Following G-quartet formation by UV-spectroscopy. Mergny, J L, Phan, a T, & Lacroix, L FEBS letters, 435(1):74–8, October, 1998. ![Following G-quartet formation by UV-spectroscopy. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper abstract bibtex Oligodeoxynucleotides which include stretches of guanines form a well-known tetrameric structure. We show that the recording of reversible absorbance changes at 295 nm allows to precisely monitor intramolecular guanine (G)-quartet formation and dissociation. Accurate Tm and thermodynamic values could be easily extracted from the data, whereas classical recordings at 260 nm led to a much larger uncertainty and in extreme cases, to completely inaccurate measurements. This inverted denaturation profile was observed for all G-quartet-forming oligonucleotides studied so far. This technique is very useful in all cases where intramolecular or intermolecular quadruplex formation is suspected.
@article{Mergny1998,
title = {Following {G}-quartet formation by {UV}-spectroscopy.},
volume = {435},
issn = {0014-5793},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9755862},
abstract = {Oligodeoxynucleotides which include stretches of guanines form a well-known tetrameric structure. We show that the recording of reversible absorbance changes at 295 nm allows to precisely monitor intramolecular guanine (G)-quartet formation and dissociation. Accurate Tm and thermodynamic values could be easily extracted from the data, whereas classical recordings at 260 nm led to a much larger uncertainty and in extreme cases, to completely inaccurate measurements. This inverted denaturation profile was observed for all G-quartet-forming oligonucleotides studied so far. This technique is very useful in all cases where intramolecular or intermolecular quadruplex formation is suspected.},
number = {1},
journal = {FEBS letters},
author = {Mergny, J L and Phan, a T and Lacroix, L},
month = oct,
year = {1998},
pmid = {9755862},
keywords = {\#nosource, Antisense, Antisense: chemistry, DNA, DNA: chemistry, Dimerization, G-Quadruplexes, Guanine, Guanine: chemistry, HIV-1, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Oligodeoxyribonucleotides: chemistry, Oligonucleotides, RNA, Spectrophotometry, Thermodynamics, Ultraviolet, Viral, Viral: chemistry},
pages = {74--8},
}
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