High-density detection of restriction-site-associated DNA markers for rapid mapping of mutated loci in Neurospora. Lewis, Z. A., Shiver, A. L., Stiffler, N., Miller, M. R., Johnson, E. A., & Selker, E. U. Genetics, 177(2):1163-71, 2007. Lewis, Zachary A Shiver, Anthony L Stiffler, Nicholas Miller, Michael R Johnson, Eric A Selker, Eric U GM025690-22/GM/NIGMS NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United States 2007/07/31 Genetics. 2007 Oct;177(2):1163-71. doi: 10.1534/genetics.107.078147. Epub 2007 Jul 29.doi abstract bibtex The wealth of sequence information available for Neurospora crassa and other fungi has greatly facilitated evolutionary and molecular analyses of this group. Although "reverse" genetics, in which genes are first identified by their sequence rather than by their mutant phenotypes, serves as a valuable new approach for elucidating biological processes, classical "forward" genetic analysis is still extremely useful. Unfortunately, mapping mutations and identifying the corresponding genes has typically been slow and laborious. To facilitate forward genetics in Neurospora, we have adapted microarray-based restriction-site-associated DNA (RAD) mapping for use with N. crassa oligonucleotide microarrays. This technique was used to simultaneously detect an unprecedented number of genomewide restriction site polymorphisms from two N. crassa strains: Mauriceville and Oak Ridge. Furthermore, RAD mapping was used to quickly map a previously unknown gene, defective in methylation-7 (dim-7).
@article{RN50,
author = {Lewis, Z. A. and Shiver, A. L. and Stiffler, N. and Miller, M. R. and Johnson, E. A. and Selker, E. U.},
title = {High-density detection of restriction-site-associated DNA markers for rapid mapping of mutated loci in Neurospora},
journal = {Genetics},
volume = {177},
number = {2},
pages = {1163-71},
note = {Lewis, Zachary A
Shiver, Anthony L
Stiffler, Nicholas
Miller, Michael R
Johnson, Eric A
Selker, Eric U
GM025690-22/GM/NIGMS NIH HHS/United States
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
United States
2007/07/31
Genetics. 2007 Oct;177(2):1163-71. doi: 10.1534/genetics.107.078147. Epub 2007 Jul 29.},
abstract = {The wealth of sequence information available for Neurospora crassa and other fungi has greatly facilitated evolutionary and molecular analyses of this group. Although "reverse" genetics, in which genes are first identified by their sequence rather than by their mutant phenotypes, serves as a valuable new approach for elucidating biological processes, classical "forward" genetic analysis is still extremely useful. Unfortunately, mapping mutations and identifying the corresponding genes has typically been slow and laborious. To facilitate forward genetics in Neurospora, we have adapted microarray-based restriction-site-associated DNA (RAD) mapping for use with N. crassa oligonucleotide microarrays. This technique was used to simultaneously detect an unprecedented number of genomewide restriction site polymorphisms from two N. crassa strains: Mauriceville and Oak Ridge. Furthermore, RAD mapping was used to quickly map a previously unknown gene, defective in methylation-7 (dim-7).},
keywords = {DNA, Fungal/*genetics
Genes, Fungal
Genetic Markers
Genome, Fungal/*genetics
Microarray Analysis
Mutation
Neurospora crassa/*genetics
Polymorphism, Genetic
Restriction Mapping/*methods},
ISSN = {0016-6731 (Print)
0016-6731},
DOI = {10.1534/genetics.107.078147},
year = {2007},
type = {Journal Article}
}
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