@article{li_comparison_2016,
	title = {Comparison of liver {microRNA} transcriptomes of {Tibetan} and {Yorkshire} pigs by deep sequencing},
	volume = {577},
	issn = {1879-0038},
	doi = {10.1016/j.gene.2015.12.003},
	abstract = {MicroRNAs (miRNAs) play an important role in the modulation of various metabolic processes in the liver, yet little is known about the liver microRNAome (miRNAome) of the Tibetan pig. Here we used the Yorkshire pig as a control to analyze the Tibetan pig-specific liver miRNAome, and for preliminary investigation of differentially expressed miRNAs participating in metabolism. A comprehensive analysis of Tibetan and Yorkshire pig liver miRNAomes by small RNA sequencing identified 362 unique miRNAs. Among these, 304 were co-expressed in both libraries, and 10 and 48 miRNAs were specifically expressed. Differential expression analysis of miRNAs, miRNA target prediction and KEGG analysis revealed that differentially expressed miRNAs were associated mainly with the metabolism of glucose, lipid and protein. Six differentially expressed miRNAs (miR-34a, miR-326, miR-1, miR-335, miR-185 and miR-378) participating in the metabolism of glucose and lipid were identified. Additionally, qPCR results revealed that a lower expression of miR-34a in Tibetan pig liver may promote gluconeogenesis by increasing the expression of Sirtuin type 1 (Sirt1); a lower expression of miR-1 in Tibetan pig liver may promote the synthesis and accumulation of lipid by increasing the expression of Liver X receptor α (LXRα); and a lower expression of miR-185 in Tibetan pig liver may promote the uptake of cholesterol from blood and secretion of bile by increasing the expression of the scavenger receptor class B type I (SR-BI). Our results provide new information and understanding of porcine miRNA profiles, which may help explain the regulatory mechanisms of miRNAs in the metabolic functions of Tibetan pig liver, and provide new biomarkers to assist in the development of Tibetan pig breeding characteristics.},
	language = {eng},
	number = {2},
	journal = {Gene},
	author = {Li, Yanyue and Li, Xiaocheng and Sun, Wen-Kui and Cheng, Chi and Chen, Yi-Hui and Zeng, Kai and Chen, Xiaohui and Gu, Yiren and Gao, Rong and Liu, Rui and Lv, Xuebin},
	month = feb,
	year = {2016},
	pmid = {26656174},
	keywords = {Animals, Base Sequence, Liver, MiRNAome, MiRNAs, MicroRNAs, Molecular Sequence Data, Sequence Alignment, Sus scrofa, Tibetan pig, Transcriptome, Yorkshire pig},
	pages = {244--250},
}

{"_id":"2HbZkZEbogdYBoKtG","bibbaseid":"li-li-sun-cheng-chen-zeng-chen-gu-etal-comparisonoflivermicrornatranscriptomesoftibetanandyorkshirepigsbydeepsequencing-2016","downloads":0,"creationDate":"2019-03-08T19:02:45.740Z","title":"Comparison of liver microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing","author_short":["Li, Y.","Li, X.","Sun, W.","Cheng, C.","Chen, Y.","Zeng, K.","Chen, X.","Gu, Y.","Gao, R.","Liu, R.","Lv, X."],"year":2016,"bibtype":"article","biburl":"https://bibbase.org/zotero/sannpeterson","bibdata":{"bibtype":"article","type":"article","title":"Comparison of liver microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing","volume":"577","issn":"1879-0038","doi":"10.1016/j.gene.2015.12.003","abstract":"MicroRNAs (miRNAs) play an important role in the modulation of various metabolic processes in the liver, yet little is known about the liver microRNAome (miRNAome) of the Tibetan pig. Here we used the Yorkshire pig as a control to analyze the Tibetan pig-specific liver miRNAome, and for preliminary investigation of differentially expressed miRNAs participating in metabolism. A comprehensive analysis of Tibetan and Yorkshire pig liver miRNAomes by small RNA sequencing identified 362 unique miRNAs. Among these, 304 were co-expressed in both libraries, and 10 and 48 miRNAs were specifically expressed. Differential expression analysis of miRNAs, miRNA target prediction and KEGG analysis revealed that differentially expressed miRNAs were associated mainly with the metabolism of glucose, lipid and protein. Six differentially expressed miRNAs (miR-34a, miR-326, miR-1, miR-335, miR-185 and miR-378) participating in the metabolism of glucose and lipid were identified. Additionally, qPCR results revealed that a lower expression of miR-34a in Tibetan pig liver may promote gluconeogenesis by increasing the expression of Sirtuin type 1 (Sirt1); a lower expression of miR-1 in Tibetan pig liver may promote the synthesis and accumulation of lipid by increasing the expression of Liver X receptor α (LXRα); and a lower expression of miR-185 in Tibetan pig liver may promote the uptake of cholesterol from blood and secretion of bile by increasing the expression of the scavenger receptor class B type I (SR-BI). Our results provide new information and understanding of porcine miRNA profiles, which may help explain the regulatory mechanisms of miRNAs in the metabolic functions of Tibetan pig liver, and provide new biomarkers to assist in the development of Tibetan pig breeding characteristics.","language":"eng","number":"2","journal":"Gene","author":[{"propositions":[],"lastnames":["Li"],"firstnames":["Yanyue"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Xiaocheng"],"suffixes":[]},{"propositions":[],"lastnames":["Sun"],"firstnames":["Wen-Kui"],"suffixes":[]},{"propositions":[],"lastnames":["Cheng"],"firstnames":["Chi"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Yi-Hui"],"suffixes":[]},{"propositions":[],"lastnames":["Zeng"],"firstnames":["Kai"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Xiaohui"],"suffixes":[]},{"propositions":[],"lastnames":["Gu"],"firstnames":["Yiren"],"suffixes":[]},{"propositions":[],"lastnames":["Gao"],"firstnames":["Rong"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Rui"],"suffixes":[]},{"propositions":[],"lastnames":["Lv"],"firstnames":["Xuebin"],"suffixes":[]}],"month":"February","year":"2016","pmid":"26656174","keywords":"Animals, Base Sequence, Liver, MiRNAome, MiRNAs, MicroRNAs, Molecular Sequence Data, Sequence Alignment, Sus scrofa, Tibetan pig, Transcriptome, Yorkshire pig","pages":"244–250","bibtex":"@article{li_comparison_2016,\n\ttitle = {Comparison of liver {microRNA} transcriptomes of {Tibetan} and {Yorkshire} pigs by deep sequencing},\n\tvolume = {577},\n\tissn = {1879-0038},\n\tdoi = {10.1016/j.gene.2015.12.003},\n\tabstract = {MicroRNAs (miRNAs) play an important role in the modulation of various metabolic processes in the liver, yet little is known about the liver microRNAome (miRNAome) of the Tibetan pig. Here we used the Yorkshire pig as a control to analyze the Tibetan pig-specific liver miRNAome, and for preliminary investigation of differentially expressed miRNAs participating in metabolism. A comprehensive analysis of Tibetan and Yorkshire pig liver miRNAomes by small RNA sequencing identified 362 unique miRNAs. Among these, 304 were co-expressed in both libraries, and 10 and 48 miRNAs were specifically expressed. Differential expression analysis of miRNAs, miRNA target prediction and KEGG analysis revealed that differentially expressed miRNAs were associated mainly with the metabolism of glucose, lipid and protein. Six differentially expressed miRNAs (miR-34a, miR-326, miR-1, miR-335, miR-185 and miR-378) participating in the metabolism of glucose and lipid were identified. Additionally, qPCR results revealed that a lower expression of miR-34a in Tibetan pig liver may promote gluconeogenesis by increasing the expression of Sirtuin type 1 (Sirt1); a lower expression of miR-1 in Tibetan pig liver may promote the synthesis and accumulation of lipid by increasing the expression of Liver X receptor α (LXRα); and a lower expression of miR-185 in Tibetan pig liver may promote the uptake of cholesterol from blood and secretion of bile by increasing the expression of the scavenger receptor class B type I (SR-BI). Our results provide new information and understanding of porcine miRNA profiles, which may help explain the regulatory mechanisms of miRNAs in the metabolic functions of Tibetan pig liver, and provide new biomarkers to assist in the development of Tibetan pig breeding characteristics.},\n\tlanguage = {eng},\n\tnumber = {2},\n\tjournal = {Gene},\n\tauthor = {Li, Yanyue and Li, Xiaocheng and Sun, Wen-Kui and Cheng, Chi and Chen, Yi-Hui and Zeng, Kai and Chen, Xiaohui and Gu, Yiren and Gao, Rong and Liu, Rui and Lv, Xuebin},\n\tmonth = feb,\n\tyear = {2016},\n\tpmid = {26656174},\n\tkeywords = {Animals, Base Sequence, Liver, MiRNAome, MiRNAs, MicroRNAs, Molecular Sequence Data, Sequence Alignment, Sus scrofa, Tibetan pig, Transcriptome, Yorkshire pig},\n\tpages = {244--250},\n}\n\n","author_short":["Li, Y.","Li, X.","Sun, W.","Cheng, C.","Chen, Y.","Zeng, K.","Chen, X.","Gu, Y.","Gao, R.","Liu, R.","Lv, X."],"key":"li_comparison_2016","id":"li_comparison_2016","bibbaseid":"li-li-sun-cheng-chen-zeng-chen-gu-etal-comparisonoflivermicrornatranscriptomesoftibetanandyorkshirepigsbydeepsequencing-2016","role":"author","urls":{},"keyword":["Animals","Base Sequence","Liver","MiRNAome","MiRNAs","MicroRNAs","Molecular Sequence Data","Sequence Alignment","Sus scrofa","Tibetan pig","Transcriptome","Yorkshire pig"],"metadata":{"authorlinks":{}},"html":""},"search_terms":["comparison","liver","microrna","transcriptomes","tibetan","yorkshire","pigs","deep","sequencing","li","li","sun","cheng","chen","zeng","chen","gu","gao","liu","lv"],"keywords":["animals","base sequence","liver","mirnaome","mirnas","micrornas","molecular sequence data","sequence alignment","sus scrofa","tibetan pig","transcriptome","yorkshire pig"],"authorIDs":[],"dataSources":["JPAoq8b4ppzXj5XYZ"]}