@article{RN115,
   author = {Liu, H. and Zhao, Y. and Yao, C. and Schmelz, E. M. and Davalos, R. V.},
   title = {Differential effects of nanosecond pulsed electric fields on cells representing progressive ovarian cancer},
   journal = {Bioelectrochemistry},
   volume = {142},
   pages = {107942},
   note = {1878-562x
Liu, Hongmei
Zhao, Yajun
Yao, Chenguo
Schmelz, Eva M
Davalos, Rafael V
Journal Article
Netherlands
2021/09/13
Bioelectrochemistry. 2021 Dec;142:107942. doi: 10.1016/j.bioelechem.2021.107942. Epub 2021 Aug 31.},
   abstract = {Nanosecond pulsed electric fields (nsPEFs) may induce differential effects on tumor cells from different disease stages and could be suitable for treating tumors by preferentially targeting the late-stage/highly aggressive tumor cells. In this study, we investigated the nsPEF responses of mouse ovarian surface epithelial (MOSE) cells representing progressive ovarian cancer from benign to malignant stages and highly aggressive tumor-initiating-like cells. We established the cell-seeded 3D collagen scaffolds cultured with or without Nocodazole (eliminating the influence of cell proliferation on ablation outcome) to observe the ablation effects at 3 h and 24 h after treatment and compared the corresponding thresholds obtained by numerically calculated electric field distribution. The results showed that nsPEFs induced larger ablation areas with lower thresholds as the cell progress from benign, malignant to a highly aggressive phenotype. This differential effect was not affected by the different doubling times of the cells, as apparent by similar ablation induction after a synergistic treatment of nsPEFs and Nocodazole. The result suggests that nsPEFs could induce preferential ablation effects on highly aggressive and malignant ovarian cancer cells than their benign counterparts. This study provides an experimental basis for the research on killing malignant tumor cells via electrical treatments and may have clinical implications for treating tumors and preventing tumor recurrence after treatment.},
   keywords = {Animals
Cell Line, Tumor
Cell Proliferation
Cell Survival
Electrochemotherapy/*methods
Female
Mice
Ovarian Neoplasms/*therapy
Cellular ablation
Electroporation
Nanosecond pulses
Ovarian cancer progression model
Selectivity},
   ISSN = {1567-5394},
   DOI = {10.1016/j.bioelechem.2021.107942},
   year = {2021},
   type = {Journal Article}
}

{"_id":"ZxxZmZjXcjHFKn6yz","bibbaseid":"liu-zhao-yao-schmelz-davalos-differentialeffectsofnanosecondpulsedelectricfieldsoncellsrepresentingprogressiveovariancancer-2021","author_short":["Liu, H.","Zhao, Y.","Yao, C.","Schmelz, E. M.","Davalos, R. V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Liu"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Zhao"],"firstnames":["Y."],"suffixes":[]},{"propositions":[],"lastnames":["Yao"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Schmelz"],"firstnames":["E.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Differential effects of nanosecond pulsed electric fields on cells representing progressive ovarian cancer","journal":"Bioelectrochemistry","volume":"142","pages":"107942","note":"1878-562x Liu, Hongmei Zhao, Yajun Yao, Chenguo Schmelz, Eva M Davalos, Rafael V Journal Article Netherlands 2021/09/13 Bioelectrochemistry. 2021 Dec;142:107942. doi: 10.1016/j.bioelechem.2021.107942. Epub 2021 Aug 31.","abstract":"Nanosecond pulsed electric fields (nsPEFs) may induce differential effects on tumor cells from different disease stages and could be suitable for treating tumors by preferentially targeting the late-stage/highly aggressive tumor cells. In this study, we investigated the nsPEF responses of mouse ovarian surface epithelial (MOSE) cells representing progressive ovarian cancer from benign to malignant stages and highly aggressive tumor-initiating-like cells. We established the cell-seeded 3D collagen scaffolds cultured with or without Nocodazole (eliminating the influence of cell proliferation on ablation outcome) to observe the ablation effects at 3 h and 24 h after treatment and compared the corresponding thresholds obtained by numerically calculated electric field distribution. The results showed that nsPEFs induced larger ablation areas with lower thresholds as the cell progress from benign, malignant to a highly aggressive phenotype. This differential effect was not affected by the different doubling times of the cells, as apparent by similar ablation induction after a synergistic treatment of nsPEFs and Nocodazole. The result suggests that nsPEFs could induce preferential ablation effects on highly aggressive and malignant ovarian cancer cells than their benign counterparts. This study provides an experimental basis for the research on killing malignant tumor cells via electrical treatments and may have clinical implications for treating tumors and preventing tumor recurrence after treatment.","keywords":"Animals Cell Line, Tumor Cell Proliferation Cell Survival Electrochemotherapy/*methods Female Mice Ovarian Neoplasms/*therapy Cellular ablation Electroporation Nanosecond pulses Ovarian cancer progression model Selectivity","issn":"1567-5394","doi":"10.1016/j.bioelechem.2021.107942","year":"2021","bibtex":"@article{RN115,\n author = {Liu, H. and Zhao, Y. and Yao, C. and Schmelz, E. M. and Davalos, R. V.},\n title = {Differential effects of nanosecond pulsed electric fields on cells representing progressive ovarian cancer},\n journal = {Bioelectrochemistry},\n volume = {142},\n pages = {107942},\n note = {1878-562x\nLiu, Hongmei\nZhao, Yajun\nYao, Chenguo\nSchmelz, Eva M\nDavalos, Rafael V\nJournal Article\nNetherlands\n2021/09/13\nBioelectrochemistry. 2021 Dec;142:107942. doi: 10.1016/j.bioelechem.2021.107942. Epub 2021 Aug 31.},\n abstract = {Nanosecond pulsed electric fields (nsPEFs) may induce differential effects on tumor cells from different disease stages and could be suitable for treating tumors by preferentially targeting the late-stage/highly aggressive tumor cells. In this study, we investigated the nsPEF responses of mouse ovarian surface epithelial (MOSE) cells representing progressive ovarian cancer from benign to malignant stages and highly aggressive tumor-initiating-like cells. We established the cell-seeded 3D collagen scaffolds cultured with or without Nocodazole (eliminating the influence of cell proliferation on ablation outcome) to observe the ablation effects at 3 h and 24 h after treatment and compared the corresponding thresholds obtained by numerically calculated electric field distribution. The results showed that nsPEFs induced larger ablation areas with lower thresholds as the cell progress from benign, malignant to a highly aggressive phenotype. This differential effect was not affected by the different doubling times of the cells, as apparent by similar ablation induction after a synergistic treatment of nsPEFs and Nocodazole. The result suggests that nsPEFs could induce preferential ablation effects on highly aggressive and malignant ovarian cancer cells than their benign counterparts. This study provides an experimental basis for the research on killing malignant tumor cells via electrical treatments and may have clinical implications for treating tumors and preventing tumor recurrence after treatment.},\n keywords = {Animals\nCell Line, Tumor\nCell Proliferation\nCell Survival\nElectrochemotherapy/*methods\nFemale\nMice\nOvarian Neoplasms/*therapy\nCellular ablation\nElectroporation\nNanosecond pulses\nOvarian cancer progression model\nSelectivity},\n ISSN = {1567-5394},\n DOI = {10.1016/j.bioelechem.2021.107942},\n year = {2021},\n type = {Journal Article}\n}\n\n","author_short":["Liu, H.","Zhao, Y.","Yao, C.","Schmelz, E. M.","Davalos, R. V."],"key":"RN115","id":"RN115","bibbaseid":"liu-zhao-yao-schmelz-davalos-differentialeffectsofnanosecondpulsedelectricfieldsoncellsrepresentingprogressiveovariancancer-2021","role":"author","urls":{},"keyword":["Animals Cell Line","Tumor Cell Proliferation Cell Survival Electrochemotherapy/*methods Female Mice Ovarian Neoplasms/*therapy Cellular ablation Electroporation Nanosecond pulses Ovarian cancer progression model Selectivity"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals cell line","tumor cell proliferation cell survival electrochemotherapy/*methods female mice ovarian neoplasms/*therapy cellular ablation electroporation nanosecond pulses ovarian cancer progression model selectivity"],"search_terms":["differential","effects","nanosecond","pulsed","electric","fields","cells","representing","progressive","ovarian","cancer","liu","zhao","yao","schmelz","davalos"],"title":"Differential effects of nanosecond pulsed electric fields on cells representing progressive ovarian cancer","year":2021}