Photosynthetic Efficiency and Antioxidant Defense Potential are Key Players in Inducing Drought Tolerance in Transgenic Tobacco Plants Over-Expressing AVP1. Manzoor, H., Anjam, M. S., Saeed, F., Rasul, S., Yousaf, S., Kirn, A., Qureshi, M. K., Zafar, Z. U., Ashraf, M., & Athar, H. Journal of Plant Growth Regulation, 41(7):2653–2668, October, 2022. ![Photosynthetic Efficiency and Antioxidant Defense Potential are Key Players in Inducing Drought Tolerance in Transgenic Tobacco Plants Over-Expressing AVP1 [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Plants have developed a number of physiological, biochemical, and molecular strategies to overcome water deficit conditions. Arabidopsis vacuolar pyrophosphatase 1 (AVP1) can enhance stress tolerance in plants through ion homeostasis, redox balance, and photosynthetic activity. In the present study, three transgenic tobacco lines overexpressing AVP1 gene were compared with a wild type (WT) line to assess the role of AVP1 in inducing drought tolerance. For this purpose, four-week old tobacco plants were subjected to cyclic drought for three weeks and were evaluated for different physio-biochemical parameters. Drought stress reduced shoot and root dry mass (85% and 73%), relative water content (55%), K+ accumulation (42%), and Photosystem-II (PSII) efficiency (27%), but this effect was less on transgenic tobacco lines of AVP1 (L2, L3). Drought stress decreased performance index (PIABS) by 48% in WT plants, which is linked with substantial increase in specific energy fluxes through PSII per active reaction center (ABS/RC, TR0/RC) and cause a decrease in electron transport (ET0/RC) by 47%, that resulted in damage of donor end of PSII. However, AVP1 transgenic line L3 showed minimum decrease in quantum efficiency of PSII (3%) and PIABS (12%) due to lesser drought-induced oxidative damage and having higher catalase activity (96%). As compared to control plants, drought-stressed WT plants significantly enhanced hydrogen peroxide and malondialdehyde production by 101% and 98%, respectively. However, transgenic lines particularly L2 and L3 had lower levels of H2O2 (26% and 14%) and MDA (56% and 16%), which were positively associated with higher activities of catalase. In conclusion, AVP1 gene helped to maintain water and K+ homeostasis, which in turn affected the redox balance and PSII structural and functional stability.
@article{manzoor_photosynthetic_2022,
title = {Photosynthetic {Efficiency} and {Antioxidant} {Defense} {Potential} are {Key} {Players} in {Inducing} {Drought} {Tolerance} in {Transgenic} {Tobacco} {Plants} {Over}-{Expressing} {AVP1}},
volume = {41},
issn = {1435-8107},
url = {https://doi.org/10.1007/s00344-021-10464-6},
doi = {10.1007/s00344-021-10464-6},
abstract = {Plants have developed a number of physiological, biochemical, and molecular strategies to overcome water deficit conditions. Arabidopsis vacuolar pyrophosphatase 1 (AVP1) can enhance stress tolerance in plants through ion homeostasis, redox balance, and photosynthetic activity. In the present study, three transgenic tobacco lines overexpressing AVP1 gene were compared with a wild type (WT) line to assess the role of AVP1 in inducing drought tolerance. For this purpose, four-week old tobacco plants were subjected to cyclic drought for three weeks and were evaluated for different physio-biochemical parameters. Drought stress reduced shoot and root dry mass (85\% and 73\%), relative water content (55\%), K+ accumulation (42\%), and Photosystem-II (PSII) efficiency (27\%), but this effect was less on transgenic tobacco lines of AVP1 (L2, L3). Drought stress decreased performance index (PIABS) by 48\% in WT plants, which is linked with substantial increase in specific energy fluxes through PSII per active reaction center (ABS/RC, TR0/RC) and cause a decrease in electron transport (ET0/RC) by 47\%, that resulted in damage of donor end of PSII. However, AVP1 transgenic line L3 showed minimum decrease in quantum efficiency of PSII (3\%) and PIABS (12\%) due to lesser drought-induced oxidative damage and having higher catalase activity (96\%). As compared to control plants, drought-stressed WT plants significantly enhanced hydrogen peroxide and malondialdehyde production by 101\% and 98\%, respectively. However, transgenic lines particularly L2 and L3 had lower levels of H2O2 (26\% and 14\%) and MDA (56\% and 16\%), which were positively associated with higher activities of catalase. In conclusion, AVP1 gene helped to maintain water and K+ homeostasis, which in turn affected the redox balance and PSII structural and functional stability.},
language = {en},
number = {7},
urldate = {2024-08-30},
journal = {Journal of Plant Growth Regulation},
author = {Manzoor, Hamid and Anjam, Muhammad Shahzad and Saeed, Fozia and Rasul, Sumaira and Yousaf, Saira and Kirn, Ayesha and Qureshi, Muhammad Kamran and Zafar, Zafar Ullah and Ashraf, Muhammad and Athar, Habib-ur-Rehman},
month = oct,
year = {2022},
keywords = {Arabidopsis vacuolar pyrophosphatase, Catalase, Drought, JIP-test, Photosystem-II, Reactive oxygen species},
pages = {2653--2668},
}
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Arabidopsis vacuolar pyrophosphatase 1 (AVP1) can enhance stress tolerance in plants through ion homeostasis, redox balance, and photosynthetic activity. In the present study, three transgenic tobacco lines overexpressing AVP1 gene were compared with a wild type (WT) line to assess the role of AVP1 in inducing drought tolerance. For this purpose, four-week old tobacco plants were subjected to cyclic drought for three weeks and were evaluated for different physio-biochemical parameters. Drought stress reduced shoot and root dry mass (85% and 73%), relative water content (55%), K+ accumulation (42%), and Photosystem-II (PSII) efficiency (27%), but this effect was less on transgenic tobacco lines of AVP1 (L2, L3). Drought stress decreased performance index (PIABS) by 48% in WT plants, which is linked with substantial increase in specific energy fluxes through PSII per active reaction center (ABS/RC, TR0/RC) and cause a decrease in electron transport (ET0/RC) by 47%, that resulted in damage of donor end of PSII. However, AVP1 transgenic line L3 showed minimum decrease in quantum efficiency of PSII (3%) and PIABS (12%) due to lesser drought-induced oxidative damage and having higher catalase activity (96%). As compared to control plants, drought-stressed WT plants significantly enhanced hydrogen peroxide and malondialdehyde production by 101% and 98%, respectively. However, transgenic lines particularly L2 and L3 had lower levels of H2O2 (26% and 14%) and MDA (56% and 16%), which were positively associated with higher activities of catalase. In conclusion, AVP1 gene helped to maintain water and K+ homeostasis, which in turn affected the redox balance and PSII structural and functional stability.","language":"en","number":"7","urldate":"2024-08-30","journal":"Journal of Plant Growth Regulation","author":[{"propositions":[],"lastnames":["Manzoor"],"firstnames":["Hamid"],"suffixes":[]},{"propositions":[],"lastnames":["Anjam"],"firstnames":["Muhammad","Shahzad"],"suffixes":[]},{"propositions":[],"lastnames":["Saeed"],"firstnames":["Fozia"],"suffixes":[]},{"propositions":[],"lastnames":["Rasul"],"firstnames":["Sumaira"],"suffixes":[]},{"propositions":[],"lastnames":["Yousaf"],"firstnames":["Saira"],"suffixes":[]},{"propositions":[],"lastnames":["Kirn"],"firstnames":["Ayesha"],"suffixes":[]},{"propositions":[],"lastnames":["Qureshi"],"firstnames":["Muhammad","Kamran"],"suffixes":[]},{"propositions":[],"lastnames":["Zafar"],"firstnames":["Zafar","Ullah"],"suffixes":[]},{"propositions":[],"lastnames":["Ashraf"],"firstnames":["Muhammad"],"suffixes":[]},{"propositions":[],"lastnames":["Athar"],"firstnames":["Habib-ur-Rehman"],"suffixes":[]}],"month":"October","year":"2022","keywords":"Arabidopsis vacuolar pyrophosphatase, Catalase, Drought, JIP-test, Photosystem-II, Reactive oxygen species","pages":"2653–2668","bibtex":"@article{manzoor_photosynthetic_2022,\n\ttitle = {Photosynthetic {Efficiency} and {Antioxidant} {Defense} {Potential} are {Key} {Players} in {Inducing} {Drought} {Tolerance} in {Transgenic} {Tobacco} {Plants} {Over}-{Expressing} {AVP1}},\n\tvolume = {41},\n\tissn = {1435-8107},\n\turl = {https://doi.org/10.1007/s00344-021-10464-6},\n\tdoi = {10.1007/s00344-021-10464-6},\n\tabstract = {Plants have developed a number of physiological, biochemical, and molecular strategies to overcome water deficit conditions. Arabidopsis vacuolar pyrophosphatase 1 (AVP1) can enhance stress tolerance in plants through ion homeostasis, redox balance, and photosynthetic activity. In the present study, three transgenic tobacco lines overexpressing AVP1 gene were compared with a wild type (WT) line to assess the role of AVP1 in inducing drought tolerance. For this purpose, four-week old tobacco plants were subjected to cyclic drought for three weeks and were evaluated for different physio-biochemical parameters. Drought stress reduced shoot and root dry mass (85\\% and 73\\%), relative water content (55\\%), K+ accumulation (42\\%), and Photosystem-II (PSII) efficiency (27\\%), but this effect was less on transgenic tobacco lines of AVP1 (L2, L3). Drought stress decreased performance index (PIABS) by 48\\% in WT plants, which is linked with substantial increase in specific energy fluxes through PSII per active reaction center (ABS/RC, TR0/RC) and cause a decrease in electron transport (ET0/RC) by 47\\%, that resulted in damage of donor end of PSII. However, AVP1 transgenic line L3 showed minimum decrease in quantum efficiency of PSII (3\\%) and PIABS (12\\%) due to lesser drought-induced oxidative damage and having higher catalase activity (96\\%). As compared to control plants, drought-stressed WT plants significantly enhanced hydrogen peroxide and malondialdehyde production by 101\\% and 98\\%, respectively. However, transgenic lines particularly L2 and L3 had lower levels of H2O2 (26\\% and 14\\%) and MDA (56\\% and 16\\%), which were positively associated with higher activities of catalase. 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