Tentacle development and regeneration in the Anthomedusa Polyorchis penicillatus and the involvement of the RF-amide immunoreactive neurons. McFadden, N. A. Master's thesis, University of Alberta (Canada), Canada, 1990.
Tentacle development and regeneration in the Anthomedusa Polyorchis penicillatus and the involvement of the RF-amide immunoreactive neurons [link]Paper  abstract   bibtex   
Tentacle development, and the coincident development of the RFamide-like (arg-phe amide-like) immunoreactive neurons in the hydromedusa Polyorchis penicillatus was described. In Stage 0, no bud was visible on the basis of external morphology, however it was known that a bud would form in this intertentacular region. There was no RFamide immunoreactivity in the region where the bud would form, other than the diffuse plexus of the intertentacular region. In Stage 1, the ectoderm thickened in the region of the future tentacle, forming a small bud at the bell margin. Within one day, in Stage 2, endoderm from the ring canal outpocketed into the ectodermal bud and formed the tentacle canal. Typically, there were 12-16 immunofluorescent cell bodies at this stage, which were arranged at the tip of the bud in one or two cup-like arrangements around the endodermal canal and extended neurites to the outer nerve-ring. After approximately 5-6 days the ocellus, or eyespot formed on the aboral surface of the tentacle bud. The formation of the ocellus characterized Stage 3. (Abstract shortened by UMI.)
@mastersthesis{mcfadden_tentacle_1990,
	address = {Canada},
	title = {Tentacle development and regeneration in the {Anthomedusa} {Polyorchis} penicillatus and the involvement of the {RF}-amide immunoreactive neurons},
	copyright = {Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.},
	url = {http://search.proquest.com.ezproxy.library.ubc.ca/pqdtglobal/docview/89142971/abstract/A76E964CF28B4249PQ/1},
	abstract = {Tentacle development, and the coincident development of the RFamide-like (arg-phe amide-like) immunoreactive neurons in the hydromedusa Polyorchis penicillatus was described. In Stage 0, no bud was visible on the basis of external morphology, however it was known that a bud would form in this intertentacular region. There was no RFamide immunoreactivity in the region where the bud would form, other than the diffuse plexus of the intertentacular region. In Stage 1, the ectoderm thickened in the region of the future tentacle, forming a small bud at the bell margin. Within one day, in Stage 2, endoderm from the ring canal outpocketed into the ectodermal bud and formed the tentacle canal. Typically, there were 12-16 immunofluorescent cell bodies at this stage, which were arranged at the tip of the bud in one or two cup-like arrangements around the endodermal canal and extended neurites to the outer nerve-ring. After approximately 5-6 days the ocellus, or eyespot formed on the aboral surface of the tentacle bud. The formation of the ocellus characterized Stage 3. (Abstract shortened by UMI.)},
	language = {English},
	urldate = {2016-06-09},
	school = {University of Alberta (Canada)},
	author = {McFadden, Nancy Aline},
	year = {1990},
	keywords = {Polyorchis penicillatus},
}
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