@article{leitz_laser-based_2003,
	title = {Laser-based micromanipulation for separation and identification of individual {Frankia} vesicles},
	volume = {224},
	issn = {0378-1097},
	doi = {10/bf78w8},
	abstract = {In studies of symbiotic efficiency it is of great importance to identify and separate individual Frankia strains from a nodule. Therefore, a new laser-based micromanipulation technique has been developed in which individual vesicles from root nodules of two Frankia-Alnus symbioses have been successfully cut loose and separated from clusters of vesicles in sterile conditions under light microscopy using a laser scalpel and optical tweezers. Vesicles from the Alnus incana-Frankia AvCIl symbiosis were successfully isolated and grown in culture using this technique. The DNA from both Frankia sources was amplified by polymerase chain reaction (PCR). The work shows that a combination of laser-based manipulation techniques and PCR can be used for the separation and study of individual vesicles. This novel laser-based micromanipulation technique opens up various new possibilities, for instance, to study whether several Frankia strains can grow simultaneously in the same root nodule. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.},
	language = {English},
	number = {1},
	journal = {Fems Microbiology Letters},
	author = {Leitz, G. and Lundberg, C. and Fallman, E. and Axner, O. and Sellstedt, A.},
	month = jul,
	year = {2003},
	note = {Place: Amsterdam
Publisher: Elsevier Science Bv
WOS:000184248400014},
	keywords = {Frankia, bacteria, casuarina, dna, hydrogen metabolism, laser scalpel, manipulation, nitrogen-fixation, optical tweezers, pcr, polymerase chain-reaction, rflp, root-nodules, strains},
	pages = {97--100},
}

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Vesicles from the Alnus incana-Frankia AvCIl symbiosis were successfully isolated and grown in culture using this technique. The DNA from both Frankia sources was amplified by polymerase chain reaction (PCR). The work shows that a combination of laser-based manipulation techniques and PCR can be used for the separation and study of individual vesicles. This novel laser-based micromanipulation technique opens up various new possibilities, for instance, to study whether several Frankia strains can grow simultaneously in the same root nodule. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.","language":"English","number":"1","journal":"Fems Microbiology Letters","author":[{"propositions":[],"lastnames":["Leitz"],"firstnames":["G."],"suffixes":[]},{"propositions":[],"lastnames":["Lundberg"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Fallman"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Axner"],"firstnames":["O."],"suffixes":[]},{"propositions":[],"lastnames":["Sellstedt"],"firstnames":["A."],"suffixes":[]}],"month":"July","year":"2003","note":"Place: Amsterdam Publisher: Elsevier Science Bv WOS:000184248400014","keywords":"Frankia, bacteria, casuarina, dna, hydrogen metabolism, laser scalpel, manipulation, nitrogen-fixation, optical tweezers, pcr, polymerase chain-reaction, rflp, root-nodules, strains","pages":"97–100","bibtex":"@article{leitz_laser-based_2003,\n\ttitle = {Laser-based micromanipulation for separation and identification of individual {Frankia} vesicles},\n\tvolume = {224},\n\tissn = {0378-1097},\n\tdoi = {10/bf78w8},\n\tabstract = {In studies of symbiotic efficiency it is of great importance to identify and separate individual Frankia strains from a nodule. Therefore, a new laser-based micromanipulation technique has been developed in which individual vesicles from root nodules of two Frankia-Alnus symbioses have been successfully cut loose and separated from clusters of vesicles in sterile conditions under light microscopy using a laser scalpel and optical tweezers. Vesicles from the Alnus incana-Frankia AvCIl symbiosis were successfully isolated and grown in culture using this technique. The DNA from both Frankia sources was amplified by polymerase chain reaction (PCR). The work shows that a combination of laser-based manipulation techniques and PCR can be used for the separation and study of individual vesicles. This novel laser-based micromanipulation technique opens up various new possibilities, for instance, to study whether several Frankia strains can grow simultaneously in the same root nodule. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.},\n\tlanguage = {English},\n\tnumber = {1},\n\tjournal = {Fems Microbiology Letters},\n\tauthor = {Leitz, G. and Lundberg, C. and Fallman, E. and Axner, O. and Sellstedt, A.},\n\tmonth = jul,\n\tyear = {2003},\n\tnote = {Place: Amsterdam\nPublisher: Elsevier Science Bv\nWOS:000184248400014},\n\tkeywords = {Frankia, bacteria, casuarina, dna, hydrogen metabolism, laser scalpel, manipulation, nitrogen-fixation, optical tweezers, pcr, polymerase chain-reaction, rflp, root-nodules, strains},\n\tpages = {97--100},\n}\n\n\n\n","author_short":["Leitz, G.","Lundberg, C.","Fallman, E.","Axner, O.","Sellstedt, A."],"key":"leitz_laser-based_2003","id":"leitz_laser-based_2003","bibbaseid":"leitz-lundberg-fallman-axner-sellstedt-laserbasedmicromanipulationforseparationandidentificationofindividualfrankiavesicles-2003","role":"author","urls":{},"keyword":["Frankia","bacteria","casuarina","dna","hydrogen metabolism","laser scalpel","manipulation","nitrogen-fixation","optical tweezers","pcr","polymerase chain-reaction","rflp","root-nodules","strains"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs","fvfkWcShg3Mybjoog","Tu3jPdZyJF3j547xT","3zTPPmKj8BiTcpc6C"],"keywords":["frankia","bacteria","casuarina","dna","hydrogen metabolism","laser scalpel","manipulation","nitrogen-fixation","optical tweezers","pcr","polymerase chain-reaction","rflp","root-nodules","strains"],"search_terms":["laser","based","micromanipulation","separation","identification","individual","frankia","vesicles","leitz","lundberg","fallman","axner","sellstedt"],"title":"Laser-based micromanipulation for separation and identification of individual Frankia vesicles","year":2003}