Impact of storage conditions on the urinary metabolomics fingerprint. Laparre, J., Kaabia, Z., Mooney, M., Buckley, T., Sherry, M., Le Bizec, B., & Dervilly-Pinel, G. 951:99–107.
Impact of storage conditions on the urinary metabolomics fingerprint [link]Paper  doi  abstract   bibtex   
Urine stability during storage is essential in metabolomics to avoid misleading conclusions or erroneous interpretations. Facing the lack of comprehensive studies on urine metabolome stability, the present work performed a follow-up of potential modifications in urinary chemical profile using LC-HRMS on the basis of two parameters: the storage temperature (þ4 C, À20 C, À80 C and freeze-dried stored at À80 C) and the storage duration (5e144 days). Both HILIC and RP chromatographies have been implemented in order to globally monitor the urinary metabolome. Using an original data processing associated to univariate and multivariate data analysis, our study confirms that chemical profiles of urine samples stored at þ4 C are very rapidly modified, as observed for instance for compounds such as:Nacetyl Glycine, Adenosine, 4-Amino benzoic acid, N-Amino diglycine, creatine, glucuronic acid, 3hydroxy-benzoic acid, pyridoxal, L-pyroglutamic acid, shikimic acid, succinic acid, thymidine, trigonelline and valeryl-carnitine, while it also demonstrates that urine samples stored at À20 C exhibit a global stability over a long period with no major modifications compared to À80 C condition. This study is the first to investigate long term stability of urine samples and report potential modifications in the urinary metabolome, using both targeted approach monitoring individually a large number (n \textgreater 200) of urinary metabolites and an untargeted strategy enabling assessing for global impact of storage conditions.
@article{laparre_impact_2017,
	title = {Impact of storage conditions on the urinary metabolomics fingerprint},
	volume = {951},
	issn = {00032670},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0003267016314052},
	doi = {10.1016/j.aca.2016.11.055},
	abstract = {Urine stability during storage is essential in metabolomics to avoid misleading conclusions or erroneous interpretations. Facing the lack of comprehensive studies on urine metabolome stability, the present work performed a follow-up of potential modifications in urinary chemical profile using {LC}-{HRMS} on the basis of two parameters: the storage temperature (þ4 C, À20 C, À80 C and freeze-dried stored at À80 C) and the storage duration (5e144 days). Both {HILIC} and {RP} chromatographies have been implemented in order to globally monitor the urinary metabolome. Using an original data processing associated to univariate and multivariate data analysis, our study confirms that chemical profiles of urine samples stored at þ4 C are very rapidly modified, as observed for instance for compounds such as:Nacetyl Glycine, Adenosine, 4-Amino benzoic acid, N-Amino diglycine, creatine, glucuronic acid, 3hydroxy-benzoic acid, pyridoxal, L-pyroglutamic acid, shikimic acid, succinic acid, thymidine, trigonelline and valeryl-carnitine, while it also demonstrates that urine samples stored at À20 C exhibit a global stability over a long period with no major modifications compared to À80 C condition. This study is the first to investigate long term stability of urine samples and report potential modifications in the urinary metabolome, using both targeted approach monitoring individually a large number (n {\textgreater} 200) of urinary metabolites and an untargeted strategy enabling assessing for global impact of storage conditions.},
	pages = {99--107},
	journaltitle = {Analytica Chimica Acta},
	author = {Laparre, Jérôme and Kaabia, Zied and Mooney, Mark and Buckley, Tom and Sherry, Mark and Le Bizec, Bruno and Dervilly-Pinel, Gaud},
	urldate = {2019-03-29},
	date = {2017-01},
	langid = {english},
	file = {Laparre et al. - 2017 - Impact of storage conditions on the urinary metabo.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\FC6RG2QV\\Laparre et al. - 2017 - Impact of storage conditions on the urinary metabo.pdf:application/pdf}
}

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