@Article{Li2009,
  author   = {Li, Qifeng and Wu, Sherry and Chou, Keng C.},
  journal  = {Biophysical Journal},
  title    = {Subdiffraction-{Limit} {Two}-{Photon} {Fluorescence} {Microscopy} for {GFP}-{Tagged} {Cell} {Imaging}},
  year     = {2009},
  issn     = {0006-3495},
  month    = dec,
  number   = {12},
  pages    = {3224--3228},
  volume   = {97},
  abstract = {We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intrinsic three-dimensional resolution, confined photobleaching, and subdiffraction-limit resolution. The subdiffraction-limit resolution was demonstrated by resolving green-fluorescent-protein-tagged caveolar vesicles located within a distance shorter than the diffraction limit of a regular 2PEF microscope, which is ~250 nm even with the best optics. The full width at half-maximum of the effective point-spread function for the 2PEF microscope was estimated to be ~54 nm.},
  doi      = {10.1016/j.bpj.2009.09.038},
  file     = {ScienceDirect Snapshot:https\://www.sciencedirect.com/science/article/pii/S0006349509015227:text/html;ScienceDirect Full Text PDF:https\://pdf.sciencedirectassets.com/277708/1-s2.0-S0006349509X00251/1-s2.0-S0006349509015227/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEIf%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIBenlPDlLsRMxtqdn0BmN0xVKUk1gwYs7NmQ%2F6hZ8T8%2FAiEAiHVU1vcfPdcAHoMphFZTEoGvfNYy3b7PDXnzA9ljfwcqvQMI%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARADGgwwNTkwMDM1NDY4NjUiDLXardf%2B%2Fy00yJW01CqRA2mF0RdfRRzpGxsY6WP143AZLS2am4MuSfD%2BlUApXVUT2MGcxNlPkEut2a5MbMan%2FY7rlTfoB07bKRFAslCxcG5Xg9TcHf4VwXai6eaKV4%2BvQS1%2Bbg7KXTRcX%2BkzAxlod95VGgxGbGxoWN5X2ncDZVY4pflC9m6ll0UNGDWqDJdCiGiRltk88m9iUCocZOnhmXpC6WmBLEHeGxXCg%2Fath6RhxJKYMTFbPe04e6zTVA1CvibtsgmDl2HfqpTy4HTW3WV20w2SgsDQBb%2B8HF6FDlpsxBHB%2BPF90sY%2FtQ3s8JhShWg%2B37GPTWGJJfr5oR4392tv8r52OyK%2F%2BMYTs0d2S6aBPE52wh0SW7ZUmeaNRcGhC9vJciX%2FDEyiPFx4CuM7pxYhMrrEcfKBM%2BUI%2FiWfk01qpycshhxrr2fgInnLAAtXf82sh%2Bdo1lKECt4wKDX%2BXMRJQKHsELKE3xM6fCpcW%2BlFyHE4gDrjCexO01LJNBAuR5BFeJY4bJOYknFKRw0pLbda%2BAqsLblLjSo0hwuA%2BV2aMOursfcFOusBwfLwLOm0AZULsY5bWVkH%2FjMvM3Stke%2FC1Bio9EhqNWoeBYK8ZlUh1mMcOLR8rgg77iHkb1B18CsOr9DThQMjhQzZ7KBGsl0faQe0l%2BpSv8qFQmaehBRNJwwkwk2ANqXonWlj%2FozqxiurXCkjLMQDx5Ds%2FqyvXlaaDIK9GQnr1qCajNiuibJq7xTHb4O25NVpOLRJCiJJVZxoKWsBDFrr2rrJiH7b2STND6sReFcrY4kf0wwW5Ewkwq2RPhIIlGAup6Cot7Psv5YWiJ1rPPW7P8FcbAhPgcfck5Suu%2B5CrmugWOR%2Br6wNP%2FXOSA%3D%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20200619T070549Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY7HVYNWS2%2F20200619%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=a2e269b9b56d3b61d500a2964095136268187b3f7933bf1c3d9de6a58d57a0e2&hash=96908f727f5d979092b2d257ba42a6f65ac4cc760123cb45a798ec599f97ab05&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0006349509015227&tid=spdf-4c205679-4bd1-4940-b2a3-32aa977e6e91&sid=16465ada24b5d3458249325-7c62a3a45016gxrqa&type=client:application/pdf},
  language = {en},
  url      = {http://www.sciencedirect.com/science/article/pii/S0006349509015227},
  urldate  = {2020-06-19TZ},
}

{"_id":"oJW9LAtN38SioDQW8","bibbaseid":"li-wu-chou-subdiffractionlimittwophotonfluorescencemicroscopyforgfptaggedcellimaging-2009","authorIDs":[],"author_short":["Li, Q.","Wu, S.","Chou, K. C."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Li"],"firstnames":["Qifeng"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["Sherry"],"suffixes":[]},{"propositions":[],"lastnames":["Chou"],"firstnames":["Keng","C."],"suffixes":[]}],"journal":"Biophysical Journal","title":"Subdiffraction-Limit Two-Photon Fluorescence Microscopy for GFP-Tagged Cell Imaging","year":"2009","issn":"0006-3495","month":"December","number":"12","pages":"3224–3228","volume":"97","abstract":"We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intrinsic three-dimensional resolution, confined photobleaching, and subdiffraction-limit resolution. The subdiffraction-limit resolution was demonstrated by resolving green-fluorescent-protein-tagged caveolar vesicles located within a distance shorter than the diffraction limit of a regular 2PEF microscope, which is ~250 nm even with the best optics. The full width at half-maximum of the effective point-spread function for the 2PEF microscope was estimated to be ~54 nm.","doi":"10.1016/j.bpj.2009.09.038","file":"ScienceDirect Snapshot:https\\://www.sciencedirect.com/science/article/pii/S0006349509015227:text/html;ScienceDirect Full Text PDF:https\\://pdf.sciencedirectassets.com/277708/1-s2.0-S0006349509X00251/1-s2.0-S0006349509015227/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEIf%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIBenlPDlLsRMxtqdn0BmN0xVKUk1gwYs7NmQ%2F6hZ8T8%2FAiEAiHVU1vcfPdcAHoMphFZTEoGvfNYy3b7PDXnzA9ljfwcqvQMI%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARADGgwwNTkwMDM1NDY4NjUiDLXardf%2B%2Fy00yJW01CqRA2mF0RdfRRzpGxsY6WP143AZLS2am4MuSfD%2BlUApXVUT2MGcxNlPkEut2a5MbMan%2FY7rlTfoB07bKRFAslCxcG5Xg9TcHf4VwXai6eaKV4%2BvQS1%2Bbg7KXTRcX%2BkzAxlod95VGgxGbGxoWN5X2ncDZVY4pflC9m6ll0UNGDWqDJdCiGiRltk88m9iUCocZOnhmXpC6WmBLEHeGxXCg%2Fath6RhxJKYMTFbPe04e6zTVA1CvibtsgmDl2HfqpTy4HTW3WV20w2SgsDQBb%2B8HF6FDlpsxBHB%2BPF90sY%2FtQ3s8JhShWg%2B37GPTWGJJfr5oR4392tv8r52OyK%2F%2BMYTs0d2S6aBPE52wh0SW7ZUmeaNRcGhC9vJciX%2FDEyiPFx4CuM7pxYhMrrEcfKBM%2BUI%2FiWfk01qpycshhxrr2fgInnLAAtXf82sh%2Bdo1lKECt4wKDX%2BXMRJQKHsELKE3xM6fCpcW%2BlFyHE4gDrjCexO01LJNBAuR5BFeJY4bJOYknFKRw0pLbda%2BAqsLblLjSo0hwuA%2BV2aMOursfcFOusBwfLwLOm0AZULsY5bWVkH%2FjMvM3Stke%2FC1Bio9EhqNWoeBYK8ZlUh1mMcOLR8rgg77iHkb1B18CsOr9DThQMjhQzZ7KBGsl0faQe0l%2BpSv8qFQmaehBRNJwwkwk2ANqXonWlj%2FozqxiurXCkjLMQDx5Ds%2FqyvXlaaDIK9GQnr1qCajNiuibJq7xTHb4O25NVpOLRJCiJJVZxoKWsBDFrr2rrJiH7b2STND6sReFcrY4kf0wwW5Ewkwq2RPhIIlGAup6Cot7Psv5YWiJ1rPPW7P8FcbAhPgcfck5Suu%2B5CrmugWOR%2Br6wNP%2FXOSA%3D%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20200619T070549Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY7HVYNWS2%2F20200619%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=a2e269b9b56d3b61d500a2964095136268187b3f7933bf1c3d9de6a58d57a0e2&hash=96908f727f5d979092b2d257ba42a6f65ac4cc760123cb45a798ec599f97ab05&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0006349509015227&tid=spdf-4c205679-4bd1-4940-b2a3-32aa977e6e91&sid=16465ada24b5d3458249325-7c62a3a45016gxrqa&type=client:application/pdf","language":"en","url":"http://www.sciencedirect.com/science/article/pii/S0006349509015227","urldate":"2020-06-19TZ","bibtex":"@Article{Li2009,\r\n author = {Li, Qifeng and Wu, Sherry and Chou, Keng C.},\r\n journal = {Biophysical Journal},\r\n title = {Subdiffraction-{Limit} {Two}-{Photon} {Fluorescence} {Microscopy} for {GFP}-{Tagged} {Cell} {Imaging}},\r\n year = {2009},\r\n issn = {0006-3495},\r\n month = dec,\r\n number = {12},\r\n pages = {3224--3228},\r\n volume = {97},\r\n abstract = {We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intrinsic three-dimensional resolution, confined photobleaching, and subdiffraction-limit resolution. The subdiffraction-limit resolution was demonstrated by resolving green-fluorescent-protein-tagged caveolar vesicles located within a distance shorter than the diffraction limit of a regular 2PEF microscope, which is ~250 nm even with the best optics. The full width at half-maximum of the effective point-spread function for the 2PEF microscope was estimated to be ~54 nm.},\r\n doi = {10.1016/j.bpj.2009.09.038},\r\n file = {ScienceDirect Snapshot:https\\://www.sciencedirect.com/science/article/pii/S0006349509015227:text/html;ScienceDirect Full Text PDF:https\\://pdf.sciencedirectassets.com/277708/1-s2.0-S0006349509X00251/1-s2.0-S0006349509015227/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEIf%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIBenlPDlLsRMxtqdn0BmN0xVKUk1gwYs7NmQ%2F6hZ8T8%2FAiEAiHVU1vcfPdcAHoMphFZTEoGvfNYy3b7PDXnzA9ljfwcqvQMI%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARADGgwwNTkwMDM1NDY4NjUiDLXardf%2B%2Fy00yJW01CqRA2mF0RdfRRzpGxsY6WP143AZLS2am4MuSfD%2BlUApXVUT2MGcxNlPkEut2a5MbMan%2FY7rlTfoB07bKRFAslCxcG5Xg9TcHf4VwXai6eaKV4%2BvQS1%2Bbg7KXTRcX%2BkzAxlod95VGgxGbGxoWN5X2ncDZVY4pflC9m6ll0UNGDWqDJdCiGiRltk88m9iUCocZOnhmXpC6WmBLEHeGxXCg%2Fath6RhxJKYMTFbPe04e6zTVA1CvibtsgmDl2HfqpTy4HTW3WV20w2SgsDQBb%2B8HF6FDlpsxBHB%2BPF90sY%2FtQ3s8JhShWg%2B37GPTWGJJfr5oR4392tv8r52OyK%2F%2BMYTs0d2S6aBPE52wh0SW7ZUmeaNRcGhC9vJciX%2FDEyiPFx4CuM7pxYhMrrEcfKBM%2BUI%2FiWfk01qpycshhxrr2fgInnLAAtXf82sh%2Bdo1lKECt4wKDX%2BXMRJQKHsELKE3xM6fCpcW%2BlFyHE4gDrjCexO01LJNBAuR5BFeJY4bJOYknFKRw0pLbda%2BAqsLblLjSo0hwuA%2BV2aMOursfcFOusBwfLwLOm0AZULsY5bWVkH%2FjMvM3Stke%2FC1Bio9EhqNWoeBYK8ZlUh1mMcOLR8rgg77iHkb1B18CsOr9DThQMjhQzZ7KBGsl0faQe0l%2BpSv8qFQmaehBRNJwwkwk2ANqXonWlj%2FozqxiurXCkjLMQDx5Ds%2FqyvXlaaDIK9GQnr1qCajNiuibJq7xTHb4O25NVpOLRJCiJJVZxoKWsBDFrr2rrJiH7b2STND6sReFcrY4kf0wwW5Ewkwq2RPhIIlGAup6Cot7Psv5YWiJ1rPPW7P8FcbAhPgcfck5Suu%2B5CrmugWOR%2Br6wNP%2FXOSA%3D%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20200619T070549Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY7HVYNWS2%2F20200619%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=a2e269b9b56d3b61d500a2964095136268187b3f7933bf1c3d9de6a58d57a0e2&hash=96908f727f5d979092b2d257ba42a6f65ac4cc760123cb45a798ec599f97ab05&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0006349509015227&tid=spdf-4c205679-4bd1-4940-b2a3-32aa977e6e91&sid=16465ada24b5d3458249325-7c62a3a45016gxrqa&type=client:application/pdf},\r\n language = {en},\r\n url = {http://www.sciencedirect.com/science/article/pii/S0006349509015227},\r\n urldate = {2020-06-19TZ},\r\n}\r\n\r\n","author_short":["Li, Q.","Wu, S.","Chou, K. C."],"key":"Li2009","id":"Li2009","bibbaseid":"li-wu-chou-subdiffractionlimittwophotonfluorescencemicroscopyforgfptaggedcellimaging-2009","role":"author","urls":{"Paper":"http://www.sciencedirect.com/science/article/pii/S0006349509015227"},"downloads":0,"html":""},"bibtype":"article","biburl":"https://lasir.ca/wp-content/uploads/LASIR-biblio.bib","creationDate":"2020-07-24T08:03:38.808Z","downloads":0,"keywords":[],"search_terms":["subdiffraction","limit","two","photon","fluorescence","microscopy","gfp","tagged","cell","imaging","li","wu","chou"],"title":"Subdiffraction-Limit Two-Photon Fluorescence Microscopy for GFP-Tagged Cell Imaging","year":2009,"dataSources":["EwrBFmJGvAFRq9Qaq"]}