Dynamic structure of DNA complexes. Fluorometric measurement of hydrogen-deuterium exchange kinetics of DNA-bound ethidium dimer and acridine-ethidium dimer. Markovits, J., Ramstein, J., Roques, B. P., & Pecq, J. B. L. Biochemistry, 22(13):3231–3237, June, 1983. tex.ids= markovitsDynamicStructureDNA1983Paper doi abstract bibtex The hydrogen-deuterium (H-D) exchange kinetics of free and DNA-bound ethidium dimer and acridine-ethidium heterodimer were measured by stopped flow using fluorescence detection. This technique allowed a very accurate measurement of the exchange process. The H-D exchange kinetics were measured in various environments. In some cases, it was observed that the H-D exchange was much faster than the dissociation rate of dimer-DNA complexes. This showed that the exchange was taking place directly from the bound state. Furthermore, the action of a catalyst (imidazolium ion) on the rate of H-D exchange showed that a dynamic structural fluctuation of the ligand in its DNA complex was a necessary step on the exchange process.
@article{Markovits1983,
title = {Dynamic structure of {DNA} complexes. {Fluorometric} measurement of hydrogen-deuterium exchange kinetics of {DNA}-bound ethidium dimer and acridine-ethidium dimer},
volume = {22},
issn = {0006-2960},
url = {http://pubs.acs.org/doi/abs/10.1021/bi00282a030},
doi = {10.1021/bi00282a030},
abstract = {The hydrogen-deuterium (H-D) exchange kinetics of free and DNA-bound ethidium dimer and acridine-ethidium heterodimer were measured by stopped flow using fluorescence detection. This technique allowed a very accurate measurement of the exchange process. The H-D exchange kinetics were measured in various environments. In some cases, it was observed that the H-D exchange was much faster than the dissociation rate of dimer-DNA complexes. This showed that the exchange was taking place directly from the bound state. Furthermore, the action of a catalyst (imidazolium ion) on the rate of H-D exchange showed that a dynamic structural fluctuation of the ligand in its DNA complex was a necessary step on the exchange process.},
number = {13},
journal = {Biochemistry},
author = {Markovits, J. and Ramstein, J. and Roques, B. P. and Pecq, J. B. Le},
month = jun,
year = {1983},
pmid = {6882747},
note = {tex.ids= markovitsDynamicStructureDNA1983},
pages = {3231--3237},
}
Downloads: 0
{"_id":"Tc4jbJwebtxJWXCFR","bibbaseid":"markovits-ramstein-roques-pecq-dynamicstructureofdnacomplexesfluorometricmeasurementofhydrogendeuteriumexchangekineticsofdnaboundethidiumdimerandacridineethidiumdimer-1983","author_short":["Markovits, J.","Ramstein, J.","Roques, B. P.","Pecq, J. B. L."],"bibdata":{"bibtype":"article","type":"article","title":"Dynamic structure of DNA complexes. Fluorometric measurement of hydrogen-deuterium exchange kinetics of DNA-bound ethidium dimer and acridine-ethidium dimer","volume":"22","issn":"0006-2960","url":"http://pubs.acs.org/doi/abs/10.1021/bi00282a030","doi":"10.1021/bi00282a030","abstract":"The hydrogen-deuterium (H-D) exchange kinetics of free and DNA-bound ethidium dimer and acridine-ethidium heterodimer were measured by stopped flow using fluorescence detection. This technique allowed a very accurate measurement of the exchange process. The H-D exchange kinetics were measured in various environments. In some cases, it was observed that the H-D exchange was much faster than the dissociation rate of dimer-DNA complexes. This showed that the exchange was taking place directly from the bound state. Furthermore, the action of a catalyst (imidazolium ion) on the rate of H-D exchange showed that a dynamic structural fluctuation of the ligand in its DNA complex was a necessary step on the exchange process.","number":"13","journal":"Biochemistry","author":[{"propositions":[],"lastnames":["Markovits"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Ramstein"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Roques"],"firstnames":["B.","P."],"suffixes":[]},{"propositions":[],"lastnames":["Pecq"],"firstnames":["J.","B.","Le"],"suffixes":[]}],"month":"June","year":"1983","pmid":"6882747","note":"tex.ids= markovitsDynamicStructureDNA1983","pages":"3231–3237","bibtex":"@article{Markovits1983,\n\ttitle = {Dynamic structure of {DNA} complexes. {Fluorometric} measurement of hydrogen-deuterium exchange kinetics of {DNA}-bound ethidium dimer and acridine-ethidium dimer},\n\tvolume = {22},\n\tissn = {0006-2960},\n\turl = {http://pubs.acs.org/doi/abs/10.1021/bi00282a030},\n\tdoi = {10.1021/bi00282a030},\n\tabstract = {The hydrogen-deuterium (H-D) exchange kinetics of free and DNA-bound ethidium dimer and acridine-ethidium heterodimer were measured by stopped flow using fluorescence detection. This technique allowed a very accurate measurement of the exchange process. The H-D exchange kinetics were measured in various environments. In some cases, it was observed that the H-D exchange was much faster than the dissociation rate of dimer-DNA complexes. This showed that the exchange was taking place directly from the bound state. Furthermore, the action of a catalyst (imidazolium ion) on the rate of H-D exchange showed that a dynamic structural fluctuation of the ligand in its DNA complex was a necessary step on the exchange process.},\n\tnumber = {13},\n\tjournal = {Biochemistry},\n\tauthor = {Markovits, J. and Ramstein, J. and Roques, B. P. and Pecq, J. B. Le},\n\tmonth = jun,\n\tyear = {1983},\n\tpmid = {6882747},\n\tnote = {tex.ids= markovitsDynamicStructureDNA1983},\n\tpages = {3231--3237},\n}\n\n","author_short":["Markovits, J.","Ramstein, J.","Roques, B. P.","Pecq, J. B. L."],"key":"Markovits1983","id":"Markovits1983","bibbaseid":"markovits-ramstein-roques-pecq-dynamicstructureofdnacomplexesfluorometricmeasurementofhydrogendeuteriumexchangekineticsofdnaboundethidiumdimerandacridineethidiumdimer-1983","role":"author","urls":{"Paper":"http://pubs.acs.org/doi/abs/10.1021/bi00282a030"},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":[],"search_terms":["dynamic","structure","dna","complexes","fluorometric","measurement","hydrogen","deuterium","exchange","kinetics","dna","bound","ethidium","dimer","acridine","ethidium","dimer","markovits","ramstein","roques","pecq"],"title":"Dynamic structure of DNA complexes. Fluorometric measurement of hydrogen-deuterium exchange kinetics of DNA-bound ethidium dimer and acridine-ethidium dimer","year":1983}