Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning. Lindström, N., Tran, T, Guo, J, Rutledge, E, Parvez, R., Thornton, M., Grubbs, B, McMahon, J., & McMahon, A. Journal of the American Society of Nephrology, February, 2018.
doi  abstract   bibtex   
The nephron is the functional unit of the kidney, but the mechanism of nephron formation during human development is unclear. We conducted a detailed analysis of nephron development in humans and mice by immunolabeling, and we compared human and mouse nephron patterning to describe conserved and divergent features. We created protein localization maps that highlight the emerging patterns along the proximal–distal axis of the developing nephron and benchmark expectations for localization of functionally important transcription factors, which revealed unanticipated cellular diversity. Moreover, we identified a novel nephron subdomain marked by Wnt4 expression that we fate-mapped to the proximal mature nephron. Significant conservation was observed between human and mouse patterning. We also determined the time at which markers for mature nephron cell types first emerge—critical data for the renal organoid field. These findings have conceptual implications for the evolutionary processes driving the diversity of mammalian organ systems. Furthermore, these findings provide practical insights beyond those gained with mouse and rat models that will guide in vitro efforts to harness the developmental programs necessary to build human kidney structures.
@article{lindstrom_conserved_2018-2,
	title = {Conserved and {Divergent} {Molecular} and {Anatomic} {Features} of {Human} and {Mouse} {Nephron} {Patterning}},
	doi = {doi: 10.1681/ASN.2017091036},
	abstract = {The nephron is the functional unit of the kidney, but the mechanism of nephron formation during human development is unclear. We conducted a detailed analysis of nephron development in humans and mice by immunolabeling, and we compared human and mouse nephron patterning to describe conserved and divergent features. We created protein localization maps that highlight the emerging patterns along the proximal–distal axis of the developing nephron and benchmark expectations for localization of functionally important transcription factors, which revealed unanticipated cellular diversity. Moreover, we identified a novel nephron subdomain marked by Wnt4 expression that we fate-mapped to the proximal mature nephron. Significant conservation was observed between human and mouse patterning. We also determined the time at which markers for mature nephron cell types first emerge—critical data for the renal organoid field. These findings have conceptual implications for the evolutionary processes driving the diversity of mammalian organ systems. Furthermore, these findings provide practical insights beyond those gained with mouse and rat models that will guide in vitro efforts to harness the developmental programs necessary to build human kidney structures.},
	journal = {Journal of the American Society of Nephrology},
	author = {Lindström, NO and Tran, T and Guo, J and Rutledge, E and Parvez, RK and Thornton, ME and Grubbs, B and McMahon, JA and McMahon, AP},
	month = feb,
	year = {2018},
}
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