Efficient and chemoselective surface immobilization of proteins by using aniline-catalyzed oxime chemistry. Lempens, E. H M, Helms, B. A, Merkx, M., & Meijer, E W Chembiochem, 10(4):658–662, Germany, March, 2009. abstract bibtex Site-specific immobilization of peptides and proteins is crucial to ensure their functionality in surface-based assays. We report the use of aniline-catalyzed oxime ligations as a very efficient and broadly applicable method to covalently attach the N terminus of proteins and peptides to a surface functionalized with alkoxy-amine groups.
@ARTICLE{Lempens2009-rg,
title = "Efficient and chemoselective surface immobilization of proteins
by using aniline-catalyzed oxime chemistry",
author = "Lempens, Edith H M and Helms, Brett A and Merkx, Maarten and
Meijer, E W",
abstract = "Site-specific immobilization of peptides and proteins is crucial
to ensure their functionality in surface-based assays. We report
the use of aniline-catalyzed oxime ligations as a very efficient
and broadly applicable method to covalently attach the N terminus
of proteins and peptides to a surface functionalized with
alkoxy-amine groups.",
journal = "Chembiochem",
volume = 10,
number = 4,
pages = "658--662",
month = mar,
year = 2009,
address = "Germany",
language = "en"
}
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