EMAN: semiautomated software for high-resolution single-particle reconstructions. Ludtke, S., J., Baldwin, P., R., & Chiu, W. J Struct Biol, 128(1):82-97, 12, 1999. Paper Website abstract bibtex We present EMAN (Electron Micrograph ANalysis), a software package
for performing semiautomated single-particle reconstructions from
transmission electron micrographs. The goal of this project is to
provide software capable of performing single-particle reconstructions
beyond 10 A as such high-resolution data become available. A complete
single-particle reconstruction algorithm is implemented. Options
are available to generate an initial model for particles with no
symmetry, a single axis of rotational symmetry, or icosahedral symmetry.
Model refinement is an iterative process, which utilizes classification
by model-based projection matching. CTF (contrast transfer function)
parameters are determined using a new paradigm in which data from
multiple micrographs are fit simultaneously. Amplitude and phase
CTF correction is then performed automatically as part of the refinement
loop. A graphical user interface is provided, so even those with
little image processing experience will be able to begin performing
reconstructions. Advanced users can directly use the lower level
shell commands and even expand the package utilizing EMAN's extensive
image-processing library. The package was written from scratch in
C++ and is provided free of charge on our Web site. We present an
overview of the package as well as several conformance tests with
simulated data.
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title = {EMAN: semiautomated software for high-resolution single-particle reconstructions.},
type = {article},
year = {1999},
identifiers = {[object Object]},
keywords = {Algorithms; Bluetongue virus; Capsid; Cryoelectron,Computer-Assisted; Internet; Models,Molecular; Programming Languages; Protein Structu,Secondary; Software},
pages = {82-97},
volume = {128},
websites = {http://dx.doi.org/10.1006/jsbi.1999.4174},
month = {12},
institution = {Verna and Marrs McLean Department of Biochemistry, National Center for Macromolecular Imaging, Houston, Texas 77030, USA.},
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created = {2011-07-28T18:39:52.000Z},
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last_modified = {2017-03-14T11:02:08.776Z},
read = {false},
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citation_key = {Ludtke.etal:99:EMAN:},
source_type = {article},
private_publication = {false},
abstract = {We present EMAN (Electron Micrograph ANalysis), a software package
for performing semiautomated single-particle reconstructions from
transmission electron micrographs. The goal of this project is to
provide software capable of performing single-particle reconstructions
beyond 10 A as such high-resolution data become available. A complete
single-particle reconstruction algorithm is implemented. Options
are available to generate an initial model for particles with no
symmetry, a single axis of rotational symmetry, or icosahedral symmetry.
Model refinement is an iterative process, which utilizes classification
by model-based projection matching. CTF (contrast transfer function)
parameters are determined using a new paradigm in which data from
multiple micrographs are fit simultaneously. Amplitude and phase
CTF correction is then performed automatically as part of the refinement
loop. A graphical user interface is provided, so even those with
little image processing experience will be able to begin performing
reconstructions. Advanced users can directly use the lower level
shell commands and even expand the package utilizing EMAN's extensive
image-processing library. The package was written from scratch in
C++ and is provided free of charge on our Web site. We present an
overview of the package as well as several conformance tests with
simulated data.},
bibtype = {article},
author = {Ludtke, S J and Baldwin, P R and Chiu, W},
journal = {J Struct Biol},
number = {1}
}
Downloads: 0
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