{"_id":"KnpAsEssTcj4Zooaj","bibbaseid":"kubannek-schrder-krewer-revealingmetabolicstorageprocessesinelectroderespiringbacteriabydifferentialelectrochemicalmassspectrometry-2018","downloads":0,"creationDate":"2018-08-26T14:43:18.177Z","title":"Revealing metabolic storage processes in electrode respiring bacteria by differential electrochemical mass spectrometry","author_short":["Kubannek, F.","Schröder, U.","Krewer, U."],"year":2018,"bibtype":"article","biburl":"https://bibbase.org/zotero/InES_TUBS","bibdata":{"bibtype":"article","type":"article","title":"Revealing metabolic storage processes in electrode respiring bacteria by differential electrochemical mass spectrometry","volume":"121","copyright":"All rights reserved","issn":"1878562X","doi":"10.1016/j.bioelechem.2018.01.014","abstract":"In this work we employ differential electrochemical mass spectrometry (DEMS) in combination with static and dynamic electrochemical techniques for the study of metabolic processes of electrochemically active bacteria. CO2production during acetate oxidation by electrode respiring bacteria was measured, in-vivo and online with a sensitivity of 6.5 ⋅ 10−13mol/s. The correlation of ion current and electrical current provides insight into the interaction of metabolic processes and extra-cellular electron transfer. In low-turnover CVs, two competing potential dependent electron transfer mechanisms were observed and formal potentials of two redox systems that are involved in complete oxidation of acetate to CO2were determined. By balancing charge and carbon flows during dynamic measurements, two significant storage mechanisms in electrochemically active bacteria were identified: 1) a charge storage mechanism that allows substrate oxidation to proceed at a constant rate despite of external current flowing in cathodic direction. 2) a carbon storage mechanism that allows the biofilm to take up acetate at an unchanged rate at very low potentials even though the oxidation to CO2stops. These storage capabilities allow a limited decoupling of electrical current and CO2production rate.","journal":"Bioelectrochemistry","author":[{"propositions":[],"lastnames":["Kubannek"],"firstnames":["F."],"suffixes":[]},{"propositions":[],"lastnames":["Schröder"],"firstnames":["U."],"suffixes":[]},{"propositions":[],"lastnames":["Krewer"],"firstnames":["U."],"suffixes":[]}],"year":"2018","keywords":"Charge and carbon storage, Differential electrochemical mass spectrometry, Mass spectrometric cyclic voltammetry, Microbial fuel cell, Online measurement of CO2production","pages":"160–168","bibtex":"@article{kubannek_revealing_2018,\n\ttitle = {Revealing metabolic storage processes in electrode respiring bacteria by differential electrochemical mass spectrometry},\n\tvolume = {121},\n\tcopyright = {All rights reserved},\n\tissn = {1878562X},\n\tdoi = {10.1016/j.bioelechem.2018.01.014},\n\tabstract = {In this work we employ differential electrochemical mass spectrometry (DEMS) in combination with static and dynamic electrochemical techniques for the study of metabolic processes of electrochemically active bacteria. CO2production during acetate oxidation by electrode respiring bacteria was measured, in-vivo and online with a sensitivity of 6.5 ⋅ 10−13mol/s. The correlation of ion current and electrical current provides insight into the interaction of metabolic processes and extra-cellular electron transfer. In low-turnover CVs, two competing potential dependent electron transfer mechanisms were observed and formal potentials of two redox systems that are involved in complete oxidation of acetate to CO2were determined. By balancing charge and carbon flows during dynamic measurements, two significant storage mechanisms in electrochemically active bacteria were identified: 1) a charge storage mechanism that allows substrate oxidation to proceed at a constant rate despite of external current flowing in cathodic direction. 2) a carbon storage mechanism that allows the biofilm to take up acetate at an unchanged rate at very low potentials even though the oxidation to CO2stops. These storage capabilities allow a limited decoupling of electrical current and CO2production rate.},\n\tjournal = {Bioelectrochemistry},\n\tauthor = {Kubannek, F. and Schröder, U. and Krewer, U.},\n\tyear = {2018},\n\tkeywords = {Charge and carbon storage, Differential electrochemical mass spectrometry, Mass spectrometric cyclic voltammetry, Microbial fuel cell, Online measurement of CO2production},\n\tpages = {160--168},\n}\n\n","author_short":["Kubannek, F.","Schröder, U.","Krewer, U."],"key":"kubannek_revealing_2018","id":"kubannek_revealing_2018","bibbaseid":"kubannek-schrder-krewer-revealingmetabolicstorageprocessesinelectroderespiringbacteriabydifferentialelectrochemicalmassspectrometry-2018","role":"author","urls":{},"keyword":["Charge and carbon storage","Differential electrochemical mass spectrometry","Mass spectrometric cyclic voltammetry","Microbial fuel cell","Online measurement of CO2production"],"metadata":{"authorlinks":{"krewer, u":"https://bibbase.org/show?bib=https%3A%2F%2Fbibbase.org%2Fzotero%2FInES_TUBS&msg=embed"}}},"search_terms":["revealing","metabolic","storage","processes","electrode","respiring","bacteria","differential","electrochemical","mass","spectrometry","kubannek","schröder","krewer"],"keywords":["charge and carbon storage","differential electrochemical mass spectrometry","mass spectrometric cyclic voltammetry","microbial fuel cell","online measurement of co2production"],"authorIDs":["M5XZkuDC9FL7QRGmS"],"dataSources":["67CvPrRggJ3tpQYM9"]}