Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c. Liu, X., Kim, C. N., Yang, J., Jemmerson, R., & Wang, X. Cell, 86(1):147–157, July, 1996.
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c [link]Paper  doi  abstract   bibtex   
A cell-free system based on cytosols of normally growing cells is established that reproduces aspects of the apoptotic program in vitro. The apoptotic program is initiated by addition of dATP. Fractionation of cytosol yielded a 15 kDa protein that is required for in vitro apoptosis. The absorption spectrum and protein sequence revealed that this protein is cytochrome c. Elimination of cytochrome c from cytosol by immunodepletion, or inclusion of sucrose to stabilize mitochondria during cytosol preparation, diminished the apoptotic activity. Adding back cytochrome c to the cytochrome c–depleted extracts restored their apoptotic activity. Cells undergoing apoptosis in vivo showed increased release of cytochrome c to their cytosol, suggesting that mitochondria may function in apoptosis by releasing cytochrome c.
@article{liu_induction_1996,
	title = {Induction of {Apoptotic} {Program} in {Cell}-{Free} {Extracts}: {Requirement} for {dATP} and {Cytochrome} c},
	volume = {86},
	issn = {0092-8674},
	shorttitle = {Induction of {Apoptotic} {Program} in {Cell}-{Free} {Extracts}},
	url = {https://www.sciencedirect.com/science/article/pii/S0092867400800859},
	doi = {10.1016/S0092-8674(00)80085-9},
	abstract = {A cell-free system based on cytosols of normally growing cells is established that reproduces aspects of the apoptotic program in vitro. The apoptotic program is initiated by addition of dATP. Fractionation of cytosol yielded a 15 kDa protein that is required for in vitro apoptosis. The absorption spectrum and protein sequence revealed that this protein is cytochrome c. Elimination of cytochrome c from cytosol by immunodepletion, or inclusion of sucrose to stabilize mitochondria during cytosol preparation, diminished the apoptotic activity. Adding back cytochrome c to the cytochrome c–depleted extracts restored their apoptotic activity. Cells undergoing apoptosis in vivo showed increased release of cytochrome c to their cytosol, suggesting that mitochondria may function in apoptosis by releasing cytochrome c.},
	language = {en},
	number = {1},
	urldate = {2022-04-11},
	journal = {Cell},
	author = {Liu, Xuesong and Kim, Caryn Naekyung and Yang, Jie and Jemmerson, Ronald and Wang, Xiaodong},
	month = jul,
	year = {1996},
	pages = {147--157},
}

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