Kinetics of double-chain reversals bridging contiguous quartets in tetramolecular quadruplexes. Mergny, J., Cian, A. D., Amrane, S., & da Silva, M. W. Nucleic acids research, 34(8):2386–97, January, 2006. ![Kinetics of double-chain reversals bridging contiguous quartets in tetramolecular quadruplexes. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Repetitive 5'GGXGG DNA segments abound in, or near, regulatory regions of the genome and may form unusual structures called G-quadruplexes. Using NMR spectroscopy, we demonstrate that a family of 5'GCGGXGGY sequences adopts a folding topology containing double-chain reversals. The topology is composed of two bistranded quadruplex monomeric units linked by formation of G:C:G:C tetrads. We provide a complete thermodynamic and kinetic analysis of 13 different sequences using absorbance spectroscopy and DSC, and compare their kinetics with a canonical tetrameric parallel-stranded quadruplex formed by TG4T. We demonstrate large differences (up to 10(5)-fold) in the association constants of these quadruplexes depending on primary sequence; the fastest samples exhibiting association rate equal or higher than the canonical TG4T quadruplex. In contrast, all sequences studied here unfold at a lower temperature than this quadruplex. Some sequences have thermodynamic stability comparable to the canonical TG4T tetramolecular quadruplex, but with faster association and dissociation. Sequence effects on the dissociation processes are discussed in light of structural data.
@article{Mergny2006,
title = {Kinetics of double-chain reversals bridging contiguous quartets in tetramolecular quadruplexes.},
volume = {34},
issn = {1362-4962},
url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1458523&tool=pmcentrez&rendertype=abstract},
doi = {10.1093/nar/gkl098},
abstract = {Repetitive 5'GGXGG DNA segments abound in, or near, regulatory regions of the genome and may form unusual structures called G-quadruplexes. Using NMR spectroscopy, we demonstrate that a family of 5'GCGGXGGY sequences adopts a folding topology containing double-chain reversals. The topology is composed of two bistranded quadruplex monomeric units linked by formation of G:C:G:C tetrads. We provide a complete thermodynamic and kinetic analysis of 13 different sequences using absorbance spectroscopy and DSC, and compare their kinetics with a canonical tetrameric parallel-stranded quadruplex formed by TG4T. We demonstrate large differences (up to 10(5)-fold) in the association constants of these quadruplexes depending on primary sequence; the fastest samples exhibiting association rate equal or higher than the canonical TG4T quadruplex. In contrast, all sequences studied here unfold at a lower temperature than this quadruplex. Some sequences have thermodynamic stability comparable to the canonical TG4T tetramolecular quadruplex, but with faster association and dissociation. Sequence effects on the dissociation processes are discussed in light of structural data.},
number = {8},
journal = {Nucleic acids research},
author = {Mergny, Jean-Louis and Cian, Anne De and Amrane, Samir and da Silva, Mateus Webba},
month = jan,
year = {2006},
pmid = {16682446},
keywords = {\#nosource, Base Sequence, Biomolecular, Calorimetry, DNA, DNA: chemistry, Differential Scanning, G-Quadruplexes, Guanine, Guanine: chemistry, Hydrogen-Ion Concentration, Kinetics, Nuclear Magnetic Resonance, Nucleic Acid Conformation, Osmolar Concentration, Temperature, Thermodynamics},
pages = {2386--97},
}
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