Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture. Mahboubi, A., Delhomme, N., Häggström, S., & Hanson, J. Plant Methods, 17(1):92, August, 2021.
Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture [link]Paper  doi  abstract   bibtex   
Translation is a tightly regulated process, controlling the rate of protein synthesis in cells. Ribosome sequencing (Ribo-Seq) is a recently developed tool for studying actively translated mRNA and can thus directly address translational regulation. Ribo-Seq libraries need to be sequenced to a great depth due to high contamination by rRNA and other contaminating nucleic acid fragments. Deep sequencing is expensive, and it generates large volumes of data, making data analysis complicated and time consuming.
@article{mahboubi_small-scale_2021,
	title = {Small-scale sequencing enables quality assessment of {Ribo}-{Seq} data: an example from {Arabidopsis} cell culture},
	volume = {17},
	issn = {1746-4811},
	shorttitle = {Small-scale sequencing enables quality assessment of {Ribo}-{Seq} data},
	url = {https://doi.org/10.1186/s13007-021-00791-w},
	doi = {10.1186/s13007-021-00791-w},
	abstract = {Translation is a tightly regulated process, controlling the rate of protein synthesis in cells. Ribosome sequencing (Ribo-Seq) is a recently developed tool for studying actively translated mRNA and can thus directly address translational regulation. Ribo-Seq libraries need to be sequenced to a great depth due to high contamination by rRNA and other contaminating nucleic acid fragments. Deep sequencing is expensive, and it generates large volumes of data, making data analysis complicated and time consuming.},
	number = {1},
	urldate = {2021-10-14},
	journal = {Plant Methods},
	author = {Mahboubi, Amir and Delhomme, Nicolas and Häggström, Sara and Hanson, Johannes},
	month = aug,
	year = {2021},
	keywords = {Evaluation of sequencing library quality, Ribo-Seq, Ribosomal profiling, Translation, Translational profiling},
	pages = {92},
}

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