Dynamics of Notch-Dependent Transcriptional Bursting in its Native Context. Lee, C., Shin, H., & Kimble, J. bioRxiv, December, 2018. Publisher: Cold Spring Harbor Laboratory Section: New Results![Dynamics of Notch-Dependent Transcriptional Bursting in its Native Context [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex \textlessh3\textgreaterSUMMARY\textless/h3\textgreater \textlessp\textgreaterTranscription is well known to be inherently stochastic and episodic, but the regulation of transcriptional dynamics is not well understood. Here we analyze how Notch signaling modulates transcriptional bursting during animal development. Our focus is Notch regulation of transcription in germline stem cells of the nematode C. elegans. Using the MS2 system to visualize nascent transcripts and live imaging to record dynamics, we analyze bursting as a function of position within the intact animal. We find that Notch-dependent transcriptional activation is indeed “bursty”; that wild-type Notch modulates burst duration (ON-time) rather than duration of pauses between bursts (OFF-time) or mean burst intensity; and that a mutant Notch receptor, which is compromised for assembly into the Notch transcription factor complex, primarily modifies burst size (duration x intensity). To our knowledge, this work is the first to visualize regulation of metazoan transcriptional bursting by a canonical signaling pathway in its native context.\textless/p\textgreater\textlessh3\textgreaterHIGHLIGHTS\textless/h3\textgreater \textlessp\textgreaterNotch-dependent transcriptional bursts are spatially graded across the stem cell pool\textless/p\textgreater\textlessp\textgreaterBurst duration is the key determinant of Notch-dependent transcriptional probability\textless/p\textgreater\textlessp\textgreaterNotch NICD strength influences both burst duration and intensity\textless/p\textgreater\textlessp\textgreaterNotch dynamics are largely stochastic for consecutive bursts at the same chromosomal locus\textless/p\textgreater
@article{lee_dynamics_2018,
title = {Dynamics of {Notch}-{Dependent} {Transcriptional} {Bursting} in its {Native} {Context}},
copyright = {© 2018, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/},
url = {https://www.biorxiv.org/content/10.1101/496638v1},
doi = {10.1101/496638},
abstract = {{\textless}h3{\textgreater}SUMMARY{\textless}/h3{\textgreater} {\textless}p{\textgreater}Transcription is well known to be inherently stochastic and episodic, but the regulation of transcriptional dynamics is not well understood. Here we analyze how Notch signaling modulates transcriptional bursting during animal development. Our focus is Notch regulation of transcription in germline stem cells of the nematode \textit{C. elegans}. Using the MS2 system to visualize nascent transcripts and live imaging to record dynamics, we analyze bursting as a function of position within the intact animal. We find that Notch-dependent transcriptional activation is indeed “bursty”; that wild-type Notch modulates burst duration (ON-time) rather than duration of pauses between bursts (OFF-time) or mean burst intensity; and that a mutant Notch receptor, which is compromised for assembly into the Notch transcription factor complex, primarily modifies burst size (duration x intensity). To our knowledge, this work is the first to visualize regulation of metazoan transcriptional bursting by a canonical signaling pathway in its native context.{\textless}/p{\textgreater}{\textless}h3{\textgreater}HIGHLIGHTS{\textless}/h3{\textgreater} {\textless}p{\textgreater}Notch-dependent transcriptional bursts are spatially graded across the stem cell pool{\textless}/p{\textgreater}{\textless}p{\textgreater}Burst duration is the key determinant of Notch-dependent transcriptional probability{\textless}/p{\textgreater}{\textless}p{\textgreater}Notch NICD strength influences both burst duration and intensity{\textless}/p{\textgreater}{\textless}p{\textgreater}Notch dynamics are largely stochastic for consecutive bursts at the same chromosomal locus{\textless}/p{\textgreater}},
language = {en},
urldate = {2020-08-27},
journal = {bioRxiv},
author = {Lee, ChangHwan and Shin, Heaji and Kimble, Judith},
month = dec,
year = {2018},
note = {Publisher: Cold Spring Harbor Laboratory
Section: New Results},
pages = {496638},
}
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