The saccharomyces cerevisiae yeast strain for testing environmental mutagens based on the interaction between rad2 and himl mutations. Kovaptsova, S. & Korolev, V. Genetika, 32(3):366-372, 1996. cited By 7
The saccharomyces cerevisiae yeast strain for testing environmental mutagens based on the interaction between rad2 and himl mutations [link]Paper  abstract   bibtex   
The interaction between mutations at the RAD2 and HIM1 genes was studied. The RAD2 gene encodes endonuclease involved in nucleotide excision repair. Mutants at this gene are highly sensitive to the lethal effect of a variety of mutagens. The product of the ///A/7 gene is needed for correction of mismatched bases and repair of premutational DN A damage. Mutations in this gene lead to the formation of the mutator phenotype and high sensitivity to induced mutagenesis. The double rad2 himl mutant manifested the synergic type of interaction. The level of UV-induced mutagenesis in the double mutant was five times higher than in single mutants, and the absolute yield of forward mutations in five genes controlling adenine biosynthesis was 1 to 2%. UV-induced mutagenesis was increased, at low doses, by several orders of magnitude in the double mutant, compared to the wild-type strain. The high level of mutagenesis in this mutant was caused by ethyl and methyl methanesulfonate. These properties of the stock with the double rad.2 himl mutation makes it promising as a tester in analysis of the gene toxicity of different substances.
@ARTICLE{Kovaptsova1996366,
author={Kovaptsova, S.B. and Korolev, V.G.},
title={The saccharomyces cerevisiae yeast strain for testing environmental mutagens based on the interaction between rad2 and himl mutations},
journal={Genetika},
year={1996},
volume={32},
number={3},
pages={366-372},
note={cited By 7},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030101572&partnerID=40&md5=37a68d4064520877dacabbe978d1cec2},
affiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},
abstract={The interaction between mutations at the RAD2 and HIM1 genes was studied. The RAD2 gene encodes endonuclease involved in nucleotide excision repair. Mutants at this gene are highly sensitive to the lethal effect of a variety of mutagens. The product of the ///A/7 gene is needed for correction of mismatched bases and repair of premutational DN A damage. Mutations in this gene lead to the formation of the mutator phenotype and high sensitivity to induced mutagenesis. The double rad2 himl mutant manifested the synergic type of interaction. The level of UV-induced mutagenesis in the double mutant was five times higher than in single mutants, and the absolute yield of forward mutations in five genes controlling adenine biosynthesis was 1 to 2%. UV-induced mutagenesis was increased, at low doses, by several orders of magnitude in the double mutant, compared to the wild-type strain. The high level of mutagenesis in this mutant was caused by ethyl and methyl methanesulfonate. These properties of the stock with the double rad.2 himl mutation makes it promising as a tester in analysis of the gene toxicity of different substances.},
correspondence_address1={Kovaptsova, S.B.; Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},
issn={00166758},
coden={GNKAA},
pubmed_id={8723629},
language={Russian},
abbrev_source_title={Genetika},
document_type={Article},
source={Scopus},
}

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