@article{lertwimol_facile_2023,
	title = {A facile strategy for promoting cell adhesion and function on three-dimensional printed hydrogels using photocurable epsilon-poly-{L}-lysine},
	volume = {196},
	issn = {0014-3057},
	doi = {10.1016/j.eurpolymj.2023.112245},
	abstract = {This study introduces a new persuasive strategy for promoting cell adhesion and function on three-dimensional (3D) printed hydrogels by incorporating photocurable epsilon-poly-L-lysine (EPL) as the cell adhesive molecule. Two different polymers, dextran (Dex) and EPL, were first modified using methacrylic anhydride and glycidyl methacrylate, respectively, to obtain the methacrylate-functionalized polymers: Dex-MA and EPL-MA. Subsequently, these modified polymers were formulated with poly(ethylene glycol dimethacrylate) (PEGDMA) before fabricating the 3D-printed hydrogels through digital light processing (DLP). The effects of the PEGDMA, Dex-MA, and EPL-MA ratios in the formulation on the physical properties of 3D-printed hydrogel and their biological responses were assessed. The 3D-printed specimens possessed compressive moduli in the range of 280-520 kPa. SEM/EDS analysis confirmed the presence of nitrogen in the hydrogels. The ninhydrin assay revealed that the EPL-MA content fell in the range of 0-0.15 mg/mg. All prepared hydrogels exhibited non-cytotoxic behavior toward NIH/3T3 fibroblasts. The benefit of incorporating EPL-MA and, interestingly, the stiffness of 3D-printed hydrogel on cell performance has been confirmed by various biological evaluations, including viability, adhesion, growth, and function of NIH/3T3 fibroblasts. Taken together, we have developed a rather promising strategy using photocurable epsilon-poly-L-lysine for promoting cell adhesion and functionalisation with potential for skin tissue engineering and other regenerative medicine applications.},
	urldate = {2023-09-07},
	journal = {EUROPEAN POLYMER JOURNAL},
	author = {Lertwimol, Tareerat and Sungkhaphan, Piyarat and Uppanan, Paweena and Zhang, Qiang and Haddleton, David M. and Risangud, Nuttapol},
	month = sep,
	year = {2023},
}

{"_id":"ZRDD6oLpPfCyXmn9k","bibbaseid":"lertwimol-sungkhaphan-uppanan-zhang-haddleton-risangud-afacilestrategyforpromotingcelladhesionandfunctiononthreedimensionalprintedhydrogelsusingphotocurableepsilonpolyllysine-2023","author_short":["Lertwimol, T.","Sungkhaphan, P.","Uppanan, P.","Zhang, Q.","Haddleton, D. M.","Risangud, N."],"bibdata":{"bibtype":"article","type":"article","title":"A facile strategy for promoting cell adhesion and function on three-dimensional printed hydrogels using photocurable epsilon-poly-L-lysine","volume":"196","issn":"0014-3057","doi":"10.1016/j.eurpolymj.2023.112245","abstract":"This study introduces a new persuasive strategy for promoting cell adhesion and function on three-dimensional (3D) printed hydrogels by incorporating photocurable epsilon-poly-L-lysine (EPL) as the cell adhesive molecule. Two different polymers, dextran (Dex) and EPL, were first modified using methacrylic anhydride and glycidyl methacrylate, respectively, to obtain the methacrylate-functionalized polymers: Dex-MA and EPL-MA. Subsequently, these modified polymers were formulated with poly(ethylene glycol dimethacrylate) (PEGDMA) before fabricating the 3D-printed hydrogels through digital light processing (DLP). The effects of the PEGDMA, Dex-MA, and EPL-MA ratios in the formulation on the physical properties of 3D-printed hydrogel and their biological responses were assessed. The 3D-printed specimens possessed compressive moduli in the range of 280-520 kPa. SEM/EDS analysis confirmed the presence of nitrogen in the hydrogels. The ninhydrin assay revealed that the EPL-MA content fell in the range of 0-0.15 mg/mg. All prepared hydrogels exhibited non-cytotoxic behavior toward NIH/3T3 fibroblasts. The benefit of incorporating EPL-MA and, interestingly, the stiffness of 3D-printed hydrogel on cell performance has been confirmed by various biological evaluations, including viability, adhesion, growth, and function of NIH/3T3 fibroblasts. Taken together, we have developed a rather promising strategy using photocurable epsilon-poly-L-lysine for promoting cell adhesion and functionalisation with potential for skin tissue engineering and other regenerative medicine applications.","urldate":"2023-09-07","journal":"EUROPEAN POLYMER JOURNAL","author":[{"propositions":[],"lastnames":["Lertwimol"],"firstnames":["Tareerat"],"suffixes":[]},{"propositions":[],"lastnames":["Sungkhaphan"],"firstnames":["Piyarat"],"suffixes":[]},{"propositions":[],"lastnames":["Uppanan"],"firstnames":["Paweena"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Qiang"],"suffixes":[]},{"propositions":[],"lastnames":["Haddleton"],"firstnames":["David","M."],"suffixes":[]},{"propositions":[],"lastnames":["Risangud"],"firstnames":["Nuttapol"],"suffixes":[]}],"month":"September","year":"2023","bibtex":"@article{lertwimol_facile_2023,\n\ttitle = {A facile strategy for promoting cell adhesion and function on three-dimensional printed hydrogels using photocurable epsilon-poly-{L}-lysine},\n\tvolume = {196},\n\tissn = {0014-3057},\n\tdoi = {10.1016/j.eurpolymj.2023.112245},\n\tabstract = {This study introduces a new persuasive strategy for promoting cell adhesion and function on three-dimensional (3D) printed hydrogels by incorporating photocurable epsilon-poly-L-lysine (EPL) as the cell adhesive molecule. Two different polymers, dextran (Dex) and EPL, were first modified using methacrylic anhydride and glycidyl methacrylate, respectively, to obtain the methacrylate-functionalized polymers: Dex-MA and EPL-MA. Subsequently, these modified polymers were formulated with poly(ethylene glycol dimethacrylate) (PEGDMA) before fabricating the 3D-printed hydrogels through digital light processing (DLP). The effects of the PEGDMA, Dex-MA, and EPL-MA ratios in the formulation on the physical properties of 3D-printed hydrogel and their biological responses were assessed. The 3D-printed specimens possessed compressive moduli in the range of 280-520 kPa. SEM/EDS analysis confirmed the presence of nitrogen in the hydrogels. The ninhydrin assay revealed that the EPL-MA content fell in the range of 0-0.15 mg/mg. All prepared hydrogels exhibited non-cytotoxic behavior toward NIH/3T3 fibroblasts. The benefit of incorporating EPL-MA and, interestingly, the stiffness of 3D-printed hydrogel on cell performance has been confirmed by various biological evaluations, including viability, adhesion, growth, and function of NIH/3T3 fibroblasts. Taken together, we have developed a rather promising strategy using photocurable epsilon-poly-L-lysine for promoting cell adhesion and functionalisation with potential for skin tissue engineering and other regenerative medicine applications.},\n\turldate = {2023-09-07},\n\tjournal = {EUROPEAN POLYMER JOURNAL},\n\tauthor = {Lertwimol, Tareerat and Sungkhaphan, Piyarat and Uppanan, Paweena and Zhang, Qiang and Haddleton, David M. and Risangud, Nuttapol},\n\tmonth = sep,\n\tyear = {2023},\n}\n\n","author_short":["Lertwimol, T.","Sungkhaphan, P.","Uppanan, P.","Zhang, Q.","Haddleton, D. M.","Risangud, N."],"key":"lertwimol_facile_2023","id":"lertwimol_facile_2023","bibbaseid":"lertwimol-sungkhaphan-uppanan-zhang-haddleton-risangud-afacilestrategyforpromotingcelladhesionandfunctiononthreedimensionalprintedhydrogelsusingphotocurableepsilonpolyllysine-2023","role":"author","urls":{},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://warwick.ac.uk/fac/cross_fac/wesic/research/wpg/research/WPGsep2024.bib","dataSources":["m4fxWT6XKrYfGbZFR","GBhqMC9hjyD5vqptk","6idaYK6okJY39mWgG"],"keywords":[],"search_terms":["facile","strategy","promoting","cell","adhesion","function","three","dimensional","printed","hydrogels","using","photocurable","epsilon","poly","lysine","lertwimol","sungkhaphan","uppanan","zhang","haddleton","risangud"],"title":"A facile strategy for promoting cell adhesion and function on three-dimensional printed hydrogels using photocurable epsilon-poly-L-lysine","year":2023}