RACK1A positively regulates opening of the apical hook in Arabidopsis thaliana via suppression of its auxin response gradient. Ma, Q., Liu, S., Doyle, S. M., Raggi, S., Pařízková, B., Barange, D. K., Ratnakaram, H., Wilkinson, E. G., Crespo Garcia, I., Bygdell, J., Wingsle, G., Boer, D. R., Strader, L. C., Almqvist, F., Novák, O., & Robert, S. Proceedings of the National Academy of Sciences, 122(30):e2407224122, July, 2025. Publisher: Proceedings of the National Academy of Sciences![RACK1A positively regulates opening of the apical hook in Arabidopsis thaliana via suppression of its auxin response gradient [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Apical hook development is an ideal model for studying differential growth in plants and is controlled by complex phytohormonal crosstalk, with auxin being the major player. Here, we identified a bioactive small molecule that decelerates apical hook opening in Arabidopsis thaliana. Our genetic studies suggest that this molecule enhances or maintains the auxin maximum found in the inner hook side and requires certain auxin signaling components to modulate apical hook opening. Using biochemical approaches, we then revealed the WD40 repeat scaffold protein RECEPTOR FOR ACTIVATED C KINASE 1A (RACK1A) as a direct target of this compound. We present data in support of RACK1A playing a positive role in apical hook opening by activating specific auxin signaling mechanisms and negatively regulating the differential auxin response gradient across the hook, thereby adjusting differential cell growth, an essential process for organ structure and function in plants.
@article{ma_rack1a_2025,
title = {{RACK1A} positively regulates opening of the apical hook in {Arabidopsis} thaliana via suppression of its auxin response gradient},
volume = {122},
url = {https://www.pnas.org/doi/10.1073/pnas.2407224122},
doi = {10.1073/pnas.2407224122},
abstract = {Apical hook development is an ideal model for studying differential growth in plants and is controlled by complex phytohormonal crosstalk, with auxin being the major player. Here, we identified a bioactive small molecule that decelerates apical hook opening in Arabidopsis thaliana. Our genetic studies suggest that this molecule enhances or maintains the auxin maximum found in the inner hook side and requires certain auxin signaling components to modulate apical hook opening. Using biochemical approaches, we then revealed the WD40 repeat scaffold protein RECEPTOR FOR ACTIVATED C KINASE 1A (RACK1A) as a direct target of this compound. We present data in support of RACK1A playing a positive role in apical hook opening by activating specific auxin signaling mechanisms and negatively regulating the differential auxin response gradient across the hook, thereby adjusting differential cell growth, an essential process for organ structure and function in plants.},
number = {30},
urldate = {2025-07-25},
journal = {Proceedings of the National Academy of Sciences},
author = {Ma, Qian and Liu, Sijia and Doyle, Siamsa M. and Raggi, Sara and Pařízková, Barbora and Barange, Deepak Kumar and Ratnakaram, Hemamshu and Wilkinson, Edward G. and Crespo Garcia, Isidro and Bygdell, Joakim and Wingsle, Gunnar and Boer, Dirk Roeland and Strader, Lucia C. and Almqvist, Fredrik and Novák, Ondřej and Robert, Stéphanie},
month = jul,
year = {2025},
note = {Publisher: Proceedings of the National Academy of Sciences},
pages = {e2407224122},
}
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