An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water

An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water. Lapizco-Encinas, B. H., Davalos, R. V., Simmons, B. A., Cummings, E. B., & Fintschenko, Y. J Microbiol Methods, 62(3):317-26, 2005. Lapizco-Encinas, Blanca H Davalos, Rafael V Simmons, Blake A Cummings, Eric B Fintschenko, Yolanda Journal Article Research Support, Non-U.S. Gov't Netherlands 2005/06/09 J Microbiol Methods. 2005 Sep;62(3):317-26. doi: 10.1016/j.mimet.2005.04.027.
doi abstract bibtex

Dielectrophoresis (DEP), the motion of a particle caused by an applied electric field gradient, can concentrate microorganisms non-destructively. In insulator-based dielectrophoresis (iDEP) insulating microstructures produce non-uniform electric fields to drive DEP in microsystems. This article describes the performance of an iDEP device in removing and concentrating bacterial cells, spores and viruses while operated with a DC applied electric field and pressure gradient. Such a device can selectively trap particles when dielectrophoresis overcomes electrokinesis or advection. The dielectrophoretic trapping behavior of labeled microorganisms in a glass-etched iDEP device was observed over a wide range of DC applied electric fields. When fields higher than a particle-specific threshold are applied, particles are reversibly trapped in the device. Experiments with Bacillus subtilis spores and the Tobacco Mosaic Virus (TMV) exhibited higher trapping thresholds than those of bacterial cells. The iDEP device was characterized in terms of concentration factor and removal efficiency. Under the experimental conditions used in this study with an initial dilution of 1 x 105 cells/ml, concentration factors of the order of 3000x and removal efficiencies approaching 100% were observed with Escherichia coli cells. These results are the first characterization of an iDEP device for the concentration and removal of microbes in water.

@article{RN246,
   author = {Lapizco-Encinas, B. H. and Davalos, R. V. and Simmons, B. A. and Cummings, E. B. and Fintschenko, Y.},
   title = {An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water},
   journal = {J Microbiol Methods},
   volume = {62},
   number = {3},
   pages = {317-26},
   note = {Lapizco-Encinas, Blanca H
Davalos, Rafael V
Simmons, Blake A
Cummings, Eric B
Fintschenko, Yolanda
Journal Article
Research Support, Non-U.S. Gov't
Netherlands
2005/06/09
J Microbiol Methods. 2005 Sep;62(3):317-26. doi: 10.1016/j.mimet.2005.04.027.},
   abstract = {Dielectrophoresis (DEP), the motion of a particle caused by an applied electric field gradient, can concentrate microorganisms non-destructively. In insulator-based dielectrophoresis (iDEP) insulating microstructures produce non-uniform electric fields to drive DEP in microsystems. This article describes the performance of an iDEP device in removing and concentrating bacterial cells, spores and viruses while operated with a DC applied electric field and pressure gradient. Such a device can selectively trap particles when dielectrophoresis overcomes electrokinesis or advection. The dielectrophoretic trapping behavior of labeled microorganisms in a glass-etched iDEP device was observed over a wide range of DC applied electric fields. When fields higher than a particle-specific threshold are applied, particles are reversibly trapped in the device. Experiments with Bacillus subtilis spores and the Tobacco Mosaic Virus (TMV) exhibited higher trapping thresholds than those of bacterial cells. The iDEP device was characterized in terms of concentration factor and removal efficiency. Under the experimental conditions used in this study with an initial dilution of 1 x 105 cells/ml, concentration factors of the order of 3000x and removal efficiencies approaching 100% were observed with Escherichia coli cells. These results are the first characterization of an iDEP device for the concentration and removal of microbes in water.},
   keywords = {Bacillus subtilis/isolation & purification
Electrophoresis/*instrumentation
Equipment Design
Escherichia coli/isolation & purification
Microbiological Techniques/*instrumentation
Microfluidic Analytical Techniques
Spores, Bacterial/isolation & purification
Tobacco Mosaic Virus/isolation & purification
*Water Microbiology},
   ISSN = {0167-7012 (Print)
0167-7012},
   DOI = {10.1016/j.mimet.2005.04.027},
   year = {2005},
   type = {Journal Article}
}

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