Influence of shenhong Tongluo Granules in apoptosis in atherosclerosis plaques of ApoE-/- mice and its mechanisms of anti-atherosclerosis. Lyu W., Fan S., Li W., Yu J., Chen L., & Deng Y. 2016. ![Influence of shenhong Tongluo Granules in apoptosis in atherosclerosis plaques of ApoE-/- mice and its mechanisms of anti-atherosclerosis [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper abstract bibtex Objective: To explore the influence of Shenhong Tongluo Granules in the lipid levels, atherosclerosis plaques and apoptosis in the plaques of atherosclerotic apolipoprotein E (ApoE)-knokout mice, and to clarify the mechanism of Shenhong Tongluo Granules in anti-atherosclerosis. Methods: Forty SPF male mice aged six-week old were randomly divided into normal control group, model group, low dose of traditional Chinese medicine (TCM) group and high dose of TCM group (n=10) after fed adaptively for 1 week. The mice in normal control group were fed with normal diet continuously, the mice in other three groups were fed with high fat diet to establish atherosclerosis model, and then the mice in low and high doses of TCM groups were respectively given Shenhong Tongluo Granules (5.06 and 10. 12 g * kg-1 * d-1) by gavage for 6 weeks. The levels of serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) of the mice in various groups were measured after the eyeball blood was collected. The aortic arch tissue was separated, and the morphotogy and the areas of atherosclerotic plaques were observed and calculated by HE staining! the apoptosis in plaques of the mice was evaluated by TUNEL method, and the apoptosis index was calculated. The expressions of apoptosis-assoicated gene Bcl-2 and Bax protein were measured by immunohistochemical staining. Results: The serum level of TG in low dose of TCM group was lower than that in model group (P\textless0. 05); the serum levels of HDL-C in low and high doses of TCM groups were lower than that in model group (P\textless0. 05 or P\textless0. 01); the serum levels of TG and HDL-C in high dose of TCM group was higher than that in low dose of TCM group (P\textless0. 05). The areas of atherosclerotic plaques of the mice in low and high doses of TCM groups were significantly smaller than that in model group (P\textless0. 01). The apoptosis index of mice in low and high doses of TCM groups were significantly lower than that in model group (P\textless0. 01); the expression levels of Bcl-2 protein in atherosclerosis plaques of the mice in low and high doses of TCM groups were lower than that in model group, while the expression levels of Bax were significantly higher than that in model group (P\textless0. 05 or P\textless0. 01). Conclusion: Shenhong Tongluo Granules could effectively reduce the serum lipid level of ApoE-/- mice, meanwhile it could inhibit apoptosis by regulating the expressions of Bcl-2 and Bax protein, and then inhibit the progression of atherosclerotic plaques.
@misc{lyu_w._influence_2016,
title = {Influence of shenhong {Tongluo} {Granules} in apoptosis in atherosclerosis plaques of {ApoE}-/- mice and its mechanisms of anti-atherosclerosis},
url = {http://xuebao.jlu.edu.cn/yxb/EN/volumn/current.shtml},
abstract = {Objective: To explore the influence of Shenhong Tongluo Granules in the lipid levels, atherosclerosis plaques and apoptosis in the plaques of atherosclerotic apolipoprotein E (ApoE)-knokout mice, and to clarify the mechanism of Shenhong Tongluo Granules in anti-atherosclerosis. Methods: Forty SPF male mice aged six-week old were randomly divided into normal control group, model group, low dose of traditional Chinese medicine (TCM) group and high dose of TCM group (n=10) after fed adaptively for 1 week. The mice in normal control group were fed with normal diet continuously, the mice in other three groups were fed with high fat diet to establish atherosclerosis model, and then the mice in low and high doses of TCM groups were respectively given Shenhong Tongluo Granules (5.06 and 10. 12 g * kg-1 * d-1) by gavage for 6 weeks. The levels of serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) of the mice in various groups were measured after the eyeball blood was collected. The aortic arch tissue was separated, and the morphotogy and the areas of atherosclerotic plaques were observed and calculated by HE staining! the apoptosis in plaques of the mice was evaluated by TUNEL method, and the apoptosis index was calculated. The expressions of apoptosis-assoicated gene Bcl-2 and Bax protein were measured by immunohistochemical staining. Results: The serum level of TG in low dose of TCM group was lower than that in model group (P{\textless}0. 05); the serum levels of HDL-C in low and high doses of TCM groups were lower than that in model group (P{\textless}0. 05 or P{\textless}0. 01); the serum levels of TG and HDL-C in high dose of TCM group was higher than that in low dose of TCM group (P{\textless}0. 05). The areas of atherosclerotic plaques of the mice in low and high doses of TCM groups were significantly smaller than that in model group (P{\textless}0. 01). The apoptosis index of mice in low and high doses of TCM groups were significantly lower than that in model group (P{\textless}0. 01); the expression levels of Bcl-2 protein in atherosclerosis plaques of the mice in low and high doses of TCM groups were lower than that in model group, while the expression levels of Bax were significantly higher than that in model group (P{\textless}0. 05 or P{\textless}0. 01). Conclusion: Shenhong Tongluo Granules could effectively reduce the serum lipid level of ApoE-/- mice, meanwhile it could inhibit apoptosis by regulating the expressions of Bcl-2 and Bax protein, and then inhibit the progression of atherosclerotic plaques.},
journal = {Journal of Jilin University Medicine Edition},
author = {{Lyu W.} and {Fan S.} and {Li W.} and {Yu J.} and {Chen L.} and {Deng Y.}},
year = {2016},
keywords = {*Chinese medicine, *apolipoprotein E/ec [Endogenous Compound], *apoptosis, *atherosclerotic plaque, *atherosclerotic plaque/dt [Drug Therapy], *herbaceous agent/dt [Drug Therapy], *herbaceous agent/pd [Pharmacology], *herbaceous agent/po [Oral Drug Administration], *protein Bax, *protein bcl 2, *shenhong tongluo granule/dt [Drug Therapy], *shenhong tongluo granule/pd [Pharmacology], *shenhong tongluo granule/po [Oral Drug Administration], Chinese medicine, TUNEL assay, animal experiment, animal model, animal tissue, aorta arch, article, atherosclerotic plaque/dt [Drug Therapy], cholesterol blood level, cholesterol/ec [Endogenous Compound], control group, controlled study, disease course, disease model, drug mechanism, drug megadose, endogenous compound, enteric feeding, eosin, eyeball, gene expression, hematoxylin, high density lipoprotein cholesterol, high density lipoprotein cholesterol/ec [Endogenous Compound], immunohistochemistry, lipid diet, low density lipoprotein cholesterol, low density lipoprotein cholesterol/ec [Endogenous Compound], low drug dose, male, mouse, mouse model, nonhuman, preschool child, protein Bax/ec [Endogenous Compound], protein bcl 2/ec [Endogenous Compound], protein expression, staining, triacylglycerol, triacylglycerol/ec [Endogenous Compound], unclassified drug}
}
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{"_id":"6EYsJjJ4CgzHukkm5","bibbaseid":"lyuw-fans-liw-yuj-chenl-dengy-influenceofshenhongtongluogranulesinapoptosisinatherosclerosisplaquesofapoemiceanditsmechanismsofantiatherosclerosis-2016","downloads":0,"creationDate":"2017-05-12T20:20:57.489Z","title":"Influence of shenhong Tongluo Granules in apoptosis in atherosclerosis plaques of ApoE-/- mice and its mechanisms of anti-atherosclerosis","author_short":["Lyu W.","Fan S.","Li W.","Yu J.","Chen L.","Deng Y."],"year":2016,"bibtype":"misc","biburl":"http://bibbase.org/zotero/gxu917","bibdata":{"bibtype":"misc","type":"misc","title":"Influence of shenhong Tongluo Granules in apoptosis in atherosclerosis plaques of ApoE-/- mice and its mechanisms of anti-atherosclerosis","url":"http://xuebao.jlu.edu.cn/yxb/EN/volumn/current.shtml","abstract":"Objective: To explore the influence of Shenhong Tongluo Granules in the lipid levels, atherosclerosis plaques and apoptosis in the plaques of atherosclerotic apolipoprotein E (ApoE)-knokout mice, and to clarify the mechanism of Shenhong Tongluo Granules in anti-atherosclerosis. Methods: Forty SPF male mice aged six-week old were randomly divided into normal control group, model group, low dose of traditional Chinese medicine (TCM) group and high dose of TCM group (n=10) after fed adaptively for 1 week. The mice in normal control group were fed with normal diet continuously, the mice in other three groups were fed with high fat diet to establish atherosclerosis model, and then the mice in low and high doses of TCM groups were respectively given Shenhong Tongluo Granules (5.06 and 10. 12 g * kg-1 * d-1) by gavage for 6 weeks. The levels of serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) of the mice in various groups were measured after the eyeball blood was collected. The aortic arch tissue was separated, and the morphotogy and the areas of atherosclerotic plaques were observed and calculated by HE staining! the apoptosis in plaques of the mice was evaluated by TUNEL method, and the apoptosis index was calculated. The expressions of apoptosis-assoicated gene Bcl-2 and Bax protein were measured by immunohistochemical staining. Results: The serum level of TG in low dose of TCM group was lower than that in model group (P\\textless0. 05); the serum levels of HDL-C in low and high doses of TCM groups were lower than that in model group (P\\textless0. 05 or P\\textless0. 01); the serum levels of TG and HDL-C in high dose of TCM group was higher than that in low dose of TCM group (P\\textless0. 05). The areas of atherosclerotic plaques of the mice in low and high doses of TCM groups were significantly smaller than that in model group (P\\textless0. 01). The apoptosis index of mice in low and high doses of TCM groups were significantly lower than that in model group (P\\textless0. 01); the expression levels of Bcl-2 protein in atherosclerosis plaques of the mice in low and high doses of TCM groups were lower than that in model group, while the expression levels of Bax were significantly higher than that in model group (P\\textless0. 05 or P\\textless0. 01). Conclusion: Shenhong Tongluo Granules could effectively reduce the serum lipid level of ApoE-/- mice, meanwhile it could inhibit apoptosis by regulating the expressions of Bcl-2 and Bax protein, and then inhibit the progression of atherosclerotic plaques.","journal":"Journal of Jilin University Medicine Edition","author":[{"firstnames":[],"propositions":[],"lastnames":["Lyu W."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Fan S."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Li W."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Yu J."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Chen L."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Deng Y."],"suffixes":[]}],"year":"2016","keywords":"*Chinese medicine, *apolipoprotein E/ec [Endogenous Compound], *apoptosis, *atherosclerotic plaque, *atherosclerotic plaque/dt [Drug Therapy], *herbaceous agent/dt [Drug Therapy], *herbaceous agent/pd [Pharmacology], *herbaceous agent/po [Oral Drug Administration], *protein Bax, *protein bcl 2, *shenhong tongluo granule/dt [Drug Therapy], *shenhong tongluo granule/pd [Pharmacology], *shenhong tongluo granule/po [Oral Drug Administration], Chinese medicine, TUNEL assay, animal experiment, animal model, animal tissue, aorta arch, article, atherosclerotic plaque/dt [Drug Therapy], cholesterol blood level, cholesterol/ec [Endogenous Compound], control group, controlled study, disease course, disease model, drug mechanism, drug megadose, endogenous compound, enteric feeding, eosin, eyeball, gene expression, hematoxylin, high density lipoprotein cholesterol, high density lipoprotein cholesterol/ec [Endogenous Compound], immunohistochemistry, lipid diet, low density lipoprotein cholesterol, low density lipoprotein cholesterol/ec [Endogenous Compound], low drug dose, male, mouse, mouse model, nonhuman, preschool child, protein Bax/ec [Endogenous Compound], protein bcl 2/ec [Endogenous Compound], protein expression, staining, triacylglycerol, triacylglycerol/ec [Endogenous Compound], unclassified drug","bibtex":"@misc{lyu_w._influence_2016,\n\ttitle = {Influence of shenhong {Tongluo} {Granules} in apoptosis in atherosclerosis plaques of {ApoE}-/- mice and its mechanisms of anti-atherosclerosis},\n\turl = {http://xuebao.jlu.edu.cn/yxb/EN/volumn/current.shtml},\n\tabstract = {Objective: To explore the influence of Shenhong Tongluo Granules in the lipid levels, atherosclerosis plaques and apoptosis in the plaques of atherosclerotic apolipoprotein E (ApoE)-knokout mice, and to clarify the mechanism of Shenhong Tongluo Granules in anti-atherosclerosis. Methods: Forty SPF male mice aged six-week old were randomly divided into normal control group, model group, low dose of traditional Chinese medicine (TCM) group and high dose of TCM group (n=10) after fed adaptively for 1 week. The mice in normal control group were fed with normal diet continuously, the mice in other three groups were fed with high fat diet to establish atherosclerosis model, and then the mice in low and high doses of TCM groups were respectively given Shenhong Tongluo Granules (5.06 and 10. 12 g * kg-1 * d-1) by gavage for 6 weeks. The levels of serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) of the mice in various groups were measured after the eyeball blood was collected. The aortic arch tissue was separated, and the morphotogy and the areas of atherosclerotic plaques were observed and calculated by HE staining! the apoptosis in plaques of the mice was evaluated by TUNEL method, and the apoptosis index was calculated. The expressions of apoptosis-assoicated gene Bcl-2 and Bax protein were measured by immunohistochemical staining. Results: The serum level of TG in low dose of TCM group was lower than that in model group (P{\\textless}0. 05); the serum levels of HDL-C in low and high doses of TCM groups were lower than that in model group (P{\\textless}0. 05 or P{\\textless}0. 01); the serum levels of TG and HDL-C in high dose of TCM group was higher than that in low dose of TCM group (P{\\textless}0. 05). The areas of atherosclerotic plaques of the mice in low and high doses of TCM groups were significantly smaller than that in model group (P{\\textless}0. 01). The apoptosis index of mice in low and high doses of TCM groups were significantly lower than that in model group (P{\\textless}0. 01); the expression levels of Bcl-2 protein in atherosclerosis plaques of the mice in low and high doses of TCM groups were lower than that in model group, while the expression levels of Bax were significantly higher than that in model group (P{\\textless}0. 05 or P{\\textless}0. 01). Conclusion: Shenhong Tongluo Granules could effectively reduce the serum lipid level of ApoE-/- mice, meanwhile it could inhibit apoptosis by regulating the expressions of Bcl-2 and Bax protein, and then inhibit the progression of atherosclerotic plaques.},\n\tjournal = {Journal of Jilin University Medicine Edition},\n\tauthor = {{Lyu W.} and {Fan S.} and {Li W.} and {Yu J.} and {Chen L.} and {Deng Y.}},\n\tyear = {2016},\n\tkeywords = {*Chinese medicine, *apolipoprotein E/ec [Endogenous Compound], *apoptosis, *atherosclerotic plaque, *atherosclerotic plaque/dt [Drug Therapy], *herbaceous agent/dt [Drug Therapy], *herbaceous agent/pd [Pharmacology], *herbaceous agent/po [Oral Drug Administration], *protein Bax, *protein bcl 2, *shenhong tongluo granule/dt [Drug Therapy], *shenhong tongluo granule/pd [Pharmacology], *shenhong tongluo granule/po [Oral Drug Administration], Chinese medicine, TUNEL assay, animal experiment, animal model, animal tissue, aorta arch, article, atherosclerotic 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