@article{
 title = {Papaya transformed with the Galanthus nivalis GNA gene produces a biologically active lectin with spider mite control activity},
 type = {article},
 keywords = {Carmine spider mite (Tetranychus cinnabarinus),Papaya (Carica papaya L.) transformation,Plant pest resistance,Snowdrop (Galanthus nivalis) lectin},
 volume = {In Press, },
 websites = {http://www.sciencedirect.com/science/article/B6TBH-4SJP77Y-1/1/d726d89ff7121f6087d5b8034824d9e0},
 id = {bfd6911f-43a2-3261-b7af-66cc0426ddbc},
 created = {2012-01-05T13:07:25.000Z},
 file_attached = {false},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2012-01-05T13:14:37.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
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 source_type = {Journal Article},
 abstract = {Snowdrop (Galanthus nivalis) lectin has previously been shown to have anti-feedant and insecticidal activity towards sap-sucking insects. However, its effectiveness against plant-parasitic mites has not been demonstrated. In this study, the commercial papaya (Carica papaya L.) cultivar Kapoho, which is highly susceptible to mites, was transformed with the snowdrop lectin (G. nivalis agglutin [GNA]) gene. Polymerase chain reaction confirmed the presence of the transgene and six independent transformed lines were selected for expression analysis. Western blot analysis showed that the lines expressed a recombinant protein with a molecular weight similar to that of the native snowdrop lectin. Leaf extracts containing the recombinant GNA protein agglutinated trypsinized rabbit erythrocytes thus, showing the GNA protein to be biologically active. ELISA and indirect measurement from the agglutination assay showed there to be variation in GNA expression among the lines produced. A laboratory bioassay using carmine spider mites (Tetranychus cinnabarinus) suggested improved pest resistance in the transgenic papaya plants. This is the first report that a transgenic plant expressing the GNA gene possesses enhanced resistance to a plant-parasitic mite.},
 bibtype = {article},
 author = {McCafferty, Heather R K and Moore, Paul H and Zhu, Yun J},
 journal = {Plant Science}
}

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