Infection pre-Ad26.COV2.S-vaccination primes greater class switching and reduced CXCR5 expression by SARS-CoV-2-specific memory B cells. Krause, R. G E, Moyo-Gwete, T., Richardson, S. I, Makhado, Z., Manamela, N. P, Hermanus, T., Mkhize, N. N, Keeton, R., Benede, N., Mennen, M., Skelem, S., Karim, F., Khan, K., Riou, C., Ntusi, N. A B, Goga, A., Gray, G., Hanekom, W., Garrett, N., Bekker, L., Groll, A., Sigal, A., Moore, P. L, Burgers, W. A, & Leslie, A. npj Vaccines, 8(1):119, Nature Publishing Group, aug, 2023. ![Infection pre-Ad26.COV2.S-vaccination primes greater class switching and reduced CXCR5 expression by SARS-CoV-2-specific memory B cells [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Neutralizing antibodies strongly correlate with protection for COVID-19 vaccines, but the corresponding memory B cells that form to protect against future infection are relatively understudied. Here we examine the effect of prior SARS-CoV-2 infection on the magnitude and phenotype of the memory B cell response to single dose Johnson and Johnson (Ad26.COV2.S) vaccination in South African health care workers. Participants were either naïve to SARS-CoV-2 or had been infected before vaccination. SARS-CoV-2-specific memory B-cells expand in response to Ad26.COV2.S and are maintained for the study duration (84 days) in all individuals. However, prior infection is associated with a greater frequency of these cells, a significant reduction in expression of the germinal center chemokine receptor CXCR5, and increased class switching. These B cell features correlated with neutralization and antibody-dependent cytotoxicity (ADCC) activity, and with the frequency of SARS-CoV-2 specific circulating T follicular helper cells (cTfh). Vaccination-induced effective neutralization of the D614G variant in both infected and naïve participants but boosted neutralizing antibodies against the Beta and Omicron variants only in participants with prior infection. In addition, the SARS-CoV-2 specific CD8+ T cell response correlated with increased memory B cell expression of the lung-homing receptor CXCR3, which was sustained in the previously infected group. Finally, although vaccination achieved equivalent B cell activation regardless of infection history, it was negatively impacted by age. These data show that phenotyping the response to vaccination can provide insight into the impact of prior infection on memory B cell homing, CSM, cTfh, and neutralization activity. These data can provide early signals to inform studies of vaccine boosting, durability, and co-morbidities.
@article{Krause2023,
abstract = {Neutralizing antibodies strongly correlate with protection for COVID-19 vaccines, but the corresponding memory B cells that form to protect against future infection are relatively understudied. Here we examine the effect of prior SARS-CoV-2 infection on the magnitude and phenotype of the memory B cell response to single dose Johnson and Johnson (Ad26.COV2.S) vaccination in South African health care workers. Participants were either na{\"{i}}ve to SARS-CoV-2 or had been infected before vaccination. SARS-CoV-2-specific memory B-cells expand in response to Ad26.COV2.S and are maintained for the study duration (84 days) in all individuals. However, prior infection is associated with a greater frequency of these cells, a significant reduction in expression of the germinal center chemokine receptor CXCR5, and increased class switching. These B cell features correlated with neutralization and antibody-dependent cytotoxicity (ADCC) activity, and with the frequency of SARS-CoV-2 specific circulating T follicular helper cells (cTfh). Vaccination-induced effective neutralization of the D614G variant in both infected and na{\"{i}}ve participants but boosted neutralizing antibodies against the Beta and Omicron variants only in participants with prior infection. In addition, the SARS-CoV-2 specific CD8+ T cell response correlated with increased memory B cell expression of the lung-homing receptor CXCR3, which was sustained in the previously infected group. Finally, although vaccination achieved equivalent B cell activation regardless of infection history, it was negatively impacted by age. These data show that phenotyping the response to vaccination can provide insight into the impact of prior infection on memory B cell homing, CSM, cTfh, and neutralization activity. These data can provide early signals to inform studies of vaccine boosting, durability, and co-morbidities.},
author = {Krause, Robert G E and Moyo-Gwete, Thandeka and Richardson, Simone I and Makhado, Zanele and Manamela, Nelia P and Hermanus, Tandile and Mkhize, Nonhlanhla N and Keeton, Roanne and Benede, Ntombi and Mennen, Mathilda and Skelem, Sango and Karim, Farina and Khan, Khadija and Riou, Catherine and Ntusi, Ntobeko A B and Goga, Ameena and Gray, Glenda and Hanekom, Willem and Garrett, Nigel and Bekker, Linda-Gail and Groll, Andreas and Sigal, Alex and Moore, Penny L and Burgers, Wendy A and Leslie, Alasdair},
doi = {10.1038/s41541-023-00724-9},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Krause et al. - 2023 - Infection pre-Ad26.COV2.S-vaccination primes greater class switching and reduced CXCR5 expression by SARS-CoV-2-s.pdf:pdf},
issn = {2059-0105},
journal = {npj Vaccines},
keywords = {DNA vaccines,OA,OA{\_}PMC,Viral infection,fund{\_}ack,original},
mendeley-tags = {OA,OA{\_}PMC,fund{\_}ack,original},
month = {aug},
number = {1},
pages = {119},
pmid = {37573434},
publisher = {Nature Publishing Group},
title = {{Infection pre-Ad26.COV2.S-vaccination primes greater class switching and reduced CXCR5 expression by SARS-CoV-2-specific memory B cells}},
url = {https://www.nature.com/articles/s41541-023-00724-9},
volume = {8},
year = {2023}
}
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