@ARTICLE{Lebedev1998,
author={Lebedev, D.V. and Shearer, S. and Knauf, P.A.},
title={Volume-sensitive amino acid transport during Regulatory Volume Decrease (RVD) in HL-60 cells},
journal={FASEB Journal},
year={1998},
volume={12},
number={5},
pages={A1023},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749332327&partnerID=40&md5=ebb384cc7bd32ca75a267c2bf7cb0f78},
affiliation={Univ. of Rochester Med. Ctr., Rochester, NY 14642, United States},
abstract={RVD in promyelocytic leukemic HL-60 cells is accomplished primarily by K+ and Cl- loss triggered by opening of volume-sensitive Cl channels. To evaluate the involvement of other transport processes in RVD. we have measured RVD in medium with the [K]o[Cl]o product set to approximate [K]i[Cl]i. Triple-label radioisotope measurements of cell water and Cl content indicate that under these conditions cells swollen in 50% tonicity medium still show approximately 40% of the normal volume recovery, even though cell Cl content remains unchanged. Volume recovery is inhibited by 50μM DIDS, which inhibits volume-sensitive Cl efflux during normal RVD. There was a significant (over 5 fold) increase in the rate of release of ninhydrin-positive substances (very likely amino acids) under hypotonic conditions. Both amino acid release and unidirectional 36Cl efflux were inhibited by an oxonol dye WW781 (IC50 = 4.5±1.3μM and 2.1±0.3μM, respectively); the differential effects of WW781 on Cl- and amino acid efflux fit a model with more than one pathway for amino acid transport. This is supported by evidence that external ATP (10 mM) has different effects on 36Cl efflux and on 3H-D-aspartate efflux in hypotonic medium, implying that part of the volume-sensitive amino acid efflux goes via a different pathway than chloride.},
correspondence_address1={Lebedev, D.V.; Univ. of Rochester Med. Ctr., Rochester, NY 14642, United States},
issn={08926638},
coden={FAJOE},
language={English},
abbrev_source_title={FASEB J.},
document_type={Article},
source={Scopus},
}

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Ctr., Rochester, NY 14642, United States","abstract":"RVD in promyelocytic leukemic HL-60 cells is accomplished primarily by K+ and Cl- loss triggered by opening of volume-sensitive Cl channels. To evaluate the involvement of other transport processes in RVD. we have measured RVD in medium with the [K]o[Cl]o product set to approximate [K]i[Cl]i. Triple-label radioisotope measurements of cell water and Cl content indicate that under these conditions cells swollen in 50% tonicity medium still show approximately 40% of the normal volume recovery, even though cell Cl content remains unchanged. Volume recovery is inhibited by 50μM DIDS, which inhibits volume-sensitive Cl efflux during normal RVD. There was a significant (over 5 fold) increase in the rate of release of ninhydrin-positive substances (very likely amino acids) under hypotonic conditions. Both amino acid release and unidirectional 36Cl efflux were inhibited by an oxonol dye WW781 (IC50 = 4.5±1.3μM and 2.1±0.3μM, respectively); the differential effects of WW781 on Cl- and amino acid efflux fit a model with more than one pathway for amino acid transport. This is supported by evidence that external ATP (10 mM) has different effects on 36Cl efflux and on 3H-D-aspartate efflux in hypotonic medium, implying that part of the volume-sensitive amino acid efflux goes via a different pathway than chloride.","correspondence_address1":"Lebedev, D.V.; Univ. of Rochester Med. 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Triple-label radioisotope measurements of cell water and Cl content indicate that under these conditions cells swollen in 50% tonicity medium still show approximately 40% of the normal volume recovery, even though cell Cl content remains unchanged. Volume recovery is inhibited by 50μM DIDS, which inhibits volume-sensitive Cl efflux during normal RVD. There was a significant (over 5 fold) increase in the rate of release of ninhydrin-positive substances (very likely amino acids) under hypotonic conditions. Both amino acid release and unidirectional 36Cl efflux were inhibited by an oxonol dye WW781 (IC50 = 4.5±1.3μM and 2.1±0.3μM, respectively); the differential effects of WW781 on Cl- and amino acid efflux fit a model with more than one pathway for amino acid transport. This is supported by evidence that external ATP (10 mM) has different effects on 36Cl efflux and on 3H-D-aspartate efflux in hypotonic medium, implying that part of the volume-sensitive amino acid efflux goes via a different pathway than chloride.},\r\ncorrespondence_address1={Lebedev, D.V.; Univ. of Rochester Med. 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