De novo sequencing and comprehensive analysis of the mutant transcriptome from purple sweet potato (Ipomoea batatas L.). Ma, P.; Bian, X.; Jia, Z.; Guo, X.; and Xie, Y. Gene, September, 2015.
De novo sequencing and comprehensive analysis of the mutant transcriptome from purple sweet potato (Ipomoea batatas L.) [link]Paper  doi  abstract   bibtex   
Purple sweet potatoes, rich in anthocyanin, have been widely favored in light of increasing awareness of health and food safety. In this study, a mutant of purple sweet potato (white peel and flesh) was used to study anthocyanin metabolism by high-throughput RNA sequencing and comparative analysis of the mutant and wild type transcriptomes. A total of 88,509 unigenes ranging from 200 nt to 14,986 nt with an average length of 849 nt were obtained. Unigenes were assigned to Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Functional enrichment using GO and KEGG annotations showed that 3828 of the differently expressed genes probably influenced many important biological and metabolic pathways, including anthocyanin biosynthesis. Most importantly, the structural and transcription factor genes that contribute to anthocyanin biosynthesis were downregulated in the mutant. The unigene dataset that was used to discover the anthocyanin candidate genes can serve as a comprehensive resource for molecular research in sweet potato.
@article{ma_novo_2015,
	title = {De novo sequencing and comprehensive analysis of the mutant transcriptome from purple sweet potato ({Ipomoea} batatas {L}.)},
	issn = {03781119},
	url = {http://linkinghub.elsevier.com/retrieve/pii/S0378111915011658},
	doi = {10.1016/j.gene.2015.09.056},
	abstract = {Purple sweet potatoes, rich in anthocyanin, have been widely favored in light of increasing awareness of health and food safety. In this study, a mutant of purple sweet potato (white peel and flesh) was used to study anthocyanin metabolism by high-throughput RNA sequencing and comparative analysis of the mutant and wild type transcriptomes. A total of 88,509 unigenes ranging from 200 nt to 14,986 nt with an average length of 849 nt were obtained. Unigenes were assigned to Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Functional enrichment using GO and KEGG annotations showed that 3828 of the differently expressed genes probably influenced many important biological and metabolic pathways, including anthocyanin biosynthesis. Most importantly, the structural and transcription factor genes that contribute to anthocyanin biosynthesis were downregulated in the mutant. The unigene dataset that was used to discover the anthocyanin candidate genes can serve as a comprehensive resource for molecular research in sweet potato.},
	language = {en},
	urldate = {2015-10-01},
	journal = {Gene},
	author = {Ma, Peiyong and Bian, Xiaofeng and Jia, Zhaodong and Guo, Xiaoding and Xie, Yizhi},
	month = sep,
	year = {2015},
	keywords = {Anthocyanin, Gene expression, Mutant, sweet potato, transcriptome},
}
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