Monoclonal antibodies to the turtle cortex reveal neuronal subsets, antigenic cross-reactivity with the mammalian neocortex, and forebrain structures sharing a pallial derivation. Kriegstein, A R, Shen, J M, & Eshhar, N J Comp Neurol, 254(3):330–340, United States, December, 1986. abstract bibtex The dorsal cortex of the pond turtle (Pseudemys scripta) is a relatively simple structure consisting of two principal classes of neurons that occupy three distinct layers. Morphological, pharmacological, and physiological data suggest many similarities to the mammalian neocortex, rendering it an interesting preparation for comparative studies. We prepared monoclonal antibodies to the turtle dorsal cortex by immunizing mice with cortical tissue from adult turtles. Twelve antibodies were generated that recognize specific components of the turtle cortex. Among these, eight antibodies label only neurons and four label only ependymal glial cells. Differences in tissue staining pattern and immunoglobulin class suggest a heterogeneity of antigenic specificity among the antibodies. The staining patterns of three of our antibodies are described. TC3, like all other neuron-marking antibodies generated, labels a subset of both pyramidal and stellate cell types. It also cross-reacts with a subset of mammalian cortical neurons and labels them with a pattern similar to that observed in the turtle cortex. TC5 stains ependymal cells and their glial processes in the turtle cortex, and cross-reacts with fibrous astrocytelike processes in mammalian neocortical white matter. TC9 appears to recognize antigens of neurons sharing a pallial derivation in turtle.
@ARTICLE{Kriegstein1986-xj,
title = "Monoclonal antibodies to the turtle cortex reveal neuronal
subsets, antigenic cross-reactivity with the mammalian neocortex,
and forebrain structures sharing a pallial derivation",
author = "Kriegstein, A R and Shen, J M and Eshhar, N",
abstract = "The dorsal cortex of the pond turtle (Pseudemys scripta) is a
relatively simple structure consisting of two principal classes
of neurons that occupy three distinct layers. Morphological,
pharmacological, and physiological data suggest many similarities
to the mammalian neocortex, rendering it an interesting
preparation for comparative studies. We prepared monoclonal
antibodies to the turtle dorsal cortex by immunizing mice with
cortical tissue from adult turtles. Twelve antibodies were
generated that recognize specific components of the turtle
cortex. Among these, eight antibodies label only neurons and four
label only ependymal glial cells. Differences in tissue staining
pattern and immunoglobulin class suggest a heterogeneity of
antigenic specificity among the antibodies. The staining patterns
of three of our antibodies are described. TC3, like all other
neuron-marking antibodies generated, labels a subset of both
pyramidal and stellate cell types. It also cross-reacts with a
subset of mammalian cortical neurons and labels them with a
pattern similar to that observed in the turtle cortex. TC5 stains
ependymal cells and their glial processes in the turtle cortex,
and cross-reacts with fibrous astrocytelike processes in
mammalian neocortical white matter. TC9 appears to recognize
antigens of neurons sharing a pallial derivation in turtle.",
journal = "J Comp Neurol",
volume = 254,
number = 3,
pages = "330--340",
month = dec,
year = 1986,
address = "United States",
language = "en"
}
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Morphological, pharmacological, and physiological data suggest many similarities to the mammalian neocortex, rendering it an interesting preparation for comparative studies. We prepared monoclonal antibodies to the turtle dorsal cortex by immunizing mice with cortical tissue from adult turtles. Twelve antibodies were generated that recognize specific components of the turtle cortex. Among these, eight antibodies label only neurons and four label only ependymal glial cells. Differences in tissue staining pattern and immunoglobulin class suggest a heterogeneity of antigenic specificity among the antibodies. The staining patterns of three of our antibodies are described. TC3, like all other neuron-marking antibodies generated, labels a subset of both pyramidal and stellate cell types. It also cross-reacts with a subset of mammalian cortical neurons and labels them with a pattern similar to that observed in the turtle cortex. TC5 stains ependymal cells and their glial processes in the turtle cortex, and cross-reacts with fibrous astrocytelike processes in mammalian neocortical white matter. TC9 appears to recognize antigens of neurons sharing a pallial derivation in turtle.","journal":"J Comp Neurol","volume":"254","number":"3","pages":"330–340","month":"December","year":"1986","address":"United States","language":"en","bibtex":"@ARTICLE{Kriegstein1986-xj,\n title = \"Monoclonal antibodies to the turtle cortex reveal neuronal\n subsets, antigenic cross-reactivity with the mammalian neocortex,\n and forebrain structures sharing a pallial derivation\",\n author = \"Kriegstein, A R and Shen, J M and Eshhar, N\",\n abstract = \"The dorsal cortex of the pond turtle (Pseudemys scripta) is a\n relatively simple structure consisting of two principal classes\n of neurons that occupy three distinct layers. Morphological,\n pharmacological, and physiological data suggest many similarities\n to the mammalian neocortex, rendering it an interesting\n preparation for comparative studies. We prepared monoclonal\n antibodies to the turtle dorsal cortex by immunizing mice with\n cortical tissue from adult turtles. Twelve antibodies were\n generated that recognize specific components of the turtle\n cortex. Among these, eight antibodies label only neurons and four\n label only ependymal glial cells. Differences in tissue staining\n pattern and immunoglobulin class suggest a heterogeneity of\n antigenic specificity among the antibodies. The staining patterns\n of three of our antibodies are described. TC3, like all other\n neuron-marking antibodies generated, labels a subset of both\n pyramidal and stellate cell types. It also cross-reacts with a\n subset of mammalian cortical neurons and labels them with a\n pattern similar to that observed in the turtle cortex. TC5 stains\n ependymal cells and their glial processes in the turtle cortex,\n and cross-reacts with fibrous astrocytelike processes in\n mammalian neocortical white matter. TC9 appears to recognize\n antigens of neurons sharing a pallial derivation in turtle.\",\n journal = \"J Comp Neurol\",\n volume = 254,\n number = 3,\n pages = \"330--340\",\n month = dec,\n year = 1986,\n address = \"United States\",\n language = \"en\"\n}\n","author_short":["Kriegstein, A R","Shen, J M","Eshhar, N"],"key":"Kriegstein1986-xj","id":"Kriegstein1986-xj","bibbaseid":"kriegstein-shen-eshhar-monoclonalantibodiestotheturtlecortexrevealneuronalsubsetsantigeniccrossreactivitywiththemammalianneocortexandforebrainstructuressharingapallialderivation-1986","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["monoclonal","antibodies","turtle","cortex","reveal","neuronal","subsets","antigenic","cross","reactivity","mammalian","neocortex","forebrain","structures","sharing","pallial","derivation","kriegstein","shen","eshhar"],"title":"Monoclonal antibodies to the turtle cortex reveal neuronal subsets, antigenic cross-reactivity with the mammalian neocortex, and forebrain structures sharing a pallial derivation","year":1986}