Microfluidic electroporation of robust 10-microm vesicles for manipulation of picoliter volumes. Lee, E. S., Robinson, D., Rognlien, J. L., Harnett, C. K., Simmons, B. A., Bowe Ellis, C. R., & Davalos, R. V. Bioelectrochemistry, 69(1):117-25, 2006. Lee, Eunice S Robinson, David Rognlien, Judith L Harnett, Cindy K Simmons, Blake A Bowe Ellis, C R Davalos, Rafael V Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Netherlands 2006/02/18 Bioelectrochemistry. 2006 Sep;69(1):117-25. doi: 10.1016/j.bioelechem.2005.12.002. Epub 2006 Feb 17.doi abstract bibtex We present a new way to transport and handle picoliter volumes of analytes in a microfluidic context through electrically monitored electroporation of 10-25 microm vesicles. In this method, giant vesicles are used to isolate analytes in a microfluidic environment. Once encapsulated inside a vesicle, contents will not diffuse and become diluted when exposed to pressure-driven flow. Two vesicle compositions have been developed that are robust enough to withstand electrical and mechanical manipulation in a microfluidic context. These vesicles can be guided and trapped, with controllable transfer of material into or out of their confined environment. Through electroporation, vesicles can serve as containers that can be opened when mixing and diffusion are desired, and closed during transport and analysis. Both vesicle compositions contain lecithin, an ethoxylated phospholipid, and a polyelectrolyte. Their performance is compared using a prototype microfluidic device and a simple circuit model. It was observed that the energy density threshold required to induce breakdown was statistically equivalent between compositions, 10.2+/-5.0 mJ/m2 for the first composition and 10.5+/-1.8 mJ/m2 for the second. This work demonstrates the feasibility of using giant, robust vesicles with microfluidic electroporation technology to manipulate picoliter volumes on-chip.
@article{RN245,
author = {Lee, E. S. and Robinson, D. and Rognlien, J. L. and Harnett, C. K. and Simmons, B. A. and Bowe Ellis, C. R. and Davalos, R. V.},
title = {Microfluidic electroporation of robust 10-microm vesicles for manipulation of picoliter volumes},
journal = {Bioelectrochemistry},
volume = {69},
number = {1},
pages = {117-25},
note = {Lee, Eunice S
Robinson, David
Rognlien, Judith L
Harnett, Cindy K
Simmons, Blake A
Bowe Ellis, C R
Davalos, Rafael V
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Netherlands
2006/02/18
Bioelectrochemistry. 2006 Sep;69(1):117-25. doi: 10.1016/j.bioelechem.2005.12.002. Epub 2006 Feb 17.},
abstract = {We present a new way to transport and handle picoliter volumes of analytes in a microfluidic context through electrically monitored electroporation of 10-25 microm vesicles. In this method, giant vesicles are used to isolate analytes in a microfluidic environment. Once encapsulated inside a vesicle, contents will not diffuse and become diluted when exposed to pressure-driven flow. Two vesicle compositions have been developed that are robust enough to withstand electrical and mechanical manipulation in a microfluidic context. These vesicles can be guided and trapped, with controllable transfer of material into or out of their confined environment. Through electroporation, vesicles can serve as containers that can be opened when mixing and diffusion are desired, and closed during transport and analysis. Both vesicle compositions contain lecithin, an ethoxylated phospholipid, and a polyelectrolyte. Their performance is compared using a prototype microfluidic device and a simple circuit model. It was observed that the energy density threshold required to induce breakdown was statistically equivalent between compositions, 10.2+/-5.0 mJ/m2 for the first composition and 10.5+/-1.8 mJ/m2 for the second. This work demonstrates the feasibility of using giant, robust vesicles with microfluidic electroporation technology to manipulate picoliter volumes on-chip.},
keywords = {Electroporation/*instrumentation/*methods
Lipids/chemistry
Microfluidics/*instrumentation/*methods
Models, Theoretical
Particle Size
Time Factors},
ISSN = {1567-5394 (Print)
1567-5394},
DOI = {10.1016/j.bioelechem.2005.12.002},
year = {2006},
type = {Journal Article}
}
Downloads: 0
{"_id":"Bkv2tEqPR4rgZxDFp","bibbaseid":"lee-robinson-rognlien-harnett-simmons-boweellis-davalos-microfluidicelectroporationofrobust10micromvesiclesformanipulationofpicolitervolumes-2006","author_short":["Lee, E. S.","Robinson, D.","Rognlien, J. L.","Harnett, C. K.","Simmons, B. A.","Bowe Ellis, C. R.","Davalos, R. V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Lee"],"firstnames":["E.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Robinson"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Rognlien"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Harnett"],"firstnames":["C.","K."],"suffixes":[]},{"propositions":[],"lastnames":["Simmons"],"firstnames":["B.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Bowe","Ellis"],"firstnames":["C.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Microfluidic electroporation of robust 10-microm vesicles for manipulation of picoliter volumes","journal":"Bioelectrochemistry","volume":"69","number":"1","pages":"117-25","note":"Lee, Eunice S Robinson, David Rognlien, Judith L Harnett, Cindy K Simmons, Blake A Bowe Ellis, C R Davalos, Rafael V Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Netherlands 2006/02/18 Bioelectrochemistry. 2006 Sep;69(1):117-25. doi: 10.1016/j.bioelechem.2005.12.002. Epub 2006 Feb 17.","abstract":"We present a new way to transport and handle picoliter volumes of analytes in a microfluidic context through electrically monitored electroporation of 10-25 microm vesicles. In this method, giant vesicles are used to isolate analytes in a microfluidic environment. Once encapsulated inside a vesicle, contents will not diffuse and become diluted when exposed to pressure-driven flow. Two vesicle compositions have been developed that are robust enough to withstand electrical and mechanical manipulation in a microfluidic context. These vesicles can be guided and trapped, with controllable transfer of material into or out of their confined environment. Through electroporation, vesicles can serve as containers that can be opened when mixing and diffusion are desired, and closed during transport and analysis. Both vesicle compositions contain lecithin, an ethoxylated phospholipid, and a polyelectrolyte. Their performance is compared using a prototype microfluidic device and a simple circuit model. It was observed that the energy density threshold required to induce breakdown was statistically equivalent between compositions, 10.2+/-5.0 mJ/m2 for the first composition and 10.5+/-1.8 mJ/m2 for the second. This work demonstrates the feasibility of using giant, robust vesicles with microfluidic electroporation technology to manipulate picoliter volumes on-chip.","keywords":"Electroporation/*instrumentation/*methods Lipids/chemistry Microfluidics/*instrumentation/*methods Models, Theoretical Particle Size Time Factors","issn":"1567-5394 (Print) 1567-5394","doi":"10.1016/j.bioelechem.2005.12.002","year":"2006","bibtex":"@article{RN245,\n author = {Lee, E. S. and Robinson, D. and Rognlien, J. L. and Harnett, C. K. and Simmons, B. A. and Bowe Ellis, C. R. and Davalos, R. V.},\n title = {Microfluidic electroporation of robust 10-microm vesicles for manipulation of picoliter volumes},\n journal = {Bioelectrochemistry},\n volume = {69},\n number = {1},\n pages = {117-25},\n note = {Lee, Eunice S\nRobinson, David\nRognlien, Judith L\nHarnett, Cindy K\nSimmons, Blake A\nBowe Ellis, C R\nDavalos, Rafael V\nComparative Study\nJournal Article\nResearch Support, Non-U.S. Gov't\nResearch Support, U.S. Gov't, Non-P.H.S.\nNetherlands\n2006/02/18\nBioelectrochemistry. 2006 Sep;69(1):117-25. doi: 10.1016/j.bioelechem.2005.12.002. Epub 2006 Feb 17.},\n abstract = {We present a new way to transport and handle picoliter volumes of analytes in a microfluidic context through electrically monitored electroporation of 10-25 microm vesicles. In this method, giant vesicles are used to isolate analytes in a microfluidic environment. Once encapsulated inside a vesicle, contents will not diffuse and become diluted when exposed to pressure-driven flow. Two vesicle compositions have been developed that are robust enough to withstand electrical and mechanical manipulation in a microfluidic context. These vesicles can be guided and trapped, with controllable transfer of material into or out of their confined environment. Through electroporation, vesicles can serve as containers that can be opened when mixing and diffusion are desired, and closed during transport and analysis. Both vesicle compositions contain lecithin, an ethoxylated phospholipid, and a polyelectrolyte. Their performance is compared using a prototype microfluidic device and a simple circuit model. It was observed that the energy density threshold required to induce breakdown was statistically equivalent between compositions, 10.2+/-5.0 mJ/m2 for the first composition and 10.5+/-1.8 mJ/m2 for the second. This work demonstrates the feasibility of using giant, robust vesicles with microfluidic electroporation technology to manipulate picoliter volumes on-chip.},\n keywords = {Electroporation/*instrumentation/*methods\nLipids/chemistry\nMicrofluidics/*instrumentation/*methods\nModels, Theoretical\nParticle Size\nTime Factors},\n ISSN = {1567-5394 (Print)\n1567-5394},\n DOI = {10.1016/j.bioelechem.2005.12.002},\n year = {2006},\n type = {Journal Article}\n}\n\n","author_short":["Lee, E. S.","Robinson, D.","Rognlien, J. L.","Harnett, C. K.","Simmons, B. A.","Bowe Ellis, C. R.","Davalos, R. V."],"key":"RN245","id":"RN245","bibbaseid":"lee-robinson-rognlien-harnett-simmons-boweellis-davalos-microfluidicelectroporationofrobust10micromvesiclesformanipulationofpicolitervolumes-2006","role":"author","urls":{},"keyword":["Electroporation/*instrumentation/*methods Lipids/chemistry Microfluidics/*instrumentation/*methods Models","Theoretical Particle Size Time Factors"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["electroporation/*instrumentation/*methods lipids/chemistry microfluidics/*instrumentation/*methods models","theoretical particle size time factors"],"search_terms":["microfluidic","electroporation","robust","microm","vesicles","manipulation","picoliter","volumes","lee","robinson","rognlien","harnett","simmons","bowe ellis","davalos"],"title":"Microfluidic electroporation of robust 10-microm vesicles for manipulation of picoliter volumes","year":2006}