Fabrication and Impact of Fouling-Reducing Temperature-Responsive POEGMA Coatings with Embedded CaCO3 Nanoparticles on Different Cell Lines. Lishchynskyi, O., Stetsyshyn, Y., Raczkowska, J., Awsiuk, K., Orzechowska, B., Abalymov, A., Skirtach, A. G., Bernasik, A., Nastyshyn, S., & Budkowski, A. Materials, 14(6):1417, January, 2021. Number: 6 Publisher: Multidisciplinary Digital Publishing Institute
Fabrication and Impact of Fouling-Reducing Temperature-Responsive POEGMA Coatings with Embedded CaCO3 Nanoparticles on Different Cell Lines [link]Paper  doi  abstract   bibtex   
In the present work, we have successfully prepared and characterized novel nanocomposite material exhibiting temperature-dependent surface wettability changes, based on grafted brush coatings of non-fouling poly(di(ethylene glycol)methyl ether methacrylate) (POEGMA) with the embedded CaCO3 nanoparticles. Grafted polymer brushes attached to the glass surface were prepared in a three-step process using atom transfer radical polymerization (ATRP). Subsequently, uniform CaCO3 nanoparticles (NPs) embedded in POEGMA-grafted brush coatings were synthesized using biomineralized precipitation from solutions of CaCl2 and Na2CO3. An impact of the low concentration of the embedded CaCO3 NPs on cell adhesion and growth depends strongly on the type of studied cell line: keratinocytes (HaCaT), melanoma (WM35) and osteoblastic (MC3T3-e1). Based on the temperature-responsive properties of grafted brush coatings and CaCO3 NPs acting as biologically active substrate, we hope that our research will lead to a new platform for tissue engineering with modified growth of the cells due to the release of biologically active substances from CaCO3 NPs and the ability to detach the cells in a controlled manner using temperature-induced changes of the brush.
@article{lishchynskyi_fabrication_2021,
	title = {Fabrication and {Impact} of {Fouling}-{Reducing} {Temperature}-{Responsive} {POEGMA} {Coatings} with {Embedded} {CaCO3} {Nanoparticles} on {Different} {Cell} {Lines}},
	volume = {14},
	copyright = {http://creativecommons.org/licenses/by/3.0/},
	url = {https://www.mdpi.com/1996-1944/14/6/1417},
	doi = {10.3390/ma14061417},
	abstract = {In the present work, we have successfully prepared and characterized novel nanocomposite material exhibiting temperature-dependent surface wettability changes, based on grafted brush coatings of non-fouling poly(di(ethylene glycol)methyl ether methacrylate) (POEGMA) with the embedded CaCO3 nanoparticles. Grafted polymer brushes attached to the glass surface were prepared in a three-step process using atom transfer radical polymerization (ATRP). Subsequently, uniform CaCO3 nanoparticles (NPs) embedded in POEGMA-grafted brush coatings were synthesized using biomineralized precipitation from solutions of CaCl2 and Na2CO3. An impact of the low concentration of the embedded CaCO3 NPs on cell adhesion and growth depends strongly on the type of studied cell line: keratinocytes (HaCaT), melanoma (WM35) and osteoblastic (MC3T3-e1). Based on the temperature-responsive properties of grafted brush coatings and CaCO3 NPs acting as biologically active substrate, we hope that our research will lead to a new platform for tissue engineering with modified growth of the cells due to the release of biologically active substances from CaCO3 NPs and the ability to detach the cells in a controlled manner using temperature-induced changes of the brush.},
	language = {en},
	number = {6},
	urldate = {2021-09-27},
	journal = {Materials},
	author = {Lishchynskyi, Ostap and Stetsyshyn, Yurij and Raczkowska, Joanna and Awsiuk, Kamil and Orzechowska, Barbara and Abalymov, Anatolii and Skirtach, Andre G. and Bernasik, Andrzej and Nastyshyn, Svyatoslav and Budkowski, Andrzej},
	month = jan,
	year = {2021},
	note = {Number: 6
Publisher: Multidisciplinary Digital Publishing Institute},
	keywords = {HaCaT, MC3T3-e1, WM35, cell adhesion, cells, grafted coatings, nanoparticles, poly(di(ethylene glycol)methyl ether methacrylate)},
	pages = {1417},
}

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