@article{Krause2022,
abstract = {Neutralizing antibodies strongly correlate with protection for COVID-19 vaccines, but the corresponding memory B cells that form to protect against future infection are relatively understudied. Here we examine the effect of prior SARS-CoV-2 infection on the magnitude and phenotype of the B cell response to single dose Johnson and Johnson (Ad26.COV2.S) vaccination in South African health care workers. SARS-CoV- 2 speci?c memory responses expand in response to Ad26.COV2.S and are maintained for the study duration (84 days) in all individuals. However, prior infection is associated with a greater frequency of these cells, a more prominent germinal center (GC) response, and increased class switched memory (CSM). These B cell features correlated with both neutralization and antibody-dependent cytotoxicity (ADCC) activity, and with the frequency of SARS-CoV-2 speci?c circulating T follicular helper cells (cTfh). In addition, the SARS-CoV-2 speci?c CD8+ T cell response correlated with increased memory B cell lung- homing, which was sustained in the infected group. Finally, although vaccination achieved equivalent B cell activation regardless of infection history, it was negatively impacted by age. These data show that phenotyping the B cell response to vaccination can provide mechanistic insight into the impact of prior infection on GC homing, CSM, cTfh, and neutralization activity. These data can provide early signals and mechanistic understanding to inform studies of vaccine boosting, durability, and co-morbidities.},
author = {Krause, Rob and Richardson, Simone and Makhado, Zanele and Manamela, Nelia and Hermanus, Tandile and Mkhize, Nono and Keeton, Roanne and Benede, Ntombi and Mennen, Mathilda and Riou, Catherine},
doi = {10.21203/RS.3.RS-1170883/V1},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Krause et al. - 2022 - SARS-CoV-2 specific B cell memory drives improved class switching and tissue homing responses to single dose Ad26.pdf:pdf},
journal = {Research Square},
keywords = {OA,fund{\_}ack,original},
mendeley-tags = {OA,fund{\_}ack,original},
month = {jan},
pages = {10.21203/rs.3.rs--1170883/v1},
title = {{SARS-CoV-2 specific B cell memory drives improved class switching and tissue homing responses to single dose Ad26.COV2.S vaccination in previously infected recipients}},
url = {https://www.researchsquare.com https://www.researchsquare.com/article/rs-1170883/v1},
year = {2022}
}

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SARS-CoV- 2 speci?c memory responses expand in response to Ad26.COV2.S and are maintained for the study duration (84 days) in all individuals. However, prior infection is associated with a greater frequency of these cells, a more prominent germinal center (GC) response, and increased class switched memory (CSM). These B cell features correlated with both neutralization and antibody-dependent cytotoxicity (ADCC) activity, and with the frequency of SARS-CoV-2 speci?c circulating T follicular helper cells (cTfh). In addition, the SARS-CoV-2 speci?c CD8+ T cell response correlated with increased memory B cell lung- homing, which was sustained in the infected group. Finally, although vaccination achieved equivalent B cell activation regardless of infection history, it was negatively impacted by age. These data show that phenotyping the B cell response to vaccination can provide mechanistic insight into the impact of prior infection on GC homing, CSM, cTfh, and neutralization activity. 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