Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel: Results from a 2005–2008 survey. Li, H., Toubiana, M., Monfort, P., & Roch, P. 33(9):974–979. Number: 9![Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel: Results from a 2005–2008 survey [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Several bivalves, including mussels, suffered from mortalities particularly in summer. To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-PCR was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and HSP70. House keeping gene was 28S rRNA. Defensin, myticin B and lysozyme appeared more expressed in spring–summer than in winter. In contrast, HSP70 expression was higher in winter. Statistical studies using principal component analysis (PCA) and multiple regression models revealed positive influence of temperature on 28S rRNA, defensin, myticin B and lysozyme expressions, but not on mytilin B and HSP70. The positive influence was significant for defensin and lysozyme expression, but relationships cannot be quantified. Similarly, salinity appeared to influence defensin expression, but this relationship cannot be quantified neither. E. coli tissue content appeared without influence. Consequently, there was no clear relationship between environmental parameters and immune-related gene expressions, demonstrating anti-infectious capabilities cannot be evaluated using only the expression of such genes as markers.
@article{li_influence_2009,
title = {Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel: Results from a 2005–2008 survey},
volume = {33},
issn = {0145-305X},
url = {http://www.sciencedirect.com/science/article/pii/S0145305X09000858},
doi = {10.1016/j.dci.2009.04.002},
shorttitle = {Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel},
abstract = {Several bivalves, including mussels, suffered from mortalities particularly in summer. To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-{PCR} was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and {HSP}70. House keeping gene was 28S {rRNA}. Defensin, myticin B and lysozyme appeared more expressed in spring–summer than in winter. In contrast, {HSP}70 expression was higher in winter. Statistical studies using principal component analysis ({PCA}) and multiple regression models revealed positive influence of temperature on 28S {rRNA}, defensin, myticin B and lysozyme expressions, but not on mytilin B and {HSP}70. The positive influence was significant for defensin and lysozyme expression, but relationships cannot be quantified. Similarly, salinity appeared to influence defensin expression, but this relationship cannot be quantified neither. E. coli tissue content appeared without influence. Consequently, there was no clear relationship between environmental parameters and immune-related gene expressions, demonstrating anti-infectious capabilities cannot be evaluated using only the expression of such genes as markers.},
pages = {974--979},
number = {9},
journaltitle = {Developmental \& Comparative Immunology},
shortjournal = {Developmental \& Comparative Immunology},
author = {Li, Hui and Toubiana, Mylène and Monfort, Patrick and Roch, Philippe},
urldate = {2019-04-16},
date = {2009-09-01},
note = {Number: 9},
keywords = {Antimicrobial peptide, Immune survey, Innate immunity, Mollusks, Q-{PCR}, Seasonal variations}
}
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To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-PCR was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and HSP70. House keeping gene was 28S rRNA. Defensin, myticin B and lysozyme appeared more expressed in spring–summer than in winter. In contrast, HSP70 expression was higher in winter. Statistical studies using principal component analysis (PCA) and multiple regression models revealed positive influence of temperature on 28S rRNA, defensin, myticin B and lysozyme expressions, but not on mytilin B and HSP70. The positive influence was significant for defensin and lysozyme expression, but relationships cannot be quantified. Similarly, salinity appeared to influence defensin expression, but this relationship cannot be quantified neither. E. coli tissue content appeared without influence. 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To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-{PCR} was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and {HSP}70. House keeping gene was 28S {rRNA}. Defensin, myticin B and lysozyme appeared more expressed in spring–summer than in winter. In contrast, {HSP}70 expression was higher in winter. Statistical studies using principal component analysis ({PCA}) and multiple regression models revealed positive influence of temperature on 28S {rRNA}, defensin, myticin B and lysozyme expressions, but not on mytilin B and {HSP}70. The positive influence was significant for defensin and lysozyme expression, but relationships cannot be quantified. 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