The ability of a bacteriocin of Streptococcus bovis HC5 (bovicin HC5) to inhibit Clostridium aminophilum, an obligate amino acid fermenting bacterium from the rumen. Mantovani, H. & Russell, J. Anaerobe, 2002. abstract bibtex Ruminant nutritionists have used antibiotics to inhibit Gram-positive bacteria and alter the end products of ruminal fermentation, but this practice has been criticized. Many Gram-positive ruminal bacteria can be inhibited by bacteriocins, but bacteriocin resistance can circumvent this inhibition. C. aminophilum is a detrimental ruminal bacterium that produces large amounts of ammonia, and it becomes highly resistant to the antibiotic, monensin, and at least one naturally occurring bacteriocin (butyrivibriocin JL5). C. aminophilum, was inhibited by Streptococcus bovis HC5, a bacterium that produces bovicin HC5, but not S. bovis JB1, a non-bacteriocin producing strain. Co-culture experiments indicated that the inhibition persisted for at least six transfers. When C. aminophilum F was inoculated (5% v/v) into culture broth that had increasing amounts of semi-purified bovicin HC5, growth and ammonia production were only observed if the concentration was less than 12.5 activity units (AU)/mL. C. aminophilum F cultures treated with 10 AU/mL of bovicin HC5, grew, but the growth rate was noticeably slower, and there was a pronounced lag before growth and ammonia production were observed. Cultures that were transferred successively (7 times) with 10 AU/mL bovicin HC5 tolerated 4-fold more bovicin HC5 than untreated controls, but growth was never observed if bovicin HC5 was ≥50 AU/mL. Bovicin HC5 caused an efflux of potassium from C. aminophilum F, but the efflux rate was at least 8 times slower than S. bovis JB1. If the pH was decreased from 6.5 to 5.5 with HCl, the efflux rate was at least 6-fold faster. Given the fact that the European Union has already proposed a ban on feed antibiotics, bovicin HC5 could be an alternative.
@article{
title = {The ability of a bacteriocin of Streptococcus bovis HC5 (bovicin HC5) to inhibit Clostridium aminophilum, an obligate amino acid fermenting bacterium from the rumen},
type = {article},
year = {2002},
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keywords = {Ammonia,Bacteriocin,Clostridium aminophilum,Rumen,Streptococcus bovis},
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abstract = {Ruminant nutritionists have used antibiotics to inhibit Gram-positive bacteria and alter the end products of ruminal fermentation, but this practice has been criticized. Many Gram-positive ruminal bacteria can be inhibited by bacteriocins, but bacteriocin resistance can circumvent this inhibition. C. aminophilum is a detrimental ruminal bacterium that produces large amounts of ammonia, and it becomes highly resistant to the antibiotic, monensin, and at least one naturally occurring bacteriocin (butyrivibriocin JL5). C. aminophilum, was inhibited by Streptococcus bovis HC5, a bacterium that produces bovicin HC5, but not S. bovis JB1, a non-bacteriocin producing strain. Co-culture experiments indicated that the inhibition persisted for at least six transfers. When C. aminophilum F was inoculated (5% v/v) into culture broth that had increasing amounts of semi-purified bovicin HC5, growth and ammonia production were only observed if the concentration was less than 12.5 activity units (AU)/mL. C. aminophilum F cultures treated with 10 AU/mL of bovicin HC5, grew, but the growth rate was noticeably slower, and there was a pronounced lag before growth and ammonia production were observed. Cultures that were transferred successively (7 times) with 10 AU/mL bovicin HC5 tolerated 4-fold more bovicin HC5 than untreated controls, but growth was never observed if bovicin HC5 was ≥50 AU/mL. Bovicin HC5 caused an efflux of potassium from C. aminophilum F, but the efflux rate was at least 8 times slower than S. bovis JB1. If the pH was decreased from 6.5 to 5.5 with HCl, the efflux rate was at least 6-fold faster. Given the fact that the European Union has already proposed a ban on feed antibiotics, bovicin HC5 could be an alternative.},
bibtype = {article},
author = {Mantovani, H.C. and Russell, J.B.},
journal = {Anaerobe},
number = {5}
}
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C. aminophilum F cultures treated with 10 AU/mL of bovicin HC5, grew, but the growth rate was noticeably slower, and there was a pronounced lag before growth and ammonia production were observed. Cultures that were transferred successively (7 times) with 10 AU/mL bovicin HC5 tolerated 4-fold more bovicin HC5 than untreated controls, but growth was never observed if bovicin HC5 was ≥50 AU/mL. Bovicin HC5 caused an efflux of potassium from C. aminophilum F, but the efflux rate was at least 8 times slower than S. bovis JB1. If the pH was decreased from 6.5 to 5.5 with HCl, the efflux rate was at least 6-fold faster. 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C. aminophilum F cultures treated with 10 AU/mL of bovicin HC5, grew, but the growth rate was noticeably slower, and there was a pronounced lag before growth and ammonia production were observed. Cultures that were transferred successively (7 times) with 10 AU/mL bovicin HC5 tolerated 4-fold more bovicin HC5 than untreated controls, but growth was never observed if bovicin HC5 was ≥50 AU/mL. Bovicin HC5 caused an efflux of potassium from C. aminophilum F, but the efflux rate was at least 8 times slower than S. bovis JB1. If the pH was decreased from 6.5 to 5.5 with HCl, the efflux rate was at least 6-fold faster. 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