Identification of targeting peptides for the diagnosis of myocarditis. Martinez, M., Trac, D., Brown, M., Maher, K., & Davis, M. Nanomedicine (Lond), 13(7):787–801. Paper doi abstract bibtex AIM: Current diagnostic tests for myocarditis are invasive and have low diagnostic value. Our aim was to identify potential targeting peptides to detect early myocarditis following intravenous delivery. MATERIALS & METHODS: We used an animal model of experimental autoimmune myocarditis and a phage display library to identify potential targeting peptides. After several steps, we selected two peptides, MyH-PhD-05 and MyH-PhD-120, for in vivo screening using fluorescent imaging. Immunofluorescence and proteonomic analysis was used to identify potential cellular and molecular targets of MyH-PhD-05. Echocardiography was used to assess functional changes. RESULTS: Peptide MyH-PhD-05 was able to detect animals with severe myocarditis even in the absence of functional changes. Immunofluorescence demonstrated that MyH-PhD-05 colocalizes with CD4+ T cells and monocytes (CD11b+) in cardiac infiltrates. CONCLUSION: We identified potential targeting peptides for the diagnosis of myocarditis. Future studies will focus on better identification of potential targets and translating this technology to clinically relevant imaging modalities.
@article{martinez_identification_nodate,
title = {Identification of targeting peptides for the diagnosis of myocarditis.},
volume = {13},
url = {https://www.ncbi.nlm.nih.gov/pubmed/29473787},
doi = {10.2217/nnm-2018-0023},
abstract = {AIM: Current diagnostic tests for myocarditis are invasive and have low diagnostic value. Our aim was to identify potential targeting peptides to detect early myocarditis following intravenous delivery. MATERIALS \& METHODS: We used an animal model of experimental autoimmune myocarditis and a phage display library to identify potential targeting peptides. After several steps, we selected two peptides, MyH-PhD-05 and MyH-PhD-120, for in vivo screening using fluorescent imaging. Immunofluorescence and proteonomic analysis was used to identify potential cellular and molecular targets of MyH-PhD-05. Echocardiography was used to assess functional changes. RESULTS: Peptide MyH-PhD-05 was able to detect animals with severe myocarditis even in the absence of functional changes. Immunofluorescence demonstrated that MyH-PhD-05 colocalizes with CD4+ T cells and monocytes (CD11b+) in cardiac infiltrates. CONCLUSION: We identified potential targeting peptides for the diagnosis of myocarditis. Future studies will focus on better identification of potential targets and translating this technology to clinically relevant imaging modalities.},
language = {eng},
number = {7},
journal = {Nanomedicine (Lond)},
author = {Martinez, MD and Trac, DQ and Brown, ME and Maher, KO and Davis, ME},
keywords = {Peptides},
pages = {787--801}
}
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