@article{mehmood_physical_2019,
	title = {Physical and chemical mutagens improved \textit{{Sporotrichum} thermophile}, strain {ST20} for enhanced {Phytase} activity},
	volume = {26},
	issn = {1319-562X},
	url = {https://www.sciencedirect.com/science/article/pii/S1319562X1930124X},
	doi = {10.1016/j.sjbs.2019.07.006},
	abstract = {Objective
Phosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of phosphate solubilizing microorganisms such as bacteria and fungi. The phytases are widespread in an environment and have been isolated from different sources comprising bacteria and fungi.
Methodology
In current studies, we show the successful use of gamma rays and EMS (Ethyl Methane Sulphonate) mutagenesis for enhanced activity of phytases in a fungal strain Sporotrichum thermophile.
Results
We report an improved strain ST2 that could produce a clear halo zone around the colony, up to 24 mm. The maximum enzymatic activity was found of 382 U/mL on pH 5.5. However, the phytase activity was improved to 387 U/ml at 45 °C. We also report that the mutants produced through EMS showed the greater potential for phytase production.
Conclusion
The current study highlights the potential of EMS mutagenesis for strain improvement over physical mutagens.},
	number = {7},
	urldate = {2024-08-30},
	journal = {Saudi Journal of Biological Sciences},
	author = {Mehmood, Asim and Baneen, Umal and Zaheer, Ahmad and Wasim Sajid, Muhammad and Hussain, Abrar and Saleem, Shahzad and Yousafi, Qudsia and Rashid, Hamid and Riaz, Hassan and Ihsan, Awais and Jamil, Farrukh and Sajjad, Yasar and Zahid, Nageena and Anjam, Muhammad Shahzad and Arshad, Muhammad and Mirza, Zeenat and Karim, Sajjad and Rasool, Mahmood},
	month = nov,
	year = {2019},
	keywords = {EMS mutagenesis, Fungus, Gamma rays, Phosphate solubilizer},
	pages = {1485--1491},
}

{"_id":"Jam4cZn8z863zWdwB","bibbaseid":"mehmood-baneen-zaheer-wasimsajid-hussain-saleem-yousafi-rashid-etal-physicalandchemicalmutagensimprovedisporotrichumthermophileistrainst20forenhancedphytaseactivity-2019","author_short":["Mehmood, A.","Baneen, U.","Zaheer, A.","Wasim Sajid, M.","Hussain, A.","Saleem, S.","Yousafi, Q.","Rashid, H.","Riaz, H.","Ihsan, A.","Jamil, F.","Sajjad, Y.","Zahid, N.","Anjam, M. S.","Arshad, M.","Mirza, Z.","Karim, S.","Rasool, M."],"bibdata":{"bibtype":"article","type":"article","title":"Physical and chemical mutagens improved <i>Sporotrichum thermophile</i>, strain ST20 for enhanced Phytase activity","volume":"26","issn":"1319-562X","url":"https://www.sciencedirect.com/science/article/pii/S1319562X1930124X","doi":"10.1016/j.sjbs.2019.07.006","abstract":"Objective Phosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of phosphate solubilizing microorganisms such as bacteria and fungi. The phytases are widespread in an environment and have been isolated from different sources comprising bacteria and fungi. Methodology In current studies, we show the successful use of gamma rays and EMS (Ethyl Methane Sulphonate) mutagenesis for enhanced activity of phytases in a fungal strain Sporotrichum thermophile. Results We report an improved strain ST2 that could produce a clear halo zone around the colony, up to 24 mm. The maximum enzymatic activity was found of 382 U/mL on pH 5.5. However, the phytase activity was improved to 387 U/ml at 45 °C. We also report that the mutants produced through EMS showed the greater potential for phytase production. Conclusion The current study highlights the potential of EMS mutagenesis for strain improvement over physical mutagens.","number":"7","urldate":"2024-08-30","journal":"Saudi Journal of Biological Sciences","author":[{"propositions":[],"lastnames":["Mehmood"],"firstnames":["Asim"],"suffixes":[]},{"propositions":[],"lastnames":["Baneen"],"firstnames":["Umal"],"suffixes":[]},{"propositions":[],"lastnames":["Zaheer"],"firstnames":["Ahmad"],"suffixes":[]},{"propositions":[],"lastnames":["Wasim","Sajid"],"firstnames":["Muhammad"],"suffixes":[]},{"propositions":[],"lastnames":["Hussain"],"firstnames":["Abrar"],"suffixes":[]},{"propositions":[],"lastnames":["Saleem"],"firstnames":["Shahzad"],"suffixes":[]},{"propositions":[],"lastnames":["Yousafi"],"firstnames":["Qudsia"],"suffixes":[]},{"propositions":[],"lastnames":["Rashid"],"firstnames":["Hamid"],"suffixes":[]},{"propositions":[],"lastnames":["Riaz"],"firstnames":["Hassan"],"suffixes":[]},{"propositions":[],"lastnames":["Ihsan"],"firstnames":["Awais"],"suffixes":[]},{"propositions":[],"lastnames":["Jamil"],"firstnames":["Farrukh"],"suffixes":[]},{"propositions":[],"lastnames":["Sajjad"],"firstnames":["Yasar"],"suffixes":[]},{"propositions":[],"lastnames":["Zahid"],"firstnames":["Nageena"],"suffixes":[]},{"propositions":[],"lastnames":["Anjam"],"firstnames":["Muhammad","Shahzad"],"suffixes":[]},{"propositions":[],"lastnames":["Arshad"],"firstnames":["Muhammad"],"suffixes":[]},{"propositions":[],"lastnames":["Mirza"],"firstnames":["Zeenat"],"suffixes":[]},{"propositions":[],"lastnames":["Karim"],"firstnames":["Sajjad"],"suffixes":[]},{"propositions":[],"lastnames":["Rasool"],"firstnames":["Mahmood"],"suffixes":[]}],"month":"November","year":"2019","keywords":"EMS mutagenesis, Fungus, Gamma rays, Phosphate solubilizer","pages":"1485–1491","bibtex":"@article{mehmood_physical_2019,\n\ttitle = {Physical and chemical mutagens improved \\textit{{Sporotrichum} thermophile}, strain {ST20} for enhanced {Phytase} activity},\n\tvolume = {26},\n\tissn = {1319-562X},\n\turl = {https://www.sciencedirect.com/science/article/pii/S1319562X1930124X},\n\tdoi = {10.1016/j.sjbs.2019.07.006},\n\tabstract = {Objective\nPhosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of phosphate solubilizing microorganisms such as bacteria and fungi. The phytases are widespread in an environment and have been isolated from different sources comprising bacteria and fungi.\nMethodology\nIn current studies, we show the successful use of gamma rays and EMS (Ethyl Methane Sulphonate) mutagenesis for enhanced activity of phytases in a fungal strain Sporotrichum thermophile.\nResults\nWe report an improved strain ST2 that could produce a clear halo zone around the colony, up to 24 mm. The maximum enzymatic activity was found of 382 U/mL on pH 5.5. However, the phytase activity was improved to 387 U/ml at 45 °C. We also report that the mutants produced through EMS showed the greater potential for phytase production.\nConclusion\nThe current study highlights the potential of EMS mutagenesis for strain improvement over physical mutagens.},\n\tnumber = {7},\n\turldate = {2024-08-30},\n\tjournal = {Saudi Journal of Biological Sciences},\n\tauthor = {Mehmood, Asim and Baneen, Umal and Zaheer, Ahmad and Wasim Sajid, Muhammad and Hussain, Abrar and Saleem, Shahzad and Yousafi, Qudsia and Rashid, Hamid and Riaz, Hassan and Ihsan, Awais and Jamil, Farrukh and Sajjad, Yasar and Zahid, Nageena and Anjam, Muhammad Shahzad and Arshad, Muhammad and Mirza, Zeenat and Karim, Sajjad and Rasool, Mahmood},\n\tmonth = nov,\n\tyear = {2019},\n\tkeywords = {EMS mutagenesis, Fungus, Gamma rays, Phosphate solubilizer},\n\tpages = {1485--1491},\n}\n\n\n\n","author_short":["Mehmood, A.","Baneen, U.","Zaheer, A.","Wasim Sajid, M.","Hussain, A.","Saleem, S.","Yousafi, Q.","Rashid, H.","Riaz, H.","Ihsan, A.","Jamil, F.","Sajjad, Y.","Zahid, N.","Anjam, M. S.","Arshad, M.","Mirza, Z.","Karim, S.","Rasool, M."],"key":"mehmood_physical_2019","id":"mehmood_physical_2019","bibbaseid":"mehmood-baneen-zaheer-wasimsajid-hussain-saleem-yousafi-rashid-etal-physicalandchemicalmutagensimprovedisporotrichumthermophileistrainst20forenhancedphytaseactivity-2019","role":"author","urls":{"Paper":"https://www.sciencedirect.com/science/article/pii/S1319562X1930124X"},"keyword":["EMS mutagenesis","Fungus","Gamma rays","Phosphate solubilizer"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["3zTPPmKj8BiTcpc6C","9cGcv2t8pRzC92kzs"],"keywords":["ems mutagenesis","fungus","gamma rays","phosphate solubilizer"],"search_terms":["physical","chemical","mutagens","improved","sporotrichum","thermophile","strain","st20","enhanced","phytase","activity","mehmood","baneen","zaheer","wasim sajid","hussain","saleem","yousafi","rashid","riaz","ihsan","jamil","sajjad","zahid","anjam","arshad","mirza","karim","rasool"],"title":"Physical and chemical mutagens improved <i>Sporotrichum thermophile</i>, strain ST20 for enhanced Phytase activity","year":2019}