Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities. Marzano, G.; Chiriaco, M. S.; Primiceri, E.; Dell'Aquila, M. E.; Ramalho-Santos, J.; Zara, V.; Ferramosca, A.; and Maruccio, G. BIOTECHNOLOGY ADVANCES, MAY-JUN, 2020.
doi  abstract   bibtex   
Male infertility often involves idiopathic or unknown causes, leading to an increasing demand for assisted reproduction technologies (ART). Conventional sperm sorting techniques rely on centrifugation steps that are known to cause oxidative stress and consequently damage cells. Alternative novel techniques have been introduced but offer disadvantages that need to be overcome. These techniques are also employed to increase the number and the quality of subjects in the animal breeding industry, to obtain purebred subjects or to preserve endangered animal species. Microfluidics deals with the manipulation of small amounts of volume within a microdevice known as lab-on-a-chip (LOC), which offers rapid analyses, ease of use, small reagent sample volumes, high-throughput processing and wide reproducibility owing to automation and standardization. As the LOC allows gamete handling within a microenvironment that strictly mimics physiological in vivo conditions and avoids centrifugation steps and long processing time, the use of microfluidics for sperm sorting and selection have been proposed during the last 15 years and is currently under investigation. Moreover, LOC technologies to sort, identify and analyse other kinds of cells could be transferred to sperm selection and analysis, thus opening the way to a novel approach to the sperm cell selection and manipulation. This review describes the techniques routinely performed in human and animal clinical practice for sorting good-quality sperm for in vitro fertilization procedures, and focuses on the positive and negative aspects of each method. Emerging microfluidic devices, recently proposed for sperm selection, are also described and, when possible, compared with standard methods.
@article{ ISI:000522635400016,
Author = {Marzano, Giuseppina and Chiriaco, Maria Serena and Primiceri, Elisabetta
   and Dell'Aquila, Maria Elena and Ramalho-Santos, Joao and Zara, Vincenzo
   and Ferramosca, Alessandra and Maruccio, Giuseppe},
Title = {{Sperm selection in assisted reproduction: A review of established
   methods and cutting-edge possibilities}},
Journal = {{BIOTECHNOLOGY ADVANCES}},
Year = {{2020}},
Volume = {{40}},
Month = {{MAY-JUN}},
Abstract = {{Male infertility often involves idiopathic or unknown causes, leading to
   an increasing demand for assisted reproduction technologies (ART).
   Conventional sperm sorting techniques rely on centrifugation steps that
   are known to cause oxidative stress and consequently damage cells.
   Alternative novel techniques have been introduced but offer
   disadvantages that need to be overcome. These techniques are also
   employed to increase the number and the quality of subjects in the
   animal breeding industry, to obtain purebred subjects or to preserve
   endangered animal species. Microfluidics deals with the manipulation of
   small amounts of volume within a microdevice known as lab-on-a-chip
   (LOC), which offers rapid analyses, ease of use, small reagent sample
   volumes, high-throughput processing and wide reproducibility owing to
   automation and standardization. As the LOC allows gamete handling within
   a microenvironment that strictly mimics physiological in vivo conditions
   and avoids centrifugation steps and long processing time, the use of
   microfluidics for sperm sorting and selection have been proposed during
   the last 15 years and is currently under investigation. Moreover, LOC
   technologies to sort, identify and analyse other kinds of cells could be
   transferred to sperm selection and analysis, thus opening the way to a
   novel approach to the sperm cell selection and manipulation. This review
   describes the techniques routinely performed in human and animal
   clinical practice for sorting good-quality sperm for in vitro
   fertilization procedures, and focuses on the positive and negative
   aspects of each method. Emerging microfluidic devices, recently proposed
   for sperm selection, are also described and, when possible, compared
   with standard methods.}},
DOI = {{10.1016/j.biotechadv.2019.107498}},
Article-Number = {{107498}},
ISSN = {{0734-9750}},
EISSN = {{1873-1899}},
ResearcherID-Numbers = {{Ramalho-Santos, Joao/K-8296-2019
   Ferramosca, Alessandra/B-8801-2015
   primiceri, elisabetta/P-8842-2015
   Chiriaco, Maria Serena/K-6082-2016
   Maruccio, Giuseppe/A-1486-2009}},
ORCID-Numbers = {{Ramalho-Santos, Joao/0000-0002-1172-4018
   Ferramosca, Alessandra/0000-0002-8251-9652
   primiceri, elisabetta/0000-0002-4196-3538
   Chiriaco, Maria Serena/0000-0002-7195-267X
   Maruccio, Giuseppe/0000-0001-7669-0253}},
Unique-ID = {{ISI:000522635400016}},
}
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