
WOS:000238454800013},
	keywords = {Arabidopsis thaliana, biosynthesis, cytokinin oxidase/dehydrogenase, derivatization, expression, homeostasis, maize, o-glucosyltransferase, overexpresser, oxidase, oxidase/dehydrogenase, plants, response regulator, root-meristem, zea-mays},
	pages = {114--122},
}

{"_id":"zvuMFjAs3jQPdYjNv","bibbaseid":"kopecny-tarkowski-majira-bouchezmahiout-nogue-lauriere-sandberg-laloue-etal-probingcytokininhomeostasisinarabidopsisthalianabyconstitutivelyoverexpressingtwoformsofthemaizecytokininoxidasedehydrogenase1gene-2006","author_short":["Kopecny, D.","Tarkowski, P.","Majira, A.","Bouchez-Mahiout, I.","Nogue, F.","Lauriere, M.","Sandberg, G.","Laloue, M.","Houba-Herin, N."],"bibdata":{"bibtype":"article","type":"article","title":"Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene","volume":"171","issn":"0168-9452","doi":"10/d8cm9d","abstract":"Engineering transgenic plants with reduced cytokinin (Ck) contents is away to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described. We report on Arabidopsis transgenics constitutively overexpressing the secreted native form or an engineered non-secreted form of the maize CKO1\\ enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the non-secreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. AtIPT genes involved in Ck biosynthesis were found to be up-regulated in those transgenics while all AtCKO genes were down-regulated except At5g21482, coding for a putative cytoplasmic enzyme. Cytokinin deficiency in transgenics was not counter-balanced by a higher sensitivity: expression of a cytokinin receptor and type-A response regulators was decreased as well as plant response to benzyladenine. (c) 2006 Elsevier Ireland Ltd. All rights reserved.","language":"English","number":"1","journal":"Plant Science","author":[{"propositions":[],"lastnames":["Kopecny"],"firstnames":["David"],"suffixes":[]},{"propositions":[],"lastnames":["Tarkowski"],"firstnames":["Petr"],"suffixes":[]},{"propositions":[],"lastnames":["Majira"],"firstnames":["Amel"],"suffixes":[]},{"propositions":[],"lastnames":["Bouchez-Mahiout"],"firstnames":["Isabelle"],"suffixes":[]},{"propositions":[],"lastnames":["Nogue"],"firstnames":["Fabien"],"suffixes":[]},{"propositions":[],"lastnames":["Lauriere"],"firstnames":["Michel"],"suffixes":[]},{"propositions":[],"lastnames":["Sandberg"],"firstnames":["Goran"],"suffixes":[]},{"propositions":[],"lastnames":["Laloue"],"firstnames":["Michel"],"suffixes":[]},{"propositions":[],"lastnames":["Houba-Herin"],"firstnames":["Nicole"],"suffixes":[]}],"month":"July","year":"2006","note":"Place: Clare Publisher: Elsevier Ireland Ltd WOS:000238454800013","keywords":"Arabidopsis thaliana, biosynthesis, cytokinin oxidase/dehydrogenase, derivatization, expression, homeostasis, maize, o-glucosyltransferase, overexpresser, oxidase, oxidase/dehydrogenase, plants, response regulator, root-meristem, zea-mays","pages":"114–122","bibtex":"@article{kopecny_probing_2006,\n\ttitle = {Probing cytokinin homeostasis in {Arabidopsis} thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene},\n\tvolume = {171},\n\tissn = {0168-9452},\n\tdoi = {10/d8cm9d},\n\tabstract = {Engineering transgenic plants with reduced cytokinin (Ck) contents is away to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described. We report on Arabidopsis transgenics constitutively overexpressing the secreted native form or an engineered non-secreted form of the maize CKO1{\\textbackslash} enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the non-secreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. AtIPT genes involved in Ck biosynthesis were found to be up-regulated in those transgenics while all AtCKO genes were down-regulated except At5g21482, coding for a putative cytoplasmic enzyme. Cytokinin deficiency in transgenics was not counter-balanced by a higher sensitivity: expression of a cytokinin receptor and type-A response regulators was decreased as well as plant response to benzyladenine. (c) 2006 Elsevier Ireland Ltd. All rights reserved.},\n\tlanguage = {English},\n\tnumber = {1},\n\tjournal = {Plant Science},\n\tauthor = {Kopecny, David and Tarkowski, Petr and Majira, Amel and Bouchez-Mahiout, Isabelle and Nogue, Fabien and Lauriere, Michel and Sandberg, Goran and Laloue, Michel and Houba-Herin, Nicole},\n\tmonth = jul,\n\tyear = {2006},\n\tnote = {Place: Clare\nPublisher: Elsevier Ireland Ltd\nWOS:000238454800013},\n\tkeywords = {Arabidopsis thaliana, biosynthesis, cytokinin oxidase/dehydrogenase, derivatization, expression, homeostasis, maize, o-glucosyltransferase, overexpresser, oxidase, oxidase/dehydrogenase, plants, response regulator, root-meristem, zea-mays},\n\tpages = {114--122},\n}\n\n\n\n","author_short":["Kopecny, D.","Tarkowski, P.","Majira, A.","Bouchez-Mahiout, I.","Nogue, F.","Lauriere, M.","Sandberg, G.","Laloue, M.","Houba-Herin, N."],"key":"kopecny_probing_2006","id":"kopecny_probing_2006","bibbaseid":"kopecny-tarkowski-majira-bouchezmahiout-nogue-lauriere-sandberg-laloue-etal-probingcytokininhomeostasisinarabidopsisthalianabyconstitutivelyoverexpressingtwoformsofthemaizecytokininoxidasedehydrogenase1gene-2006","role":"author","urls":{},"keyword":["Arabidopsis thaliana","biosynthesis","cytokinin oxidase/dehydrogenase","derivatization","expression","homeostasis","maize","o-glucosyltransferase","overexpresser","oxidase","oxidase/dehydrogenase","plants","response regulator","root-meristem","zea-mays"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs","fvfkWcShg3Mybjoog","Tu3jPdZyJF3j547xT","3zTPPmKj8BiTcpc6C"],"keywords":["arabidopsis thaliana","biosynthesis","cytokinin oxidase/dehydrogenase","derivatization","expression","homeostasis","maize","o-glucosyltransferase","overexpresser","oxidase","oxidase/dehydrogenase","plants","response regulator","root-meristem","zea-mays"],"search_terms":["probing","cytokinin","homeostasis","arabidopsis","thaliana","constitutively","overexpressing","two","forms","maize","cytokinin","oxidase","dehydrogenase","gene","kopecny","tarkowski","majira","bouchez-mahiout","nogue","lauriere","sandberg","laloue","houba-herin"],"title":"Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene","year":2006}