@ARTICLE{Liu2016-tx,
  title    = "Single-cell analysis of long non-coding {RNAs} in the developing
              human neocortex",
  author   = "Liu, Siyuan John and Nowakowski, Tomasz J and Pollen, Alex A and
              Lui, Jan H and Horlbeck, Max A and Attenello, Frank J and He,
              Daniel and Weissman, Jonathan S and Kriegstein, Arnold R and
              Diaz, Aaron A and Lim, Daniel A",
  abstract = "BACKGROUND: Long non-coding RNAs (lncRNAs) comprise a diverse
              class of transcripts that can regulate molecular and cellular
              processes in brain development and disease. LncRNAs exhibit cell
              type- and tissue-specific expression, but little is known about
              the expression and function of lncRNAs in the developing human
              brain. Furthermore, it has been unclear whether lncRNAs are
              highly expressed in subsets of cells within tissues, despite
              appearing lowly expressed in bulk populations. RESULTS: We use
              strand-specific RNA-seq to deeply profile lncRNAs from
              polyadenylated and total RNA obtained from human neocortex at
              different stages of development, and we apply this reference to
              analyze the transcriptomes of single cells. While lncRNAs are
              generally detected at low levels in bulk tissues, single-cell
              transcriptomics of hundreds of neocortex cells reveal that many
              lncRNAs are abundantly expressed in individual cells and are cell
              type-specific. Notably, LOC646329 is a lncRNA enriched in single
              radial glia cells but is detected at low abundance in tissues.
              CRISPRi knockdown of LOC646329 indicates that this lncRNA
              regulates cell proliferation. CONCLUSION: The discrete and
              abundant expression of lncRNAs among individual cells has
              important implications for both their biological function and
              utility for distinguishing neural cell types.",
  journal  = "Genome Biol",
  volume   =  17,
  pages    = "67",
  month    =  apr,
  year     =  2016,
  keywords = "CRISPRi; Developing brain; Single-cell RNA-seq; lncRNA",
  language = "en"
}

{"_id":"aKtPzQbX6LevqDcXB","bibbaseid":"liu-nowakowski-pollen-lui-horlbeck-attenello-he-weissman-etal-singlecellanalysisoflongnoncodingrnasinthedevelopinghumanneocortex-2016","author_short":["Liu, S. J.","Nowakowski, T. J","Pollen, A. A","Lui, J. H","Horlbeck, M. A","Attenello, F. J","He, D.","Weissman, J. S","Kriegstein, A. R","Diaz, A. A","Lim, D. A"],"bibdata":{"bibtype":"article","type":"article","title":"Single-cell analysis of long non-coding RNAs in the developing human neocortex","author":[{"propositions":[],"lastnames":["Liu"],"firstnames":["Siyuan","John"],"suffixes":[]},{"propositions":[],"lastnames":["Nowakowski"],"firstnames":["Tomasz","J"],"suffixes":[]},{"propositions":[],"lastnames":["Pollen"],"firstnames":["Alex","A"],"suffixes":[]},{"propositions":[],"lastnames":["Lui"],"firstnames":["Jan","H"],"suffixes":[]},{"propositions":[],"lastnames":["Horlbeck"],"firstnames":["Max","A"],"suffixes":[]},{"propositions":[],"lastnames":["Attenello"],"firstnames":["Frank","J"],"suffixes":[]},{"propositions":[],"lastnames":["He"],"firstnames":["Daniel"],"suffixes":[]},{"propositions":[],"lastnames":["Weissman"],"firstnames":["Jonathan","S"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]},{"propositions":[],"lastnames":["Diaz"],"firstnames":["Aaron","A"],"suffixes":[]},{"propositions":[],"lastnames":["Lim"],"firstnames":["Daniel","A"],"suffixes":[]}],"abstract":"BACKGROUND: Long non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development and disease. LncRNAs exhibit cell type- and tissue-specific expression, but little is known about the expression and function of lncRNAs in the developing human brain. Furthermore, it has been unclear whether lncRNAs are highly expressed in subsets of cells within tissues, despite appearing lowly expressed in bulk populations. RESULTS: We use strand-specific RNA-seq to deeply profile lncRNAs from polyadenylated and total RNA obtained from human neocortex at different stages of development, and we apply this reference to analyze the transcriptomes of single cells. While lncRNAs are generally detected at low levels in bulk tissues, single-cell transcriptomics of hundreds of neocortex cells reveal that many lncRNAs are abundantly expressed in individual cells and are cell type-specific. Notably, LOC646329 is a lncRNA enriched in single radial glia cells but is detected at low abundance in tissues. CRISPRi knockdown of LOC646329 indicates that this lncRNA regulates cell proliferation. CONCLUSION: The discrete and abundant expression of lncRNAs among individual cells has important implications for both their biological function and utility for distinguishing neural cell types.","journal":"Genome Biol","volume":"17","pages":"67","month":"April","year":"2016","keywords":"CRISPRi; Developing brain; Single-cell RNA-seq; lncRNA","language":"en","bibtex":"@ARTICLE{Liu2016-tx,\n title = \"Single-cell analysis of long non-coding {RNAs} in the developing\n human neocortex\",\n author = \"Liu, Siyuan John and Nowakowski, Tomasz J and Pollen, Alex A and\n Lui, Jan H and Horlbeck, Max A and Attenello, Frank J and He,\n Daniel and Weissman, Jonathan S and Kriegstein, Arnold R and\n Diaz, Aaron A and Lim, Daniel A\",\n abstract = \"BACKGROUND: Long non-coding RNAs (lncRNAs) comprise a diverse\n class of transcripts that can regulate molecular and cellular\n processes in brain development and disease. LncRNAs exhibit cell\n type- and tissue-specific expression, but little is known about\n the expression and function of lncRNAs in the developing human\n brain. Furthermore, it has been unclear whether lncRNAs are\n highly expressed in subsets of cells within tissues, despite\n appearing lowly expressed in bulk populations. RESULTS: We use\n strand-specific RNA-seq to deeply profile lncRNAs from\n polyadenylated and total RNA obtained from human neocortex at\n different stages of development, and we apply this reference to\n analyze the transcriptomes of single cells. While lncRNAs are\n generally detected at low levels in bulk tissues, single-cell\n transcriptomics of hundreds of neocortex cells reveal that many\n lncRNAs are abundantly expressed in individual cells and are cell\n type-specific. Notably, LOC646329 is a lncRNA enriched in single\n radial glia cells but is detected at low abundance in tissues.\n CRISPRi knockdown of LOC646329 indicates that this lncRNA\n regulates cell proliferation. CONCLUSION: The discrete and\n abundant expression of lncRNAs among individual cells has\n important implications for both their biological function and\n utility for distinguishing neural cell types.\",\n journal = \"Genome Biol\",\n volume = 17,\n pages = \"67\",\n month = apr,\n year = 2016,\n keywords = \"CRISPRi; Developing brain; Single-cell RNA-seq; lncRNA\",\n language = \"en\"\n}\n\n","author_short":["Liu, S. J.","Nowakowski, T. J","Pollen, A. A","Lui, J. H","Horlbeck, M. A","Attenello, F. J","He, D.","Weissman, J. S","Kriegstein, A. R","Diaz, A. A","Lim, D. A"],"key":"Liu2016-tx","id":"Liu2016-tx","bibbaseid":"liu-nowakowski-pollen-lui-horlbeck-attenello-he-weissman-etal-singlecellanalysisoflongnoncodingrnasinthedevelopinghumanneocortex-2016","role":"author","urls":{},"keyword":["CRISPRi; Developing brain; Single-cell RNA-seq; lncRNA"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":["crispri; developing brain; single-cell rna-seq; lncrna"],"search_terms":["single","cell","analysis","long","non","coding","rnas","developing","human","neocortex","liu","nowakowski","pollen","lui","horlbeck","attenello","he","weissman","kriegstein","diaz","lim"],"title":"Single-cell analysis of long non-coding RNAs in the developing human neocortex","year":2016}