Monoclonal antibodies to the turtle cortex reveal neuronal subsets, antigenic cross-reactivity with the mammalian neocortex, and forebrain structures sharing a pallial derivation. Kriegstein, A R, Shen, J M, & Eshhar, N J Comp Neurol, 254(3):330–340, United States, December, 1986.
abstract   bibtex   
The dorsal cortex of the pond turtle (Pseudemys scripta) is a relatively simple structure consisting of two principal classes of neurons that occupy three distinct layers. Morphological, pharmacological, and physiological data suggest many similarities to the mammalian neocortex, rendering it an interesting preparation for comparative studies. We prepared monoclonal antibodies to the turtle dorsal cortex by immunizing mice with cortical tissue from adult turtles. Twelve antibodies were generated that recognize specific components of the turtle cortex. Among these, eight antibodies label only neurons and four label only ependymal glial cells. Differences in tissue staining pattern and immunoglobulin class suggest a heterogeneity of antigenic specificity among the antibodies. The staining patterns of three of our antibodies are described. TC3, like all other neuron-marking antibodies generated, labels a subset of both pyramidal and stellate cell types. It also cross-reacts with a subset of mammalian cortical neurons and labels them with a pattern similar to that observed in the turtle cortex. TC5 stains ependymal cells and their glial processes in the turtle cortex, and cross-reacts with fibrous astrocytelike processes in mammalian neocortical white matter. TC9 appears to recognize antigens of neurons sharing a pallial derivation in turtle.
@ARTICLE{Kriegstein1986-xj,
  title    = "Monoclonal antibodies to the turtle cortex reveal neuronal
              subsets, antigenic cross-reactivity with the mammalian neocortex,
              and forebrain structures sharing a pallial derivation",
  author   = "Kriegstein, A R and Shen, J M and Eshhar, N",
  abstract = "The dorsal cortex of the pond turtle (Pseudemys scripta) is a
              relatively simple structure consisting of two principal classes
              of neurons that occupy three distinct layers. Morphological,
              pharmacological, and physiological data suggest many similarities
              to the mammalian neocortex, rendering it an interesting
              preparation for comparative studies. We prepared monoclonal
              antibodies to the turtle dorsal cortex by immunizing mice with
              cortical tissue from adult turtles. Twelve antibodies were
              generated that recognize specific components of the turtle
              cortex. Among these, eight antibodies label only neurons and four
              label only ependymal glial cells. Differences in tissue staining
              pattern and immunoglobulin class suggest a heterogeneity of
              antigenic specificity among the antibodies. The staining patterns
              of three of our antibodies are described. TC3, like all other
              neuron-marking antibodies generated, labels a subset of both
              pyramidal and stellate cell types. It also cross-reacts with a
              subset of mammalian cortical neurons and labels them with a
              pattern similar to that observed in the turtle cortex. TC5 stains
              ependymal cells and their glial processes in the turtle cortex,
              and cross-reacts with fibrous astrocytelike processes in
              mammalian neocortical white matter. TC9 appears to recognize
              antigens of neurons sharing a pallial derivation in turtle.",
  journal  = "J Comp Neurol",
  volume   =  254,
  number   =  3,
  pages    = "330--340",
  month    =  dec,
  year     =  1986,
  address  = "United States",
  language = "en"
}

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