Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast. Lartigue, C., Vashee, S., Algire, M., A., Chuang, R., Benders, G., A., Ma, L., Noskov, V., N., Denisova, E., A., Gibson, D., G., Assad-Garcia, N., Alperovich, N., Thomas, D., W., Merryman, C., Hutchison III, C., A., Smith, H., O., Venter, J., C., & Glass, J., I. Science, 2009. abstract bibtex We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here, we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid, and then transplanted it into Mycoplama capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered using yeast genetic systems, and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.
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title = {Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast},
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abstract = {We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here, we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid, and then transplanted it into Mycoplama capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered using yeast genetic systems, and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.},
bibtype = {article},
author = {Lartigue, Carole and Vashee, Sanjay and Algire, Mikkel A and Chuang, Ray-Yuan and Benders, Gwynedd A and Ma, Li and Noskov, Vladimir N and Denisova, Evgeniya A and Gibson, Daniel G and Assad-Garcia, Nacyra and Alperovich, Nina and Thomas, David W and Merryman, Chuck and Hutchison III, Clyde A and Smith, Hamilton O and Venter, J Craig and Glass, John I},
journal = {Science}
}
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