Efficient and chemoselective surface immobilization of proteins by using aniline-catalyzed oxime chemistry. Lempens, E. H M, Helms, B. A, Merkx, M., & Meijer, E W Chembiochem, 10(4):658–662, Germany, March, 2009.
abstract   bibtex   
Site-specific immobilization of peptides and proteins is crucial to ensure their functionality in surface-based assays. We report the use of aniline-catalyzed oxime ligations as a very efficient and broadly applicable method to covalently attach the N terminus of proteins and peptides to a surface functionalized with alkoxy-amine groups.
@ARTICLE{Lempens2009-rg,
  title    = "Efficient and chemoselective surface immobilization of proteins
              by using aniline-catalyzed oxime chemistry",
  author   = "Lempens, Edith H M and Helms, Brett A and Merkx, Maarten and
              Meijer, E W",
  abstract = "Site-specific immobilization of peptides and proteins is crucial
              to ensure their functionality in surface-based assays. We report
              the use of aniline-catalyzed oxime ligations as a very efficient
              and broadly applicable method to covalently attach the N terminus
              of proteins and peptides to a surface functionalized with
              alkoxy-amine groups.",
  journal  = "Chembiochem",
  volume   =  10,
  number   =  4,
  pages    = "658--662",
  month    =  mar,
  year     =  2009,
  address  = "Germany",
  language = "en"
}

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