@article{RN136,
   author = {Mercadal, B. and Beitel-White, N. and Aycock, K. N. and Castellví, Q. and Davalos, R. V. and Ivorra, A.},
   title = {Dynamics of Cell Death After Conventional IRE and H-FIRE Treatments},
   journal = {Ann Biomed Eng},
   volume = {48},
   number = {5},
   pages = {1451-1462},
   note = {1573-9686
Mercadal, Borja
Beitel-White, Natalie
Aycock, Kenneth N
Castellví, Quim
Davalos, Rafael V
Ivorra, Antoni
Orcid: 0000-0001-7718-8767
TEC2014-52383-C3-2-R/Ministerio de Economía y Competitividad/
PUJFSANY/Cures Within Reach/
16-65-IANN/PCAN/Pancreatic Cancer Action Network/United States
Journal Article
United States
2020/02/07
Ann Biomed Eng. 2020 May;48(5):1451-1462. doi: 10.1007/s10439-020-02462-8. Epub 2020 Feb 5.},
   abstract = {High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.},
   keywords = {Caspase 3/metabolism
Caspase 7/metabolism
Cell Culture Techniques
*Cell Death
Cell Line, Tumor
Electroporation/*methods
Humans
Bipolar pulses
Caspase 3/7
High-frequency irreversible electroporation
Irreversible electroporation
Membrane permeability},
   ISSN = {0090-6964 (Print)
0090-6964},
   DOI = {10.1007/s10439-020-02462-8},
   year = {2020},
   type = {Journal Article}
}

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V.","Ivorra, A."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Mercadal"],"firstnames":["B."],"suffixes":[]},{"propositions":[],"lastnames":["Beitel-White"],"firstnames":["N."],"suffixes":[]},{"propositions":[],"lastnames":["Aycock"],"firstnames":["K.","N."],"suffixes":[]},{"propositions":[],"lastnames":["Castellví"],"firstnames":["Q."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Ivorra"],"firstnames":["A."],"suffixes":[]}],"title":"Dynamics of Cell Death After Conventional IRE and H-FIRE Treatments","journal":"Ann Biomed Eng","volume":"48","number":"5","pages":"1451-1462","note":"1573-9686 Mercadal, Borja Beitel-White, Natalie Aycock, Kenneth N Castellví, Quim Davalos, Rafael V Ivorra, Antoni Orcid: 0000-0001-7718-8767 TEC2014-52383-C3-2-R/Ministerio de Economía y Competitividad/ PUJFSANY/Cures Within Reach/ 16-65-IANN/PCAN/Pancreatic Cancer Action Network/United States Journal Article United States 2020/02/07 Ann Biomed Eng. 2020 May;48(5):1451-1462. doi: 10.1007/s10439-020-02462-8. Epub 2020 Feb 5.","abstract":"High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.","keywords":"Caspase 3/metabolism Caspase 7/metabolism Cell Culture Techniques *Cell Death Cell Line, Tumor Electroporation/*methods Humans Bipolar pulses Caspase 3/7 High-frequency irreversible electroporation Irreversible electroporation Membrane permeability","issn":"0090-6964 (Print) 0090-6964","doi":"10.1007/s10439-020-02462-8","year":"2020","bibtex":"@article{RN136,\n author = {Mercadal, B. and Beitel-White, N. and Aycock, K. N. and Castellví, Q. and Davalos, R. V. and Ivorra, A.},\n title = {Dynamics of Cell Death After Conventional IRE and H-FIRE Treatments},\n journal = {Ann Biomed Eng},\n volume = {48},\n number = {5},\n pages = {1451-1462},\n note = {1573-9686\nMercadal, Borja\nBeitel-White, Natalie\nAycock, Kenneth N\nCastellví, Quim\nDavalos, Rafael V\nIvorra, Antoni\nOrcid: 0000-0001-7718-8767\nTEC2014-52383-C3-2-R/Ministerio de Economía y Competitividad/\nPUJFSANY/Cures Within Reach/\n16-65-IANN/PCAN/Pancreatic Cancer Action Network/United States\nJournal Article\nUnited States\n2020/02/07\nAnn Biomed Eng. 2020 May;48(5):1451-1462. doi: 10.1007/s10439-020-02462-8. Epub 2020 Feb 5.},\n abstract = {High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. 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