Impact of storage conditions on the urinary metabolomics fingerprint

Impact of storage conditions on the urinary metabolomics fingerprint. Laparre, J., Kaabia, Z., Mooney, M., Buckley, T., Sherry, M., Le Bizec, B., & Dervilly-Pinel, G. 951:99–107.

Paper doi abstract bibtex

Urine stability during storage is essential in metabolomics to avoid misleading conclusions or erroneous interpretations. Facing the lack of comprehensive studies on urine metabolome stability, the present work performed a follow-up of potential modiﬁcations in urinary chemical proﬁle using LC-HRMS on the basis of two parameters: the storage temperature (þ4 C, À20 C, À80 C and freeze-dried stored at À80 C) and the storage duration (5e144 days). Both HILIC and RP chromatographies have been implemented in order to globally monitor the urinary metabolome. Using an original data processing associated to univariate and multivariate data analysis, our study conﬁrms that chemical proﬁles of urine samples stored at þ4 C are very rapidly modiﬁed, as observed for instance for compounds such as:Nacetyl Glycine, Adenosine, 4-Amino benzoic acid, N-Amino diglycine, creatine, glucuronic acid, 3hydroxy-benzoic acid, pyridoxal, L-pyroglutamic acid, shikimic acid, succinic acid, thymidine, trigonelline and valeryl-carnitine, while it also demonstrates that urine samples stored at À20 C exhibit a global stability over a long period with no major modiﬁcations compared to À80 C condition. This study is the ﬁrst to investigate long term stability of urine samples and report potential modiﬁcations in the urinary metabolome, using both targeted approach monitoring individually a large number (n \textgreater 200) of urinary metabolites and an untargeted strategy enabling assessing for global impact of storage conditions.

@article{laparre_impact_2017,
	title = {Impact of storage conditions on the urinary metabolomics fingerprint},
	volume = {951},
	issn = {00032670},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0003267016314052},
	doi = {10.1016/j.aca.2016.11.055},
	abstract = {Urine stability during storage is essential in metabolomics to avoid misleading conclusions or erroneous interpretations. Facing the lack of comprehensive studies on urine metabolome stability, the present work performed a follow-up of potential modiﬁcations in urinary chemical proﬁle using {LC}-{HRMS} on the basis of two parameters: the storage temperature (þ4 C, À20 C, À80 C and freeze-dried stored at À80 C) and the storage duration (5e144 days). Both {HILIC} and {RP} chromatographies have been implemented in order to globally monitor the urinary metabolome. Using an original data processing associated to univariate and multivariate data analysis, our study conﬁrms that chemical proﬁles of urine samples stored at þ4 C are very rapidly modiﬁed, as observed for instance for compounds such as:Nacetyl Glycine, Adenosine, 4-Amino benzoic acid, N-Amino diglycine, creatine, glucuronic acid, 3hydroxy-benzoic acid, pyridoxal, L-pyroglutamic acid, shikimic acid, succinic acid, thymidine, trigonelline and valeryl-carnitine, while it also demonstrates that urine samples stored at À20 C exhibit a global stability over a long period with no major modiﬁcations compared to À80 C condition. This study is the ﬁrst to investigate long term stability of urine samples and report potential modiﬁcations in the urinary metabolome, using both targeted approach monitoring individually a large number (n {\textgreater} 200) of urinary metabolites and an untargeted strategy enabling assessing for global impact of storage conditions.},
	pages = {99--107},
	journaltitle = {Analytica Chimica Acta},
	author = {Laparre, Jérôme and Kaabia, Zied and Mooney, Mark and Buckley, Tom and Sherry, Mark and Le Bizec, Bruno and Dervilly-Pinel, Gaud},
	urldate = {2019-03-29},
	date = {2017-01},
	langid = {english},
	file = {Laparre et al. - 2017 - Impact of storage conditions on the urinary metabo.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\FC6RG2QV\\Laparre et al. - 2017 - Impact of storage conditions on the urinary metabo.pdf:application/pdf}
}

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Facing the lack of comprehensive studies on urine metabolome stability, the present work performed a follow-up of potential modiﬁcations in urinary chemical proﬁle using LC-HRMS on the basis of two parameters: the storage temperature (þ4 \u0014C, À20 \u0014C, À80 \u0014C and freeze-dried stored at À80 \u0014C) and the storage duration (5e144 days). Both HILIC and RP chromatographies have been implemented in order to globally monitor the urinary metabolome. Using an original data processing associated to univariate and multivariate data analysis, our study conﬁrms that chemical proﬁles of urine samples stored at þ4 \u0014C are very rapidly modiﬁed, as observed for instance for compounds such as:Nacetyl Glycine, Adenosine, 4-Amino benzoic acid, N-Amino diglycine, creatine, glucuronic acid, 3hydroxy-benzoic acid, pyridoxal, L-pyroglutamic acid, shikimic acid, succinic acid, thymidine, trigonelline and valeryl-carnitine, while it also demonstrates that urine samples stored at À20 \u0014C exhibit a global stability over a long period with no major modiﬁcations compared to À80 \u0014C condition. 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