Paths to Attenuate Radiolysis-Induced Secondary Damage in Biological CryoEM. Mecklenburg, M., Nia, S. S, Saha, A., & Hong Zhou, Z Microscopy and Microanalysis, 30(Supplement_1):ozae044.877, July, 2024. doi abstract bibtex The imaging electrons in a transmission electron microscope are a beam of bond-breaking beta-radiation. Imaging soft materials is a challenge because of beam damage and poor contrast between the substrate and specimen. Typically, only 10-100 electrons can strike an atomically sized area before disintegration [1]. Peptide, hydrogen, disulfide bonds etc. are all irreparably broken by radiolysis while being simultaneously imaged. The total dose for loss of useful information is roughly the Henderson limit (often accumulated in a few seconds, see Figure 1), about 20 MGy [2], which is roughly 9 orders of magnitude larger than an average person receives per year [3]. This primary damage cannot be abated in small molecules, protein, or virus samples. In addition, unlike the case for x-rays, the inelastic scattering is more likely than elastic scattering for elements in atomic number less than about iron [4]. This leads to poor contrast using parallel beam imaging modalities.
@article{mecklenburgPathsAttenuateRadiolysisInduced2024,
title = {Paths to {{Attenuate Radiolysis-Induced Secondary Damage}} in {{Biological CryoEM}}},
author = {Mecklenburg, Matthew and Nia, Shervin S and Saha, Ambarneil and Hong Zhou, Z},
year = {2024},
month = jul,
journal = {Microscopy and Microanalysis},
volume = {30},
number = {Supplement\_1},
pages = {ozae044.877},
issn = {1431-9276},
doi = {10.1093/mam/ozae044.877},
urldate = {2025-04-04},
abstract = {The imaging electrons in a transmission electron microscope are a beam of bond-breaking beta-radiation. Imaging soft materials is a challenge because of beam damage and poor contrast between the substrate and specimen. Typically, only 10-100 electrons can strike an atomically sized area before disintegration [1]. Peptide, hydrogen, disulfide bonds etc. are all irreparably broken by radiolysis while being simultaneously imaged. The total dose for loss of useful information is roughly the Henderson limit (often accumulated in a few seconds, see Figure 1), about 20 MGy [2], which is roughly 9 orders of magnitude larger than an average person receives per year [3]. This primary damage cannot be abated in small molecules, protein, or virus samples. In addition, unlike the case for x-rays, the inelastic scattering is more likely than elastic scattering for elements in atomic number less than about iron [4]. This leads to poor contrast using parallel beam imaging modalities.},
file = {C\:\\Users\\shervinnia\\Zotero\\storage\\ZXD7QZSK\\Mecklenburg et al. - 2024 - Paths to Attenuate Radiolysis-Induced Secondary Damage in Biological CryoEM.pdf;C\:\\Users\\shervinnia\\Zotero\\storage\\C67US3J9\\7720210.html}
}
Downloads: 0
{"_id":"voP4C3KSGLR9oBrz5","bibbaseid":"mecklenburg-nia-saha-hongzhou-pathstoattenuateradiolysisinducedsecondarydamageinbiologicalcryoem-2024","author_short":["Mecklenburg, M.","Nia, S. S","Saha, A.","Hong Zhou, Z"],"bibdata":{"bibtype":"article","type":"article","title":"Paths to Attenuate Radiolysis-Induced Secondary Damage in Biological CryoEM","author":[{"propositions":[],"lastnames":["Mecklenburg"],"firstnames":["Matthew"],"suffixes":[]},{"propositions":[],"lastnames":["Nia"],"firstnames":["Shervin","S"],"suffixes":[]},{"propositions":[],"lastnames":["Saha"],"firstnames":["Ambarneil"],"suffixes":[]},{"propositions":[],"lastnames":["Hong","Zhou"],"firstnames":["Z"],"suffixes":[]}],"year":"2024","month":"July","journal":"Microscopy and Microanalysis","volume":"30","number":"Supplement_1","pages":"ozae044.877","issn":"1431-9276","doi":"10.1093/mam/ozae044.877","urldate":"2025-04-04","abstract":"The imaging electrons in a transmission electron microscope are a beam of bond-breaking beta-radiation. Imaging soft materials is a challenge because of beam damage and poor contrast between the substrate and specimen. Typically, only 10-100 electrons can strike an atomically sized area before disintegration [1]. Peptide, hydrogen, disulfide bonds etc. are all irreparably broken by radiolysis while being simultaneously imaged. The total dose for loss of useful information is roughly the Henderson limit (often accumulated in a few seconds, see Figure 1), about 20 MGy [2], which is roughly 9 orders of magnitude larger than an average person receives per year [3]. This primary damage cannot be abated in small molecules, protein, or virus samples. In addition, unlike the case for x-rays, the inelastic scattering is more likely than elastic scattering for elements in atomic number less than about iron [4]. This leads to poor contrast using parallel beam imaging modalities.","file":"C\\:\\\\Users\\\\shervinnia\\\\Zotero\\\\storage\\\\ZXD7QZSK\\\\Mecklenburg et al. - 2024 - Paths to Attenuate Radiolysis-Induced Secondary Damage in Biological CryoEM.pdf;C\\:\\\\Users\\\\shervinnia\\\\Zotero\\\\storage\\\\C67US3J9\\\\7720210.html","bibtex":"@article{mecklenburgPathsAttenuateRadiolysisInduced2024,\n title = {Paths to {{Attenuate Radiolysis-Induced Secondary Damage}} in {{Biological CryoEM}}},\n author = {Mecklenburg, Matthew and Nia, Shervin S and Saha, Ambarneil and Hong Zhou, Z},\n year = {2024},\n month = jul,\n journal = {Microscopy and Microanalysis},\n volume = {30},\n number = {Supplement\\_1},\n pages = {ozae044.877},\n issn = {1431-9276},\n doi = {10.1093/mam/ozae044.877},\n urldate = {2025-04-04},\n abstract = {The imaging electrons in a transmission electron microscope are a beam of bond-breaking beta-radiation. Imaging soft materials is a challenge because of beam damage and poor contrast between the substrate and specimen. Typically, only 10-100 electrons can strike an atomically sized area before disintegration [1]. Peptide, hydrogen, disulfide bonds etc. are all irreparably broken by radiolysis while being simultaneously imaged. The total dose for loss of useful information is roughly the Henderson limit (often accumulated in a few seconds, see Figure 1), about 20 MGy [2], which is roughly 9 orders of magnitude larger than an average person receives per year [3]. This primary damage cannot be abated in small molecules, protein, or virus samples. In addition, unlike the case for x-rays, the inelastic scattering is more likely than elastic scattering for elements in atomic number less than about iron [4]. This leads to poor contrast using parallel beam imaging modalities.},\n file = {C\\:\\\\Users\\\\shervinnia\\\\Zotero\\\\storage\\\\ZXD7QZSK\\\\Mecklenburg et al. - 2024 - Paths to Attenuate Radiolysis-Induced Secondary Damage in Biological CryoEM.pdf;C\\:\\\\Users\\\\shervinnia\\\\Zotero\\\\storage\\\\C67US3J9\\\\7720210.html}\n}\n\n","author_short":["Mecklenburg, M.","Nia, S. S","Saha, A.","Hong Zhou, Z"],"key":"mecklenburgPathsAttenuateRadiolysisInduced2024","id":"mecklenburgPathsAttenuateRadiolysisInduced2024","bibbaseid":"mecklenburg-nia-saha-hongzhou-pathstoattenuateradiolysisinducedsecondarydamageinbiologicalcryoem-2024","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/2MtabhipmJFGQ7n9n","dataSources":["mrv2RSxFMmgGBMu4y"],"keywords":[],"search_terms":["paths","attenuate","radiolysis","induced","secondary","damage","biological","cryoem","mecklenburg","nia","saha","hong zhou"],"title":"Paths to Attenuate Radiolysis-Induced Secondary Damage in Biological CryoEM","year":2024}